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EC number: 202-445-8 | CAS number: 95-73-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to reproduction
Administrative data
- Endpoint:
- one-generation reproductive toxicity
- Remarks:
- based on generations indicated in Effect levels (migrated information)
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 422 (Combined Repeated Dose Toxicity Study with the Reproduction / Developmental Toxicity Screening Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Benzene, 2,4-dichloro-1-methyl-
- IUPAC Name:
- Benzene, 2,4-dichloro-1-methyl-
- Reference substance name:
- 2,4-dichlorotoluene
- EC Number:
- 202-445-8
- EC Name:
- 2,4-dichlorotoluene
- Cas Number:
- 95-73-8
- Molecular formula:
- C7H6Cl2
- IUPAC Name:
- 2,4-dichloro-1-methylbenzene
- Details on test material:
- Purity: 98,96 %
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- Crj: CD(SD)
- Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Duration of treatment / exposure:
- Male: 46 days,
female: from 14 days before mating to day 3 of lactation
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0, 12.5, 79, 500 mg/kg/day
Basis:
nominal in water
Results and discussion
Results: P0 (first parental generation)
General toxicity (P0)
- Clinical signs:
- effects observed, treatment-related
- Description (incidence and severity):
- Increased salivation occurred in the group with 500 mg/kg bw/day. This effect was observed only in females.
- Organ weight findings including organ / body weight ratios:
- effects observed, treatment-related
Reproductive function / performance (P0)
- Reproductive performance:
- effects observed, treatment-related
- Description (incidence and severity):
- Group with 500 mg/kg bw/day: Only 5/12 females became pregnant. It is unclear whether the decreased fertility is caused by the male or the female.
Effect levels (P0)
open allclose all
- Dose descriptor:
- NOEL
- Effect level:
- 12.5 mg/kg bw/day
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- other: repeated dose toxicity
- Dose descriptor:
- NOEL
- Effect level:
- 79 mg/kg bw/day
- Based on:
- test mat.
- Sex:
- male/female
- Basis for effect level:
- other: Basis for effect: fertility
- Dose descriptor:
- NOEL
- Effect level:
- 79 mg/kg bw/day (nominal)
- Based on:
- test mat.
- Sex:
- female
- Basis for effect level:
- other: Basis for effect: maternal
Results: F1 generation
General toxicity (F1)
- Body weight and weight changes:
- effects observed, treatment-related
- Description (incidence and severity):
- Bodyweight of the offspring was reduced on the day of the birth and on the fourth day after the birth (group with 500 mg/kg/day)
Effect levels (F1)
- Dose descriptor:
- NOEL
- Generation:
- F1
- Effect level:
- 500 mg/kg bw/day (nominal)
- Based on:
- test mat.
- Basis for effect level:
- other: Basis for effect: Bodyweight of the offspring
Overall reproductive toxicity
- Reproductive effects observed:
- not specified
Applicant's summary and conclusion
- Conclusions:
- The NOEL for reproductive/ developmental toxicity for both sexes was considered to be 79 mg/kg/day.
- Executive summary:
2,4-Dichlorotoluene was studied for oral toxicity in rats according to the OECD combined repeated dose and reproductive/developmental toxicity test [OECD TG 422] at doses of 0, 12.5, 79, 500 mg/kg/day.
Although this combined study was designed to investigate reproductive capability in parental generation as well as development in F1offspring, parameters to evaluate developmental toxicity were limited to only body weights at day 0 and day 4 after birth, and autopsy findings at day 4. Regarding reproductive ability, all pairs in the 12.5 and 79 mg/kg groups achieved pregnancy. In the 500 mg/kg group, 12 pairs showed evidence of copulation with a sperm positive vaginal smear, however, only 5 pairs out of them achieved pregnancy. In six non-pregnant pairs in the 500 mg/kg group, vaginal plugs were not noted or a few sperm were found in the vaginal smears. This result suggests that the male reproductive organs and secondary reproductive organs had functional disorders. Regarding body weight changes of pups, decreases in liver and body weights were noted in the 500 mg/kg group on day 1 of lactation. For delivery or lactating behaviour of dams, viability, general appearance or autopsy of pups, no effects related to the administration of this chemical was noted. On the basis of above-described effects, the NOEL for reproductive/ developmental toxicity for both sexes was considered to be 79 mg/kg/day.
This was a combined repeated dose and reproductive/developmental toxicity test, see also section 7.5.1.
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