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Diss Factsheets
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EC number: 203-376-6 | CAS number: 106-23-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to reproduction: other studies
Administrative data
- Endpoint:
- toxicity to reproduction: other studies
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study meets basic scientific principles, well documented
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- publication
- Title:
- Assessment of estrogenic activity in some common essential oil constituents
- Author:
- Howes M.-J.R., Houghton P.J., Barlow D.J., Pocock V.J., Milligan S.R.
- Year:
- 2 002
- Bibliographic source:
- J. Pharm. Pharmacol. 54, 1521-1528
Materials and methods
- Principles of method if other than guideline:
- Uterotrophic assay: uterus weights as indicator of estrogenic action after repeated exposure
Acute assay: vascular permeability of uterus as acute indicator of estrogenic action - GLP compliance:
- not specified
- Type of method:
- in vivo
Test material
- Reference substance name:
- Citral
- EC Number:
- 226-394-6
- EC Name:
- Citral
- Cas Number:
- 5392-40-5
- Molecular formula:
- C10H16O
- IUPAC Name:
- Reaction mass of (E)-3,7-dimethylocta-2,6-dienal and (Z)-3,7-dimethylocta-2,6-dienal
- Reference substance name:
- (Z)-3,7-dimethylocta-2,6-dienal
- EC Number:
- 203-379-2
- EC Name:
- (Z)-3,7-dimethylocta-2,6-dienal
- Details on test material:
- - Name of test material (as cited in study report): Citral
- Analytical purity: 95%
Constituent 1
Constituent 2
Test animals
- Species:
- mouse
- Strain:
- Swiss
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: A. Tuck & Son Ltd., Essex, UK
- Age at study initiation: 3 mo
- Weight at study initiation: 25-35 g
- Fasting period before study:
- Diet: ad libitum
- Pretreatment: ovariectomy prerformed under anesthesia at least two weeks before the start of each experiment
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21 +- 1
- Humidity (%): no data
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- dermal
- Vehicle:
- ethanol
- Details on exposure:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): acute assay 100 µl; uterotrophic assay 50 µl
- Concentration (if solution): 1.9 M
VEHICLE
- Justification for use and choice of vehicle (if other than water): ethanol due to solubility
USE OF RESTRAINERS FOR PREVENTING INGESTION: no data - Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- Acute assay of vascular responses: 4 h
Uterotrophic assay: 3 d - Frequency of treatment:
- Acute assay: single
Uterotrophic assay: twice daily - Duration of test:
- Acute assay: 4 h
Uterotrophic assay: sacrifice 12 h after last application
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
950 mg/kg bw
Basis:
other: calculated from dermal dose of 100 µl in the acute assay
- Remarks:
- Doses / Concentrations:
950 mg/kg bw/d
Basis:
other: calculated from two daily dermal doses of 50 µl in the uterotrophic assay
- No. of animals per sex per dose:
- 5
- Control animals:
- yes, concurrent vehicle
- other: positive control: 17ß-estradiol
- Details on study design:
- Acute assay of vascular responses:
4 hrs after the dermal application of the test substance, 50 μl 0.5 μCi [ 125 I]-labeled human serum albumin was injected into the jugular vein of mice anesthetised with tribromoethanol. Thirty minutes later, a blood sample was drawn from the suborbital canthal sinus with a heparinized capillary pipette and the animals were killed by cervical dislocation. The uteri and a sample of thigh muscle were removed, briefly washed in saline, and weighed. The radioactivity in the uterus, plasma sample, and muscle was determined. The tissue-specific extravascular albumin volume (EAV) was expressed as a ratio of the [ 125 I] counts per minute per milligram of tissue to [ 125 I] counts per minute per microliter of plasma and used as an index of tissue vascular permeability.
Uterotrophic assay:
12 hrs after the last dermal application, the mice were killed and the uterine horns removed, blotted and weighed. - Statistics:
- Analysis of variance
Results and discussion
Any other information on results incl. tables
Table: Uterine weight (12 hrs after last application) and vascular permeability 4 hrs following transdermal application
Treatment |
Uterine weight (mg) |
Uterine vascular permeability (EAV) |
Muscle vascular permeability (EAV) |
Citral |
12.5 ± 0.88 |
12.5 ± 4.31 |
0.95 ±- 0.04 |
Vehicle control |
13.5 ± 0.28 |
5.96 ± 0.54 |
1.24 ± 0.04 |
Positive control |
19.0 ± 0.15 * |
20.3 ± 1.12 * |
0.94 ± 0.03 |
significant change compared to vehicle control: * p<0.01
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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