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EC number: 200-824-2 | CAS number: 74-95-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP and appropriate OECD testing guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.42 (Skin Sensitisation: Local Lymph Node Assay)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.2600 (Skin Sensitisation)
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Dibromomethane
- EC Number:
- 200-824-2
- EC Name:
- Dibromomethane
- Cas Number:
- 74-95-3
- Molecular formula:
- CH2Br2
- IUPAC Name:
- dibromomethane
- Test material form:
- other: liquid
- Details on test material:
- Certificate of analysis of the test item is attched
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- female
- Details on test animals and environmental conditions:
- At the start of the study the animals were in the weight range of 15 to 23 g, and were eight to twelve weeks old.
The temperature and relative humidity were controlled to remain within target ranges of 19 to 25°C and 30 to 70%, respectively.
Study design: in vivo (LLNA)
- Vehicle:
- acetone/olive oil (4:1 v/v)
- Concentration:
- 50 µL (25 µL per ear) of the undiluted test material
- No. of animals per dose:
- Three groups, each of five animals
- Details on study design:
- Following a preliminary screening test in which no clinical signs of toxicity were noted at a concentration of 100%.
This concentration was selected as the highest dose investigated in the main test of the Local Lymph Node Assay.
Three groups, each of five animals, were treated with 50 µL (25 µL per ear) of the undiluted test material,
or the test material as a solution in acetone/olive oil 4: 1 at concentrations of 50% or 25% v/v.
A further group of five animals was treated with acetone/olive oil 4: 1 alone. - Positive control substance(s):
- hexyl cinnamic aldehyde (CAS No 101-86-0)
Results and discussion
- Positive control results:
- Positive Control Material
Concentration Stimulation Index Result
15% v/v in acetone/olive oile 4:1.....................................4.10..........................................Positive
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: see Remark
- Remarks:
- The Stimulation Indices expressed as the mean radioactive incorporation for each treatment group devided by the mean radioactive incorporation of the vehicle control group are presented: Concentration Stimulation Indiex Result Test Material 25% v/v in acetone/olive oile 4:1.....................................0.70..........................................Negative 25% v/v in acetone/olive oile 4:1.....................................0.70..........................................Negative 50% v/v in acetone/olive oile 4:1.....................................0.63..........................................Negative 100% v/v in acetone/olive oile 4:1...................................1.39..........................................Negative Positive Control Material 15% v/v in acetone/olive oile 4:1.....................................4.10..........................................Positive
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: A table of 'Individual disintegration per minute' is attached in the : "Attached background material" section.
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information
- Conclusions:
- Dibromomehtane is a non sensitiser.
- Executive summary:
Following a preliminary screening test in which no clinical signs of toxicity were noted at a concentration of 100%, this concentration was selected as the highest dose investigated in order to assess the skin sensitisation potential of the test material in the CBA/Ca strain mouse. Three groups, each of five animals, were treated by topical application to the dorsal surface of the ear with 25 µl undiluted test material (99.5% purity, DBM) or the test material as a solution in acetone/olive oil 4:1 at concentrations of 50% or 25% v/v. A further group of five animals was treated with acetone/olive oil 4:1 alone.
The study results demonstrate that the Stimulation Index (SI) expressed as the mean radioactive incorporation for each treatment group divided by the mean radioactive incorporation of the vehicle control group were 1.39, 0.63 and 0.70 for all concentrations of the test material investigated (100%, 50% & 25%, respectively). The positive control group (a concentration of 15% of α-hexylcinnamaldehyde in 4:1 Acetone/Olive oil) showed an acceptable response for a valid study with an SI of 4.10. Hence, it was concluded that the DBM is not skin sensitizer.
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