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EC number: 293-316-5 | CAS number: 91053-50-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Details on test solutions:
- The limit test concentration was prepared by direct addition of the test substance into the 10-L test chambers. Test substance was weighed onto weigh paper, which was rinsed with Haskell well water (HWW) and then placed into tank to soak. Periodically, tweezers were used to pick up the weight paper and rinse with HWW until all of the test substance was rinsed into the tank. The test solutions were stirred for approximately 63 minutes. The limit test concentration was very cloudy with no visible precipitate present and bubbles on the surface.
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Age at study initiation: juvenile
- Feeding during test: No
ACCLIMATION PERIOD: 2 days
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 100-140 mg/L
- Test temperature:
- 11.9 - 12.1 °C
- pH:
- 7.9 to 8.3
- Dissolved oxygen:
- 6.4 to 10.9 mg/L
- Nominal and measured concentrations:
- A nominal limit test concentration of 100 mg/L
- Details on test conditions:
- The nominal limit test concentration of 100 mg/L of test substance was chosen for the definitive limit test based on the results of a preliminary range-finding study.
Test chambers were stainless steel aquaria [30 (length) x 30 (width) x 30 (height) cm] which held approximately 10 L of test solution (26-L maximum volume; 11.5-cm test solution depth). One dilution water control test chamber and 1 limit test concentration chamber with 7 fish in each chamber were used for testing (total of 7 fish in the control and 7 fish in the limit test concentration). Each chamber was covered with a glass plate to prevent fish from escaping. Random numbers were used to assign the position of test chambers in the waterbath.
Rainbow trout used in this study were not fed approximately 71.5 hours prior to nor during the test, and were assigned to the test chambers using random numbers. Mortality and behavioural observations were made at test start, every 24 hours thereafter, and at test end. Criteria for death were the absence of opercular movement and lack of reaction to gentle prodding.
A recirculating waterbath was used to maintain mean temperature in the test chambers during the 96-hour test at approximately 12.0ºC with a range of 11.9 to 12.1ºC. In addition, a continuously-recording thermometer was used to check temperature variations in the waterbath. A photoperiod of 16 hours light (approximately 338-362 Lux) and 8 hours darkness was employed, which included 30 minutes of transitional light (121-154 Lux) preceding and following the 16-hour light interval. Test solutions were not aerated during the test and were disposed of in an appropriate manner at test end. - Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Details on results:
- At test conclusion, fish from the dilution water control ranged from 2.6 to 3.1 cm in standard length (mean 3.0 cm) and 0.180 to 0.327 g in wet weight, blotted dry (mean 0.261 g). Standard length of the longest fish was not more than twice the length of the shortest fish in the control. Loading in the water control was 0.183 g/L at test conclusion. No mortality or sublethal effects were seen in the dilution water or in the 100 mg/L nominal test substance limit test concentration at the end of 96 hours.
- Sublethal observations / clinical signs:
Table 1: Mortality and Sublethal Effects of Rainbow Trout in Unaerated Static Acute, 96 -Hour Limit Test
Nominal Concentration (mg/L)
Number Dead /Number at Study Start
24 Hours
48 Hours
72 Hours
96 Hours
Dilution Water Control
0/7
0/7
0/7
0/7
100
0/7
0/7
0/7
0/7
- Validity criteria fulfilled:
- yes
- Conclusions:
- 96-h LC50 > 100 mg/L
- Executive summary:
The acute toxicity of the test substance to unfed fingerling rainbow trout, Oncorhynchus mykiss, was determined in an unaerated, static, acute, 96-hour limit test. The study was conducted with a 100 mg/L nominal concentration of the test substance and a dilution water control at a mean temperature of 12.0 ºC (range of 11.9-12.1 ºC). A dilution water control test chamber and a limit test concentration chamber with 7 fish in each chamber were used for testing (total of 7 fish in the control and in the limit test concentration). Fish in the dilution water control ranged from 2.6 to 3.1 cm in standard length (mean 3.0 cm), and 0.180 to 0.327 g in wet weight, blotted dry (mean 0.261 g) at test end. Control loading at test end was 0.183 g/L. No mortality or sublethal effects were seen at the 100 mg/L nominal limit test concentration of the test substance or in the control at the end of the 96-hour limit test. The 96-hour LC50, based on the nominal limit test concentration of the test substance and mortality, was greater than 100 mg/L.
Reference
Description of key information
96-hour LC50>100 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
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