Carbonic acid, ethyl methyl ester

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Feb 13 - Feb 15, 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

The study was performed in compliance with the Good Laboratory Practice (GLP) regulations (revised in 1997, ENV/MC/CHEM(98)17). The method followed that described in the OECD Guidelines for Testing of Chemicals (Adopted: 4 April 1984) No 202 "Daphnia sp., Acute Immobilisation Test and Reproduction Test".

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Principles of method if other than guideline:

None

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

433-480-9

EC Name:

-

Cas Number:

623-53-0

Molecular formula:

C4H8O3

IUPAC Name:

ethyl methyl carbonate

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Sampling method: A volume of test sample was diluted with acetone to give a final theoretical concentration of 20 mg/l.
- Sample storage conditions before analysis: n.a.

Test solutions

Vehicle:

no

Details on test solutions:

i) Stock Solutions
a) CaC12.2H20 11.76 g/l
b) MgSO4.7H20 4.93 gll
c) NaHC03 2.59 g/l
d) KCI 0.23 g/l

For preparation, an aliquot (25 ml) of each of solutions a-d was added to each litre (final volume) of deionised water .
After preparation, the reconstituded water was aerated until the dissolved oxygen concentration was approximately air-saturation value.

- Hardness: about 14° dH (about 250 mg/L CaCO3)
- pH: 7.8 ± 0.2, after an aeration for 24 hours.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Adult Daphnia magna were maintained in polypropylene vessels containing approximately 2 litres of reconstituted water in a temperature controlled room at 21°C. The lighting cycle was controlled to give a 16 hours light and 8 hours darkness cycle with 20 minute dawn and dusk transition periods. Each culture was fed daily with a suspension of algae (Chlarella sp.). Culture conditions ensured that reproduction was by parthenogenesis. Gravid adults were isolated the day before initiation of the test, such that the young daphnids produced overnight were less than 24 hours old. These young were removed from the cultures and used for testing. The diet and diluent water are considered not to contain any contaminant that would affect the integrity or outcome of the study.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

14 ° dH (about 250 mg/L CaCO3)

Test temperature:

21-22 °C

pH:

7.8 +/- 0.2

Dissolved oxygen:

at least 87 %

Salinity:

see test solution details

Nominal and measured concentrations:

measured 100 mg/L

Details on test conditions:

1) Range finding test

Dose level: 0.1, 1, 10, 100 mg/L
Number of daphnids: 40 (4 x 10)
Vessel: 300 mL glass vessel
Volume: 300 mL reconstituted water
Temperature: 21 °C
Photoperiod: 16 hours light and 8 hours darkness
Exposure. 48 h
Food during exposure: No
Aearation during exposure: no
Observation period: 24 and 48 hours



2) Main study

Dose level: 100 mg/L
Replicates: 4
Number of daphnids: 40 (4 x 10)
Vessel: 300 mL glass vessel
Volume: 300 mL reconstituted water
Temperature: 21 °C
Photoperiod: 16 hours light and 8 hours darkness
Exposure. 48 h
Food during exposure: No
Aearation during exposure: no
Observation period: 24 and 48 hours
GC Analysis: 0, 48 hours

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 4

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

There was no immobilisation in 40 daphnids exposed to a test concentration of 100 mg/l for a period .

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of the test material to the freshwater invertebrate Daphnia magna has been investigated and gave a 48-Hour EC50 of greater than 100 mg/l. Correspondingly the No Observed Effect Concentration was 100 mg/l.

Executive summary:

Purpose
The purpose of this assay was to identify the aquatic toxicity potential of the test material in Daphnia magna to provide a rational basis for hazard estimation for the test item in aquatic environments

Study Design
Following a preliminary range-funding study, forty daphnids (4 replicates of 10 animals) were exposed to an aqueous solution of the test material at a concentration of 100 mg/l for 48 hours under static test conditions. Immobilisation and any adverse reactions to exposure were recorded after 24 and 48 hours.

Results
The 24 and 48-hour EC50 for the test material to Daphnia magna based on nominal test concentrations was greater than 100 mg/l and correspondingly the No Observed Effect Concentration was 100 mg/l at 24 and 48 hours.

Nominal concentration	Immobilized/exposed daphnids
[mg/L]	24 hours	48 hours
100	0/40	0/40

Analysis of the test solutions at 0 and 48 hours showed measured test concentrations to be near nominal and so the results are based on nominal test concentrations only.

24 h EC50 > nominal 100 mg/L
48 h EC50 > nominal 100 mg/L
24 h NOEC > nominal 100 mg/L
48 h NOEC > nominal 100 mg/L

Conclusion
The acute toxicity of the test material to the freshwater invertebrate Daphnia magna has been investigated and gave a 24- and 48-hour EC50 of greater than 100 mg/l. Correspondingly the No Observed Effect Concentration was 100 mg/l at 24 and 48 hours.