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EC number: 221-220-5 | CAS number: 3033-62-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1985-06-12 - 1985-06-28
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: Internal laboratory standard protocol
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- no
- Principles of method if other than guideline:
- No guideline methods were documented in the study report, although the study was conducted according to the following protocol. The test substance was maintained in contact with shaven trunk skin of male and female New Zealand White rabbits, for a either 4 or 24 hours, using an occlusive dressing. Groups containing 5 animals were used, and rabbits were observed for 14 days after application of test substance to the skin. Studies were conducted using undiluted test substance with contact periods of 4 and 24 hr, and a 20% aqueous solution (v/v) maintained in contact for 24 hr. LD50's were calculated by the moving average method and are based on a 14-day observation period. Animal weights were recorded at 0 days (before dose), 7 days and 14 days (just prior to sacrifice). At death or sacrifice, each animal is subjected to gross pathologic evaluation.
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- N,N,N',N'-tetramethyl-2,2'-oxybis(ethylamine)
- EC Number:
- 221-220-5
- EC Name:
- N,N,N',N'-tetramethyl-2,2'-oxybis(ethylamine)
- Cas Number:
- 3033-62-3
- Molecular formula:
- C8H20N2O
- IUPAC Name:
- {2-[2-(dimethylamino)ethoxy]ethyl}dimethylamine
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): N,N,N',N'-tetramethyl-2,2'-oxybis(ethylamine)
- Molecular formula (if other than submission substance): (CH3)2NCH2CH2OCH2CH2N(CH3)2
- Molecular weight (if other than submission substance): 160.26
- Substance type: Pure active substance
- Physical state: Yellow, transparent, slightly viscous liquid
- Analytical purity: N/A
- Impurities (identity and concentrations): N/A
- Composition of test material, percentage of components: N/A
- Purity test date: N/A
- Expiration date of the lot/batch: N/A
- Storage condition of test material: N/A
- Other: N/A
- Lot/batch No.: Identification: S-867907; Charge No.: 502670-0000-2286; BRRC Sample No. 48-97
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: no data
- Age at study initiation: no data
- Weight at study initiation: 2.0 and 3.0 kg
- Fasting period before study: Yes, during exposure
- Housing: no data
- Diet (e.g. ad libitum): Commercial diet, ad libitum
- Water (e.g. ad libitum): Municipal water, ad libitum
- Acclimation period: 24 hours
ENVIRONMENTAL CONDITIONS
- Temperature (°C): no data
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): no data
IN-LIFE DATES: From: 1985-06-12 To: 1985-06-26
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- other: 4 and 24 hour test were unchanged test substance; 24 hour test with 20% dilution with distilled water.
- Details on dermal exposure:
- TEST SITE
- Area of exposure: Trunk (clipped and intact)
- % coverage: no data
- Type of wrap if used: Impervious sheeting
REMOVAL OF TEST SUBSTANCE
- Washing (if done): Yes, after the contact period, excess fluid was removed to diminish ingestion.
- Time after start of exposure: no data
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): no data
- Concentration (if solution): pure substance and 20% solution
- Constant volume or concentration used: No, doses were varied by adjusting the volume or weight of the test substance.
- For solids, paste formed: not applicable
VEHICLE
- Amount(s) applied (volume or weight with unit): no data
- Concentration (if solution): no data
- Lot/batch no. (if required): no data
- Purity: no data - Duration of exposure:
- 4 or 24 hour exposure periods.
- Doses:
- - 4-hour contact period: 1.00, 0.50, 0.25 mL/kg (Males) and 1.00, 0.71, 0.50 mL/kg (Females).
- 24-hours contact period: 0.80, 0.40, 0.20 mL/kg (Males) and 0.80, 0.40, 0.20, 0.10 mL/kg (Females)
- 24-hours contact period with 20% dilution: 4.00, 2.00, 1.00 mL/kg (Males) and 8.00, 4.00, 2.00 mL/kg (Females) - No. of animals per sex per dose:
- 5/sex/dose
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Animal weights were recorded at 0 days (before dose), 7 days and 14 days (just prior to sacrifice).
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, and gross pathology - Statistics:
- LD50's are calculated by the moving average method and are based on a 14-day observation period.
Results and discussion
- Preliminary study:
- N/A
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 0.406 mL/kg bw
- Based on:
- test mat.
- 95% CL:
- 0.295 - 0.559
- Remarks on result:
- other: 4-hr Contact with Undiluted Sample; Rabbit
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 0.633 mL/kg bw
- Based on:
- test mat.
- 95% CL:
- 0.542 - 0.739
- Remarks on result:
- other: 4-hr Contact with Undiluted Sample; Rabbit
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 0.373 mL/kg bw
- Based on:
- test mat.
