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EC number: 210-765-4 | CAS number: 623-03-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: no guideline reported
- Principles of method if other than guideline:
- Probit analysis (> EC10, EC50) and Williams-Test (> NOEC, LOEC) of data presented in IUCLID entry '623-03-0_4-Chlorbenzonitril_bacteria_cell_inhibition_1990.pdf'.
- GLP compliance:
- no
- Duration:
- 18 h
- Dose descriptor:
- EC10
- Effect conc.:
- 52 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: inhibition of cell multiplication
- Key result
- Duration:
- 18 h
- Dose descriptor:
- EC50
- Effect conc.:
- 138 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: inhibition of cell multiplication
- Duration:
- 18 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 26 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: inhibition of cell multiplication
- Duration:
- 18 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 52 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: inhibition of cell multiplication
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The 18-h EC50 of 4-chlorobenzonitrile for Pseudomonas putida is 138 mg/L.
- Executive summary:
The EC10 and EC50 of 4-chlorobenzonitrile as well as the NOEC and LOEC were calculated by using probit analysis and the Williams-Test, respectively, on the data presented by Coenen, 1990. The following results were presented:
- EC10 (18 h) = 52 mg/L
- EC50 (18 h) = 138 mg/L
- LOEC (18 h) = 52 mg/L
- NOEC (18 h) = 26 mg/L
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Qualifier:
- according to guideline
- Guideline:
- other: TETRATOX-Test
- GLP compliance:
- not specified
- Vehicle:
- yes
- Test organisms (species):
- Tetrahymena pyriformis
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 137 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The 48-h EC50 (basis for effect: growth inhibition) of 4-chlorobenzonitrile for Tetrahymena pyriformis is 137 mg/L.
- Executive summary:
The toxicity of 4-chlorobenzonitrile to the freshwater ciliate Tetrahymena pyriformis was determined in a 48-h TETRATOX-Test. 0.75 % DMSO was used as vehicle. At this concentration DMSO was stated to have no toxic effects on the population growth of Tetrahymena pyriformis.
The 48-h EC50 was determined to be 137 mg/L (nominal concentration).
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: no guideline reported
- Principles of method if other than guideline:
- MicrotoxTM-test; no details reported, secondary reference
- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Vehicle:
- no
- Test organisms (species):
- Vibrio fisheri
- Test type:
- static
- Water media type:
- saltwater
- Total exposure duration:
- 15 min
- Key result
- Duration:
- 15 min
- Dose descriptor:
- EC50
- Effect conc.:
- 8.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: bioluminescence
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 15 min EC50 of 4-chlorobenzonitrile for the bioluminescent marine bacterium Vibrio fisheri is 8.7 mg/L.
- Executive summary:
The EC50 of 4-chlorobenzonitrile was determined in a Microtox-test. The bioluminescent marine bacterium Vibrio fisheri was exposed to the test substance for a total duration of 15 min.
The EC50 was determined to be 8.7 mg/L.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- April 1990
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: Bewertung wassergefährdender Stoffe, III Bestimmung der akuten Bakterientoxizität, LTwS Nr. 20, September 1979 (UBA)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- yes
- Test organisms (species):
- Pseudomonas putida
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 18 h
- Remarks on exposure duration:
- ± 2 h
- Key result
- Duration:
- 18 h
- Dose descriptor:
- other: toxicity threshold
- Effect conc.:
- 50.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxicity threshold value of 4-Chlorobenzonitrile is 50.6 mg/L for Pseudomonas putida. The ‘Bewertungszahl' (Assessment figure or value for bacterio toxicity) is therefore 4.3 (Bewertung wassergefährdender Stoffe, 1979).
- Executive summary:
4-chlorobenzonitrile was investigated for its ability to inhibit the cell multiplication of the bacteria species Pseudomonas putida. Cultures of Pseudomonas putida bacteria were exposed to dilution series of 4-chlorobenzonitrile.
Samples of the 4 -chlorobenzonitrile dilution taken for analysis of the actual concentration, revealed that the bacteria were esposed to concentrations ranging from 206 to 0.16 mg 4-chlorobenzonitrile per liter.
After incubation the extinction values of the bacterial cell suspensions were measured and a toxicity threshold value of 4-chlorobenzonitrile of 50.6 mg/L for Pseudomonas putida was determined. The ‘Bewertungszahl' (Assessment figure or value for bacterio toxicity) is therefore 4.3 (Bewertung wassergefährdender Stoffe, 1979).
Referenceopen allclose all
Description of key information
The most sensitive result for freshwater micororganisms is available for
Tetrahymena pyriformis. The 48-h EC50 was determined to be 137 mg/L (>
key value for freshwater).
The available 15 min EC50 for the marine bioluminiscent bacterium Vibrio
fisheri is 8.7 mg/L (> key value for saltwater).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 137 mg/L
Additional information
4-chlorobenzonitrile was investigated for its ability to inhibit the cell multiplication of the bacteria species Pseudomonas putida. Cultures of Pseudomonas putida bacteria were exposed to dilution series of 4-chlorobenzonitrile.
Samples of the 4-chlorobenzonitrile dilution taken for analysis of the actual concentration, revealed that the bacteria were exposed to concentrations ranging from 206 to 0.16 mg 4-chlorobenzonitrile per liter.
After incubation the extinction values of the bacterial cell suspensions were measured and a toxicity threshold value of 4-chlorobenzonitrile of 50.6 mg/L for Pseudomonas putida was determined. The ‘Bewertungszahl' (Assessment figure or value for bacterio toxicity) is therefore 4.3 (Bewertung wassergefährdender Stoffe, 1979).
The EC10 and EC50 of 4-chlorobenzonitrile as well as the NOEC and LOEC values were calculated by using probit analysis and the Williams-Test, respectively, on the data presented by Coenen, 1990. The following results were presented:
- EC10 (18 h) = 52 mg/L
- EC50 (18 h) = 138 mg/L
- LOEC (18 h) = 52 mg/L
- NOEC (18 h) = 26 mg/L
The EC50 of 4-chlorobenzonitrile was determined in a Microtox-test. The bioluminescent marine bacterium Vibrio fisheri was exposed to the test substance for a total duration of 15 min. The EC50 was determined to be 8.7 mg/L.
The toxicity of 4-chlorobenzonitrile to the freshwater ciliate Tetrahymena pyriformis was determined in a 48-h TETRATOX-Test. 0.75 % DMSO was used as vehicle. At this concentration DMSO was stated to have no toxic effects on the population growth of Tetrahymena pyriformis.
The 48-h EC50 was determined to be 137 mg/L (nominal concentration).
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