Diethyl 1,2-cyclopentanedicarboxylate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994-12-12 to 1994-12-16

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

31st July 1992

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Diethyl 1,2-cyclopentanedicarboxylate

EC Number:

291-751-5

EC Name:

Diethyl 1,2-cyclopentanedicarboxylate

Cas Number:

90474-13-8

Molecular formula:

C11H18O4

IUPAC Name:

1,2-diethyl cyclopentane-1,2-dicarboxylate

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): 852 DIESTER
- Physical state: liquid
- Analytical purity: 96.8 %
- Purity test date: 1994-11-18
- Lot/batch No.: 878601/007 007/95
- Expiration date of the lot/batch: 1996-10-13
- Storage condition of test material: cool and away from light

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: the test substance was dissolved directly in water at 500 mg/l abd agitated mechanically for around 24 hours in an ttempt to completely dissolve it. Test solutions were prepared by further dilution of the stock solution with water to provide a geometric series of concentrations: 0, 1, 10 and 100 mg/l

- Sampling method and sample storage conditions before analysis: a 4 ml sample was taken from the limit test solution and the dilution water at the beginning of the limit test and frozen, and a further 4 ml sample was taken from the limit vessel containing fish and the control vessel at the end of the test. A sample of 4 ml was taken on 5 occasions during the test (T0, T24, T48, T72, T96) from the 10 mg/l (nominal) test solution and was analysed to verify the concentrations of test substancz throughout the test.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Source: "Labbas", Le Hamet, F-27400 Acquigny (France)
- Length at study initiation (length definition, mean, range and SD): average: 46.4 mm, ranging from 40 to 56 mm (based on a measurement of a sample of 30 fishes used in the test)
- Weight at study initiation (mean and range, SD): the mean weight based on a sample of 10 fishes removed at random from the acclimatation tank was 1.7 g
- Feeding during test: no

ACCLIMATION
- Acclimation period: animals were acclimated for 7 days in a flow-through system in water of the same quality and temperature as that to be used in the test.
- Type and amount of food, feeding frequency: during acclimatation, animals were fed trout chow twice daily, ceasing 24 hours before the start of the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

between 137 and 145 mg/l as CaCO3

Test temperature:

between 14.8 and 16.2°C

pH:

between 7.51 and 8.06

Dissolved oxygen:

between 5.9 and 9.5 mg/l (after correction for barometric solution)

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 - < 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

ca. 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The test substance 852 DIESTER is toxic to Oncorhynchus mykiss in a static test at the nominal concentration of 100 mg/l but non toxic at the measured concentration of 7.23 mg/l.
Although the maximum concentration which did not result in mortality was measured as 7.23 mg/l (geometric mean), it is considered unlikemy that serious toxic effects would have been observed at 10 mg/l had it been possible to maintain this concentration.
The test substance 852 DIESTER should not be classified.