- 95% CL:
- 0.252 - 0.552
- Remarks on result:
- other: 24-hr Contact with Undiluted Sample; Rabbit
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 0.367 mL/kg bw
- Based on:
- test mat.
- 95% CL:
- 0.202 - 0.668
- Remarks on result:
- other: 24-hr Contact with Undiluted Sample; Rabbit
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 2.14 mL/kg bw
- Based on:
- test mat.
- 95% CL:
- 1.45 - 3.17
- Remarks on result:
- other: 24-hr Contact with 20% Aqueous Dilution; Rabbit
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 2.83 mL/kg bw
- Based on:
- test mat.
- 95% CL:
- 1.68 - 4.77
- Remarks on result:
- other: 24-hr Contact with 20% Aqueous Dilution; Rabbit
- Mortality:
- - 4-hour contact period: Deaths occurred at one to 4 days.
- 24-hours contact period: One animal died at 3 hours, but most animals died at one or 2 days. Survivors recovered at 2 days.
- 24-hours contact period with 20% dilution: Deaths occurred at one to 4 days. Survivors appeared normal after 2 days. - Clinical signs:
- other: - 4-hour contact period: Skin reaction included erythema, edema, necrosis, scabs and a few instances of ulceration. One or 2 animals exhibited sluggishness, salivation and prostration. - 24-hours contact period: Irritation of the skin was severe and cons
- Gross pathology:
- - 4-hour contact period: Findings at necropsy included mottled and red lungs, subcutaneous edema (in 2) and one dark red thymus.
- 24-hours contact period: At necropsy, lungs were mottled and red, a few stomachs had multiple black surface foci and a few animals had subcutaneous edema. There were also instances of enlarged kidneys and one spleen with adhesions.
- 24-hours contact period with 20% dilution: Gross pathologic findings included mottled and red lungs, a few enlarged kidneys and subcutaneous edema. - Other findings:
- No data
Any other information on results incl. tables
Body Weight Change Results:
4 hour contact period |
Male |
Female |
1.00 mL/kg |
7 days: N/A |
|
0.50 mL/kg |
7 days: -308 g |
|
0.25 mL/kg |
7 days: -304 to -43 g (mean= -198 g) |
|
1.00 mL/kg |
|
7 days: N/A |
0.71 mL/kg |
|
8 days: -241 g |
0.50 mL/kg |
|
7 days: -381 to -282 (mean= -329 g) |
24 hour contact period |
Male |
Female |
0.80 mL/kg |
7 days: N/A |
|
0.40 mL/kg |
7 days: -488 and -280 g (mean= -384 g) |
|
0.20 mL/kg |
7 days: -207 to -121 g (mean= -167 g) |
|
0.80 mL/kg |
|
7 days: N/A |
0.40 mL/kg |
|
7 days: -403 to -142 g (mean= -307 g) |
0.20 mL/kg |
|
7 days: -405 to -110 g (mean= -222 g) |
0.10 mL/kg |
|
7 days: -121 to 28 g (mean= 48 g) |
24 hour contact period with 20% dilution |
Male |
Female |
4.00 mL/kg |
7 days: N/A |
|
2.00 mL/kg |
7 days: -370 to -270 g (mean= -329 g) |
|
1.00 mL/kg |
8 days: -251 to 108 g (mean= -65 g) |
|
8.00 mL/kg |
|
7 days: N/A |
4.00 mL/kg |
|
7 days: -416 and -302 g (mean= -359 g) |
2.00 mL/kg |
|
7 days: -322 to -184 g (mean= -234 g) |
1.0 mL/kg |
|
7 days: -172 to 50 g (mean= -76) |
Applicant's summary and conclusion
- Interpretation of results:
- Category 3 based on GHS criteria
- Conclusions:
- Percutaneous LD50 = 0.406 mL/kg (Males) and 0.633 mL/kg (Females) for the 4-hr contact with undiluted sample group; Percutaneous LD50 = 0.373 mL/kg (Males) and 0.367 mL/kg (Females) for the 24-hr contact with undiluted sample group; Percutaneous LD50 = 2.14 mL/kg (of dilution, Males) and 2.83 mL/kg (of dilution, Females) for the 24-hr contact with 20% aqueous dilution group.
The test substance was moderately to highly toxic following dermal application for 4 hours or 24 hours. A 20% (v/v) dilution in water was moderately toxic following a 24-hour dermal application. Severe, persistent dermal irritation was evident in each test.
The dermal LD50 value was converted using the density of the test substance : 0.848 g/mL. For males 0.373 mL/kg = 316 mg/kg; for females 0.367 mL/kg = 311 mg/kg
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