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EC number: 204-800-2 | CAS number: 126-73-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Several acute toxicity studies were performed with tributyl phosphate. Here only the reliable studies are reported:
In the key study for acute oral toxicity an LD50 of 1553 mg/kg bw was found in rats. This value was supported by another study in rats were an LD50 of 1400 mg/kg bw was determined.
In the key study for acute dermal toxicity an LD50 > 3100 mg/kg bw (highest applied dose) was observed in rats.
Acute inhalation toxicity was determined with tributyl phosphate in male and female Wistar rats (key-study). The doses tested for 4 hours were 0, 511, 801, 2140 mg/m³, and the highest technically achievable concentration of 4242 mg/m³ air. At 4242 mg/m³ air 2 of 5 male rats died, whereas the female rats in this dose group showed no mortalities. Acute inhalation exposure to tributyl phosphate exerted clinical signs that were indicative of distinct irritation to the respiratory tract. The LD 50 was approx. 4242 mg/m³ air in the more sensitive male rat.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1986
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No OECD guideline or GLP defined.
- Qualifier:
- according to guideline
- Guideline:
- other: EG-Richtlinie 84/449 (Amtsblatt der Europäischen Gemeinschaften 27, 1984, L 251, 96)
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- no
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Borchen, Germany
- Age at study initiation: 9 (males) to 14 (females) weeks
- Weight at study initiation: 165 g for males and 163 g for females
- Fasting period before study: 16 h
- Housing: 5 animals per cage
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +- 2
- Humidity (%): 50 +- 10
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: From: 1986-14-01 To: 1986-02-24 - Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Doses:
- 1.3; 1.5; 1.8; 2,.0; 3.1 mL/kg bw
- No. of animals per sex per dose:
- 5/sex/dose
- Control animals:
- not specified
- Details on study design:
- body weight was determined before application, one week later and at the end of the 14-day observation period
- Statistics:
- after Rosiello et al., J. Tox. Environ. Health 3, 797, 1977
- Dose descriptor:
- LD50
- Effect level:
- 1 552 mg/kg bw
- Mortality:
- 1,5 ml/kg bw: 2 male/ 2 female
1,8 ml/kg bw: 3 male/ 4 female
2,0 ml/kg bw: 4 male/ 4 female
3,1 ml/kg bw: 5 male/ 5 female - Clinical signs:
- other: Clinical signs were observed in dose groups: 1,5; 1,8; 2,0; 3,1 ml/kg bw.
- Gross pathology:
- No findings in dose group 1,3 ml/kg.
In dose group 1,5 ml/kg 4 rats (2 male/2 female) showed effects on gastric mucosa.
In the 1,8 ml/kg dose group 3 male animals showed effects on gastric mucosa and 4 female animals had effects on gastric mucosa, inflated intestine and reddened stomach.
In the 2,0 ml/kg dose group 4 male and 4 female animals showed effects on gastric mucosa, inflated intestines and a reddened lung. In the dose group of 3,1 ml/kg all animals (5male/ 5 female) showed effects on gastric mucosa, inflated intestines and a reddened lung. - Executive summary:
Tributyl phosphate was tested in a single dose experiment in male and female Wistar rats.
The doses tested were: 1,3; 1,5; 1,8; 2,0; 3,1 ml/kg bw.
Signs of intoxication were: diminuated general condition; narcose, bloody eye margins, face down position or lateral position, ruffled fur.
The LD50 was: 1,6 ml/kg bw for male and female rats.
Reference
After a single oral dose of 1,5 up to 3,1 ml/kg bw following symptoms could be observed in the test animals:
Diminished general condition; narcosis, bloody eye margins, face down position or lateral position, ruffled fur.
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 1 553 mg/kg bw
Acute toxicity: via inhalation route
Link to relevant study records
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Qualifier:
- according to guideline
- Guideline:
- other: EG-Richtlinie 84/449/EWG B.2.
- Qualifier:
- according to guideline
- Guideline:
- other: TSCA-Guideline §798.1150
- GLP compliance:
- yes
- Test type:
- traditional method
- Limit test:
- no
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Borchen, Germany
- Age at study initiation: 2-3 months
- Weight at study initiation: 166 to 210 g
- Fasting period before study: none
- Housing: 5 animals per cage
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22+- 2
- Humidity (%): 40-50
- Air changes (per hr): 10
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: 1989 - Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- nose/head only
- Vehicle:
- other: unchanged (no vehicle)
- Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- 0, 511, 801, 2140, and 4242 mg/m³ air
- No. of animals per sex per dose:
- 5/sex/dose
- Control animals:
- yes
- Dose descriptor:
- LC50
- Effect level:
- > 4.242 mg/L air
- Exp. duration:
- 4 h
- Mortality:
- 2 male rats in dose group 4242 mg/L air died.
