N-methylformamide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions (exposure concentrations in the test and the stability of the test substance were not confirmed by analysis)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Directive 79/831/EEC, Annex V, Part C

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

N-methylformamide

EC Number:

204-624-6

EC Name:

N-methylformamide

Cas Number:

123-39-7

Molecular formula:

C2H5NO

IUPAC Name:

N-methylformamide

Details on test material:

- Name of test material (as cited in study report): Monomethylformamide
- Analytical purity: 99.5 %

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: obtained from Institute National de Recherche Chimique Appliquee, France. Since 1978 maintained in the laboratory of ecology of BASF AG
- Age at study initiation (mean and range, SD): < 24 h
- Feeding during test: no food

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.7 ±0.5 mmol/L

Test temperature:

21.5 °C

pH:

8.0 - 8.3

Dissolved oxygen:

8.4 - 9.2 mg/L

Nominal and measured concentrations:

Test concentrations: 62.5, 125, 250 and 500 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 20 mL glass beakers filled with 10 mL test solution
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic water according to DIN 38412
- Hardness: 2.7 ± 0.5 mmol/L
- Ca/Mg ratio: 4:1
- Conductivity: 600 - 700 µS/cm
- Culture medium different from test medium:
- Intervals of water quality measurement:


OTHER TEST CONDITIONS
- Photoperiod: 12 hours darkness and 12 hours light
- Light intensity: 5 - 6 µE/(m2) wavelength 400 - 700 nm

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Effect values after 24 h:

EC0    = 500 mg/L
EC50   > 500 mg/L
EC100  > 500 mg/L

(values related to nominal concentrations)