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EC number: 212-079-0 | CAS number: 760-23-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Repeated dose toxicity: oral
Administrative data
- Endpoint:
- short-term repeated dose toxicity: oral
- Remarks:
- combined repeated dose and reproduction / developmental screening
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-guideline study. Adopted according to OECD SIDS (public available peer reviewed source). The original source is available and has been reviewed.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Toxicity testing reports of environmental chemicals
- Author:
- Ministry of Health & Welfare (Japan)
- Year:
- 1 996
- Bibliographic source:
- Vol. 4, chapter: 3,4-Dichloro-1-butene, pp. 529-533, ISSN 1340-3842
- Reference Type:
- secondary source
- Title:
- SIDS Initial Assessment Report for 11th SIAM
- Author:
- OECD SIDS
- Year:
- 2 003
- Bibliographic source:
- UNEP Publications
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 422 (Combined Repeated Dose Toxicity Study with the Reproduction / Developmental Toxicity Screening Test)
- GLP compliance:
- yes
- Limit test:
- no
Test material
- Reference substance name:
- 3,4-dichlorobut-1-ene
- EC Number:
- 212-079-0
- EC Name:
- 3,4-dichlorobut-1-ene
- Cas Number:
- 760-23-6
- Molecular formula:
- C4H6Cl2
- IUPAC Name:
- 3,4-dichlorobut-1-ene
- Details on test material:
- - Name of test material (as cited in study report): 3,4-Dichloro-1-butene
- Analytical purity: 99.7%
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Crj:CD (SD)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 9 weeks old for males, 8 weeks old for females
- Weight at study initiation: 343-384 g for males, 192-222 g for females
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: sesame oil
- Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- males: 44 days; females: from 14 days before mating to day 3 of lactation
- Frequency of treatment:
- daily
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0.4, 2, 10 or 50 mg/kg bw
Basis:
actual ingested
- No. of animals per sex per dose:
- 10
- Control animals:
- yes, concurrent vehicle
- Details on study design:
- Post-exposure period: no data
- Positive control:
- No
Examinations
- Observations and examinations performed and frequency:
- CAGE SIDE OBSERVATIONS: Yes
DETAILED CLINICAL OBSERVATIONS: Yes
General condition was observed once a day.
BODY WEIGHT: Yes
- Time schedule for examinations: once a week.
HAEMATOLOGY: Yes
- Time schedule for collection of blood: for males was carried out only at time of necropsy after 44 days of chemical exposure.
CLINICAL CHEMISTRY: Yes
- Time schedule for collection of blood: for males was carried out only at time of necropsy after 44 days of chemical exposure.
URINALYSIS: Yes
- Time schedule for collection of urine: Urinalysis was carried out 38 or 40 days (males)
Food/water consumption were determined once a week. - Sacrifice and pathology:
- ORGANS EXAMINED AT NECROPSY:
-organ weight: brain, heart, liver, kidney, spleen, adrenal, thymus, testes, epididymis
-microscopic: all animals in control and 50 mg/kg, and males failed to cause pregnancy or non-pregnant females: brain, pituitary gland, eyeball, thyroid gland, parathyroid gland, thymus, heart, lung, liver, kidney, adrenal, spleen, stomach, small intestine, large intestine, pancreas, urinary bladder, bone marrow, testes, epididymis, prostate, seminal vesicle, ovary, uterus, vagina, mammary gland. - Statistics:
- Performed but not reported in detail.
Results and discussion
Results of examinations
- Clinical signs:
- effects observed, treatment-related
- Description (incidence and severity):
- 1 female was dying 2 days after parturition.
- Mortality:
- mortality observed, treatment-related
- Description (incidence):
- 1 female was dying 2 days after parturition.
- Body weight and weight changes:
- no effects observed
- Description (incidence and severity):
- Significant differences from controls were not observed
- Food consumption and compound intake (if feeding study):
- not examined
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- not examined
- Ophthalmological findings:
- not examined
- Haematological findings:
- effects observed, treatment-related
- Description (incidence and severity):
- increase in total protein
- Clinical biochemistry findings:
- effects observed, treatment-related
- Urinalysis findings:
- effects observed, treatment-related
- Behaviour (functional findings):
- not examined
- Organ weight findings including organ / body weight ratios:
- effects observed, treatment-related
- Description (incidence and severity):
- Male: increase in kidney weight at 10 mg/kg (absolute (p<0.05) and liver weight at 50 mg/kg (absolute (p<0.01) and relative (p<0.01)); female: increase in kidney weight at 50 mg/kg (p<0.01)).
- Gross pathological findings:
- effects observed, treatment-related
- Description (incidence and severity):
- Liver swelling (male: 2/10, female: 1/10) and kidney swelling (male: 3/10, female: 0/10) at 50 mg/kg.
- Histopathological findings: non-neoplastic:
- effects observed, treatment-related
- Description (incidence and severity):
- male and female showed hepatocellular hypertrophy at 50 mg/kg
- Histopathological findings: neoplastic:
- no effects observed
- Details on results:
- Males: Ephemeral decreased locomotor activity (all rats, the first day of the study in the 50 mg/kg bw/day group, 1/10, day 5-12) and ephemeral slaver (50 mg/kg: 10/10, the first day of the study).
Females: Death(50 mg/kg: 1/10), ephemeral decreased locomotor activity (50 mg/kg: 10/10), and ephemeral slaver (50 mg/kg: 5/10, the first day of the study).
In males, absolute kidney weights were slightly increased with 10 mg/kg/day dose. Absolute and relative weights of the liver and kidneys were increased with 50 mg/kg/day dose. Blood chemical examination revealed an increase in total protein. The histopathological examination revealed increased hyaline droplets in the renal tubular epithelium with doses of 10 and 50 mg/kg/day and hepatocellular hypertrophy with dose of 50 mg/kg/day.
One female was sacrificed in a moribund condition on day 2 of lactation. An increase in relative kidney weights was observed at the dose of 50 mg/kg/day. However, no histopathological changes considered to be related to the change of the kidney weight were detected. Hepatocellular hypertrophy was observed at the dose of 50 mg/kg/day.
NOAELs in this repeat dose study are 2 mg/kg/day for males and 10 mg/kg/day for females, but the renal toxicity in males is considered to be male rat specific, probably due to alpha2U-globulin involvement. Therefore, the NOAEL for repeated dose toxicity is considered to be 10 mg/kg/day.
Effect levels
open allclose all
- Dose descriptor:
- NOAEL
- Effect level:
- 10 mg/kg bw/day (actual dose received)
- Based on:
- test mat.
- Sex:
- male/female
- Dose descriptor:
- LOAEL
- Effect level:
- 50 mg/kg bw/day (actual dose received)
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- other: Based on ephameral decreased locomotor activity, liver swelling, kidney swelling, liver weight, in male and female animals.
Target system / organ toxicity
- Critical effects observed:
- not specified
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.