- Clinical signs:
- other: Group 3 ( 801 mg/L air) showed piloerection, diminuated motility, difficulties in breathing, bloody snout. Group 4 (2140 mg/L air) showed piloerection, untended fur, diminuated motility, nose secretion, sniffing sounds, bradypnoe, complications in breath
- Body weight:
- 3 days after experiment in the male rats of group 2 (511 mg/L air) a retardation in weight gain and in groups 3 to 5 (801; 2140; 4242 mg/m³ air) a loss of weight could be observed.
- Gross pathology:
- died intercurrently: inflated, liver-like lung; red-crusted nose; anemic spleen and kidneys; changed liver signing; gastrointestinal trakt filled with yellow mucus.
Animals killed after observation period: inflated lung; effects in spleen (anemic, reduced in volume, enlarged, dark coloured); changed liver signing; inflated intestine. - Executive summary:
Acute inhalative toxicity was tested with the test substance in male and female Wistar rats.
The doses tested for 4 hours were: 0; 511; 801; 2140; 4242 mg/m³ air.
Observed symptoms: diminuated motility, ataxie, adynamia in hindpaw, prostration, piloerection, untended fur, nose secretion, sniffing sounds, difficulties in breathing, bradypnoe, breathing sounds, chromodacryorrhoe, red-coloured urine, inflated abdomen, bloddy snout, loss of myotactic reflex, reduction of body weight. These symptoms point to a strong adverse effect to the respiratory tract.
After 4242 mg/m³ air 2 of 5 male rats died; the female rats in this dose group showed no mortalities.
The LD 50 was approx. 4242 mg/m³ air in the the more sensitive male rat.
Reference
Acute toxicity: inhalation: aerosol
N | Concentration nomin. analyt. mg/m³ air | Toxicological result | Duration of symptoms | Timepoint od death | Particle < 5 µm (%) | |||
Male rat | ||||||||
1 | air-control | 0* | 0** | 5*** | - | - | - | |
2 | 2425 | 511 | 0 | 0 | 5 | - | - | 100 |
3 | 9700 | 801 | 0 | 5 | 5 | 4h-6h | - | 100 |
4 | 13400 | 2140 | 0 | 5 | 5 | 4h-7h | - | 100 |
5 | 48500 | 4242 | 2 | 5 | 5 | 4h-28d | 2d | 100 |
Female rat | ||||||||
1 | air-control | 0 | 0 | 5 | - | - | - | |
2 | 2425 | 511 | 0 | 0 | 5 | - | - | 100 |
3 | 9700 | 801 | 0 | 5 | 5 | 4h-6h | - | 100 |
4 | 19400 | 2140 | 0 | 5 | 5 | 4h-4d | - | 100 |
5 | 48500 | 4242 | 0 | 5 | 5 | 4h-7d | - | 100 |
LC50 male: approx. 4242 mg/m ³ air.
N= Group number
4h= direktly after exposition
*= Number of mortalities
**= Number of animals with symptoms
***= Number of animals used
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LC50
- Value:
- 4 242 mg/m³ air
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: few experimental details - but sufficient for assessment
- Principles of method if other than guideline:
- undiluted test material was applied to intact, clipped skin (dorsal) of New Zealand albino male and female rabbits to determine the minimum lethal dose by the dermal route. Following application, treated areas were covered with plastic to preclude evaporation of the ester. Test material was washed off after the 24-hr exposure perid. animals were held for a 14-day observation period, after which they were sacrificed and subjected to gross autopsy.
- GLP compliance:
- not specified
- Test type:
- other: no data
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Duration of exposure:
- 24 hours
- Doses:
- no data
- No. of animals per sex per dose:
- no data
- Control animals:
- not required
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 3 100 mg/kg bw
- Remarks on result:
- other: exposure: 24 hours
- Executive summary:
Undiluted test material was applied to intact, clipped skin (dorsal) of New Zealand albino male and female rabbits to determine the minimum lethal dose by the dermal route. Following application, treated areas were covered with plastic to preclude evaporation of the ester. Test material was washed off after the 24-hr exposure perid. animals were held for a 14-day observation period, after which they were sacrificed and subjected to gross autopsy.
LD50 > 3100 mg/kg bw (rabbit)
reference: Johannsen (1977)
Reference
bo data
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 3 100 mg/kg bw
Additional information
Tributyl phoshate was harmful by inhalation and if swallowed. In the key study for acute oral toxicity a LD50 = 1553 mg/kg bw was found. In the key study for acute inhalation toxicity a LC50 = 4242 mg/m³ (mortality 2/5) for male rats and LC50 > 4242 mg/m³ (mortality 0/0) for female rats was determined. By dermal application tributyl phosphate was only slightly toxic.
Justification for classification or non-classification
An LD50 of 1530 (oral, rat, male) was found in a valid study, therefore a classification with Acute Tox 4 (H302) is justified.
For inhalation an LC50 of 4242 mg/m³ (inhalation, rat, male) and LC50 > 4242 mg/m³ (inhalation, rat, female) was determined. 2/10 male rats died at this concentration (highest applied dose). A classification for acute inhalation toxicity is not warranted.
A classification for acute dermal toxicity is not justified based on an LD50 of > 2000 mg/kg bw.
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