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EC number: 218-871-2 | CAS number: 2269-22-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- publication
- Title:
- Identification of Metal Allergens in the Local Lymph Node Assay.
- Author:
- Basketter et al.
- Year:
- 1 999
- Bibliographic source:
- Am J Contact Dermatitis 1999; 10(4): 207-212.
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Deviations:
- yes
- Remarks:
- :
- GLP compliance:
- no
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Aluminium chloride hexahydrate
- EC Number:
- 917-806-1
- IUPAC Name:
- Aluminium chloride hexahydrate
- Details on test material:
- - Name of test material (as cited in study report): AlCl3.6H2O
- Analytical purity: no data
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- not specified
- Details on test animals and environmental conditions:
- Species and strain: mouse, 4 CBA/Ca
Source: Harlan Olac, Bicester, UK.
Justification of species and strain: Mouse is the preferred animal for this test (OECD TG#429)
Sex: not stated
Body weight range at the beginning of the study: not stated
Age at testing: 7 to 12 weeks
No further details mentioned
Study design: in vivo (LLNA)
- Vehicle:
- other: petrolatum
- Concentration:
- 5.0%, 10.0%, and 25.0%
- No. of animals per dose:
- 4
- Details on study design:
- Two days after the last exposure, the mice were injected with 250 µL of phosphate buffered saline (PBS) containing 20 µCi of tritiated thymidine (from Amersham International, Amersham, UK). Five hours later, the mice were killed, draining lymph nodes excised and a single-cell suspension of the pooled lymph node samples was prepared.
The lymph node cell suspension was washed in excess PBS, precipitated with 5% trichloroacetic acid (TCA) at 4 ºC for 18 hours. After resuspension in TCA, β-scintillation counting was used to measure tritium incorporation.
The method used to kill the animals was not reported.
Observations:
Proliferation of cells in the lymph nodes.
- A positive result was defined as a threefold or greater proliferation than in the concurrent vehicle treated controls. - Positive control substance(s):
- other: positive results were seen for metal salts of gold, beryllium, cobalt, mercury, platinum and tin that were tested in parallel
- Statistics:
- NA
Results and discussion
- Positive control results:
- positive responses were noted
In vivo (LLNA)
Resultsopen allclose all
- Key result
- Parameter:
- SI
- Value:
- >= 0.7 - <= 0.8
- Test group / Remarks:
- 5% 0.8; 10% 0.8; 25% 0.7
- Parameter:
- EC3
- Remarks on result:
- not determinable
- Remarks:
- all values SI values < 3
Any other information on results incl. tables
The results for aluminium chloride hexahydrate were negative. There was no information on the irritancy of the tested concentration from a pre-test
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Aluminium chloride hexahydrate did not show sensitizing potential under the conditions used in this assay.
- Executive summary:
Basketter et al. (1999) investigated the allergenic potential of 13 metal salts including aluminium chloride hexahydrate (99% purity) in the Local Lymph Node Assay (LLNA). Groups of 4 CBA/Ca mice (7 to 12 weeks of age) were treated with 25 μL of substance or with an equal volume of the vehicle alone, on the dorsum of both ears. The mice were treated once daily for 3 days. Two days later, the mice were injected with 250 μL of phosphate buffered saline (PBS) with 20 μCi of tritiated thymidine (2 Ci mmol-l). The mice were killed 5 hours later and a single-cell suspension of lymph node cells was prepared by mechanical disaggregation. A substance was considered a skin sensitizer the proliferation in the lymph nodes of treated mice was 3-fold or greater than that in the concurrent vehicle-treated controls. Aluminium chloride hexahydrate administered in petrolatum (vehicle) at test concentrations of 5.0%, 10.0% and 25.0% did not induce a lymph node proliferation response compared to concurrent vehicle-treated controls, and therefore the response was judged as negative. The results of this study add to the weight of evidence for a low sensitisation potential of aluminium 3+ cations.
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