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Diss Factsheets
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EC number: 203-699-2 | CAS number: 109-73-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Remarks:
- Pre-guideline study performed equivalent to OECD 301 (1981)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Version / remarks:
- 1981
- Deviations:
- no
- Remarks:
- no obvious deviations based on study report
- GLP compliance:
- not specified
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- mixture of sewage, soil and natural water
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
Sludge was taken from 10 different sites in Japan (municipal STPs, industrial STPs, lakes, rivers and bays)
- Laboratory culture: yes
- Method of cultivation: fresh and old sludge was mixed at regular intervals under sufficient aeration;
about 30 minutes after ceasing the aeration the supernatant corresponding to about 1/3 of whole volume was removed.
Then the equal volume of dechlorination water was added to the remaining portion and aerated again, followed by addition
of synthetic sewage* (0.1% w/v). This procedure was repeated once every day. The culturing was carried out at 25 °C.
- Storage conditions: see above
- Storage length: see above
- Preparation of inoculum for exposure: see above
- Pretreatment: see above
- Concentration of sludge: 30 mg/L
* Synthetic sewage: each 5% (w/v) glucose, peptone and monopotassium phosphate were dissolved in dechlorinatded water,
adjusted to pH 7.0 ± 1.0 with sodium hydroxide. - Duration of test (contact time):
- 14 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: basal mineral culture medium (according to the guideline)
- Additional substrate: no
- Solubilising agent (type and concentration if used): not necessary
- Continuous darkness: yes
- Test temperature: 25 °C +/- 1°C- pH: 7.0
- pH adjusted: yes
- Aeration of dilution water: pre-filtered air was used for aeration
- Suspended solids concentration: 30 mg/L
- Replicates for test item samples (with inoculum): n= 2
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes (n=1)
- Abiotic sterile control: yes (n= 2)
- positive control (reference): yes (Aniline), n=1;
TEST SYSTEM
- Culturing apparatus: 300 mL vessels ( Coulometer: Ohkura Electric Co., Ltd)
- Measuring equipment: Closed system oxygen consumption measuring apparatus
- Details of trap for CO2 and volatile organics if used: Soda lime No.1 (extra pure reagent) - Reference substance:
- aniline
- Parameter:
- % degradation (O2 consumption)
- Value:
- 85
- Sampling time:
- 14 d
- Remarks on result:
- other: BOD / ThOD (without NH3 oxidation)
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 66
- Sampling time:
- 14 d
- Remarks on result:
- other: BOD /ThOD (including NH3 oxidation)
- Parameter:
- % degradation (TOC removal)
- Value:
- 98
- Sampling time:
- 14 d
- Parameter:
- other: HPLC analysis
- Value:
- 100
- Sampling time:
- 14 d
- Details on results:
- BOD test item flask A (300 ml; 100 mg/L test item; day 14) = BOD test item flask B (300 ml; 100 mg/L test item) = 68.4 mg;
BOD incoculum control (300 ml; day 14): 1.5 mg
Net-BOD (day 14) = 68.4 mg - 1.5 mg = 66.9 mg
ThOD_NH3: 2.63 mg O2/mg, i.e. 78.9 mg per 30 mg test item;
ThOD_NO2: 3.39 mg O2/mg, i.e. 101.7 mg per 30 mg test item;
% biodegradation (NH3) = 85%
% biodegradation (NO2) = 66%
Aniline:
BOD (day 14; 30 mg) = 69 mg; Net-BOD = 69 mg - 1.5 mg = 67.5 mg;
ThOD_NH3 Aniline: 2.405 mg O2/mg, i.e. 72.15 mg O2 per 30 mg for the test.
ThOD_NO2 Aniline: 3.01 mg O2/mg, i.e. 90.3 mg O2 per 30 mg for the test
% biodegradation (NH3) = 93.5%
% biodegradation (NO2) = 76.4%
10-day window requirement (based on plotted data within the report):
Biodegradation extent after 10 days:
BOD test item flask A (300 ml; 100 mg/L test item; day 14) = BOD test item flask B (300 ml; 100 mg/L test item) = 64.3 mg;
BOD incoculum control (300 ml; day 14): <1.5 mg
Net-BOD (day 14) = 64.3 mg - 1.5 mg = 62.8 mg
% biodegradation (NO2) = 62% - Results with reference substance:
- Within 14 days:
% biodegradation (NH3) = 93.5%
% biodegradation (NO2) = 76.4% - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Based on reliable MITI-test I (OECD 301 C) the test item is readily biodegradable, fulfilling the 10-day window requirement.
- Executive summary:
The test item n-butylamine was tested for ready biodegradability in a reliable study performed according to the MITI-I protocol (equivalent to OECD 301 C, 1981). All relevant data are given in the study report. Biodegradation extent (BOD) reached 85% and 66% without and including nitrification, respectively, within 14 days. The 10 day-window requirement was met (62% biodegradation incl. nitrification within 10 days). All validity criteria (based on OECD 301, 1992) were fulfilled.
Reference
The pass value has been reached in a 10-d window within the 28-d period of the test for all 7 replicates of the test substance.
Description of key information
Based on a reliable MITI-test I (OECD 301 C) the test item is readily biodegradable, fulfilling the 10-day window requirement.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
In the key study (CITI/NITE, 1979), the test item n-butylamine was tested for ready biodegradability in a reliable study performed according to the MITI-I protocol (equivalent to OECD 301 C, 1981). All relevant data are given in the study report. Biodegradation extent (BOD) reached 85% and 66% without and including nitrification, respectively, within 14 days. The 10 day-window requirement was met (62%
biodegradation incl. nitrification within 10 days). All validity criteria (based on OECD 301, 1992) were met.
The following supporting data are available, corroborating the result of the key study:
In a test on inherent biodegradability (Hoechst / Wellens, 1977), n-Butylamine underwent about 90 % and 100% biodegration within 2 and 5 days, respectively, based on determination of COD in the pre-guideline test equivalent to OECD 302B. This result is consitent with ready biodegradability as determined within the key study.
Further, biodegradation of n-butylamine was examined in a pre-guideline study (Yoshimura, 1980) that is comparable to the OECD TG 301-C (MITI-I) test for ready biodegradation. The BOD accounted for more than 50% of the ThOD (theoretical oxygen demand) after 5 days, and for more than 60 % after 12 days of culture.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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