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EC number: 202-086-7 | CAS number: 91-64-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- other company data
- Title:
- Unnamed
- Year:
- 1 993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Coumarin
- EC Number:
- 202-086-7
- EC Name:
- Coumarin
- Cas Number:
- 91-64-5
- Molecular formula:
- C9H6O2
- IUPAC Name:
- 2H-chromen-2-one
- Test material form:
- other: solution
- Details on test material:
- -Material Source: Factory of Saint Fons Chemistry
-Substance type: pure substance
-Lot number 93 027 01
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Details on test solutions:
- concentration tested (mg/l):463; 215; 100; 46.3; 21.5 and 10
Test organisms
- Test organisms (species):
- activated sludge
Study design
- Test type:
- static
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20 degree
- pH:
- 8.0
- Nominal and measured concentrations:
- concentration tested (mg/l):463; 215; 100; 46.3; 21.5 and 10(nominal)
- Details on test conditions:
- Temperature: 20 ℃
Aeration: Sintered n 1.
Flow: 50 l / h controlled
Test pictures containers test tubes 70 mm, round bottom
Total volume test: 500ml
For conditioning of sludge: the sludge is introduced into the test tube and placed under aeration 15 minutes before the introduction of the
product to the study. Their concentration in test is 1.5 g / l of dry matter
Cadence startup tests: Every 15 minutes
Time of sludge / product contact: 30 minutes and 3 hours.
The oxygen consumption speed is measured on mixtures transferred cells measure tight.
Concentrations tested: a range of concentrations geometric progression ∛5 is obtained by means of mother solution at 1 g / l in water redistilled
463, 215, 100, 46.3, 21.5 and 10 mg / l
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- 640 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- See "Any other information on results incl. tables
Any other information on results incl. tables
3- RESULTATS | ||||||||
Concentration en coumarine en mg/L |
blanc 463 |
463 | 215 | 100 | 46, 3 | 21, 5 | 10 | témoin 0 |
incubation 30 mn | ||||||||
Activité respì-ratoire mg O2 . l/mn |
0 | 0,412 | 0,515 | 0,604 | 0,651 | 0,666 | 0,692 | 0,637 |
inhibition% -30 m n |
35 | 19 | 5 | -2 | -5 | -9 | / | |
incubation 3 h | ||||||||
Activité respì-ratoire mg O2 . l/mn |
0 | 0,372 | 0,478 | 0,552 | 0,648 | 0,644 | 0,644 | 0,648 |
inhibition% -3 h |
43 | 26 | 15 | 0 | 1 | 1 | / |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In the test of ISO 8192-1986 (Méthod B) standard, COUMARIN inhibitory concentration of 50% of the oxygen consumption of activated sludge
conditions of urban origin is greater than 463 mg / l for a time incubation of 30 minutes.
For 3 hours of incubation, the EC50 is above 463 mg / l, which is approximately equal to 640 mg / l. - Executive summary:
Introduction: At the request of Christopher Gallet, SC /ORG, FINE/CF &PP(courierCG/DC-92106of12/07/1992) assessingbacterialtoxicityof coumarinwas studied inactivated sludgeusing astandard methodISO 8192, ed.of15/07/1986, entitled "Water Quality-inhibition testoxygen consumptionby activatedsludge"methodB.
Results:
The values corresponds to measured respiratory activity recorded in the laboratory. After 3 hours of incubation sludge still have a significant activity.
The inhibition of respiration of the sludge product was estimated by exceeded 35% after 30 minutes of incubation.
After 3 hours of incubation, the IC50 (inhibitory concentration 50% of the oxygen consumption) was extrapolated by projecting the results on log-probit graph trace of the regression line (see Annex No. l)
Conclusion: In the test of ISO 8192-1986 (Méthod B) standard, COUMARIN inhibitory concentration of 50% of the oxygen consumption of activated sludge conditions of urban origin is greater than 463 mg / l for a time incubation of 30 minutes.
For 3 hours of incubation, the EC50 is above 463 mg / l, which is approximately equal to 640 mg / l.
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