Propanoic acid, 2-hydroxy-, compd. with 7-[[4,6-bis[[3-(diethylamino)propyl]amino]-1,3,5-triazin-2-yl]amino]-3-[2-(2,3-dihydro-2-oxo-1H-benzimidazol-6-yl)diazenyl]-4-hydroxy-2-naphthalenesulfonic acid (1:1)

Short-term toxicity to fish

Description of key information

The 96-hour LC50 is 93 mg/L and the 96-hour NOEC is 22 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

93 mg/L

Additional information

The acute toxicity of the substance to zebra fish (Brachydanio rerio) was determined in a 96-hour static test according to the EU Commission Directive 92/69/EEC, Part C.1 (1992), and the OECD Guideline for Testing of Chemicals No. 203, (1992). The nominal test item concentrations tested were 4.6, 10, 22, 46, and 100 mg/L, and in parallel a control.

The analytically determined test item concentrations in the test media varied in the range from 89 to 99 % of the nominal values. The substance was stable under the conditions of the test during the test period of 96 hours. Therefore, all reported biological results are related to the nominal concentrations of the test item.

The biological test results after 96 hours test duration are:

- 96-hour LC₅₀: 93 mg/L (95% confidence interval not calculable)

- 96-hour LC₀: 46 mg/L

- 96-hour LC₁₀₀: >100 mg/L

- 96-hour NOEC: 22 mg/L

- 96-hour LOEC: 46 mg/L