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EC number: 306-479-5 | CAS number: 97280-83-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: According to current OECD guideline, but without GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- Both a standard plate test and a preincubation test (with and without metabolic activation) were carried out.
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Dodecene, branched
- EC Number:
- 306-479-5
- EC Name:
- Dodecene, branched
- Cas Number:
- 97280-83-6
- Molecular formula:
- C12 H24
- IUPAC Name:
- (2Z)-4-methylundec-2-ene
- Details on test material:
- - Name of test material (as cited in study report): Isododecene
- Analytical purity: 99%
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S 9 fraction (liver of Aroclor 1254 induced rats)
- Test concentrations with justification for top dose:
- 1st experiment - standard plate test: All strains - 0, 20, 100, 500, 2500 and 5000 µg/plate
2nd experiment - standard plate test: TA 1537 - 0, 100, 500, 2500, 5000 and 7500 µg/plate
3rd experiment - preincubation method: All strains - 0, 20, 100, 500, 2500 and 5000 µg/plate - Vehicle / solvent:
- Acetone
Controls
- Untreated negative controls:
- yes
- Remarks:
- parallel with each experiment for each tester strain with and without metabolic activation
- Negative solvent / vehicle controls:
- yes
- Remarks:
- parallel with each experiment for each tester strain with and without metabolic activation
- Positive controls:
- yes
- Remarks:
- with S 9 mix: 2-aminoanthracene for all strains; without S 9 mix: N-methyl-N´-nitro-N-nitrosoguanidine (dissolved in DMSO) for TA100 and TA 1535, 4-nitro-o-phenylendiamine for TA98 and 9-aminoacridine for TA1537
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation) and preincubation
Preincubation method:
DURATION
- Preincubation period: 20 min
- Exposure duration: 48 hours
Number of plates: 3 test plates per dose or per control each - Evaluation criteria:
- In general, a substance to be characterized as positive in the Ames test has to fulfill the following requirements:
- doubling of the spontaneous mutation rate (control)
- dose-response relationshi p
- reproducibility of the results .
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: see Additional information on results
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- ADDITIONAL INFORMATION ON CYTOTOXICITY:
A weakly bacteriotoxic effect ( reduced his background growth, slight decrease in the number of his+ revertants) was only observed in the preincubation test withou t S-9 mix using TA 100 at 5000 µg/plate and with TA 1537 from about 500 Ng/plate onward .
Any other information on results incl. tables
1st experiment (standard plate test)
TA 1535 | TA 1537 | TA 98 | TA 100 | ||||||
Quotient + S9 | Quotient - S9 | Quotient + S9 | Quotient - S9 | Quotient + S9 | Quotient - S9 | Quotient +S9 | Quotient-S9 | ||
Acetone | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | |
20 µg/plate | 1.1 | 0.7 | 1.7 | 0.6 | 1.1 | 0.6 | 1.0 | 0.9 | |
100 µg/plate | 1.3 | 1.0 | 1.9 | 0.6 | 1.0 | 0.8 | 1.0 | 1.0 | |
500 µg/plate | 1.0 | 1.0 | 1.9 | 0.8 | 1.0 | 1.1 | 0.9 | 1.0 | |
2500 µg/plate | 1.2 | 0.9 | 1.8 | 0.7 | 1.0 | 1.3 | 1.0 | 0.9 | |
5000 µg/plate | 1.1 | 0.9 | 2.0 | 0.8 | 1.0 | 1.1 | 1.0 | 0.9 | |
positive conrol | 66.1 | 11.8 | 12.5 | 37.6 | 28.1 | 45.4 | 13.3 | 12.4 |
3rd experiment (standard plate test)
TA 1537 | ||
Quotient +S9 | Quotient -S9 | |
Acetone | 1.0 | 1.0 |
100 µg/plate | 1.0 | 1.0 |
500 µg/plate | 1.0 | 1.0 |
2500 µg/plate | 0.9 | 0.7 |
5000 µg/plate | 1.1 | 0.7 |
7500 µg/plate | 1.0 | 0.9 |
positive control | 14.4 | 66 .3 |
2nd experiment (preincubation method)
TA 1535 | TA 1537 | TA 98 | TA 100 | ||||||
Quotient + S9 | Quotient - S9 | Quotient + S9 | Quotient - S9 | Quotient + S9 | Quotient - S9 | Quotient +S9 | Quotient-S9 | ||
Acetone | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | |
20 µg/plate | 1.0 | 1.3 | 0.9 | 1.4 | 1.0 | 0.8 | 1.1 | 1.1 | |
100 µg/plate | 0.7 | 1.4 | 0.8 | 0.8 | 1.0 | 0.7 | 1.0 | 1.0 | |
500 µg/plate | 0.8 | 1.3 | 0.8 | 1.1 | 0.9 | 0.7 | 0.9 | 0.9 | |
2500 µg/plate | 0.7 | 1.5 | 0.6 | 0.5 | 0.7 | 0.8 | 1.0 | 0.8 | |
5000 µg/plate | 0.8 | 1.3 | 0.7 | 0.7 | 0.6 | 0.7 | 0.9 | 0.6 | |
positive conrol | 8.6 | 75.4 | 10.4 | 65.8 | 15.6 | 35 .8 | 7.2 | 7.1 |
According to the results of the present study, the test substance is not mutagenic in the Ames test .
The slight and not dose-dependent increase in the number of revertant colonies using the strain TA 1537 in the 1st standard plate test with S-9 mix could not be confirmed either in a 2nd standard plate test including higher doses or in a preincubation assay . Thus, the findings of the tst study with TA 1537 are regarded as incidental .
Applicant's summary and conclusion
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