Dimethyl 5-[[1-[[(2,3-dihydro-2-oxo-1H-benzimidazol-5-yl)amino]carbonyl]-2-oxopropyl]azoterephthalate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1971

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions (e.g. only 7 days post observation period, no details on body weight development)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1971

Report date:

1971

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

(only 7 days of observation p.a.; details on body weight development are missing)

GLP compliance:

no

Remarks:

study was performed previous to GLP implementation

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl 5-[[1-[[(2,3-dihydro-2-oxo-1H-benzimidazol-5-yl)amino]carbonyl]-2-oxopropyl]azoterephthalate

EC Number:

249-955-7

EC Name:

Dimethyl 5-[[1-[[(2,3-dihydro-2-oxo-1H-benzimidazol-5-yl)amino]carbonyl]-2-oxopropyl]azoterephthalate

Cas Number:

29920-31-8

Molecular formula:

C21H19N5O7

IUPAC Name:

dimethyl 5-({2-oxo-1-[(2-oxo-2,3-dihydro-1H-benzimidazol-5-yl)carbamoyl]propyl}diazenyl)isophthalate

Test material form:

solid: nanoform

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: SPF in house breeding colony
- Weight at study initiation: 130 - 170 g
- Fasting period before study: 16 h
- Diet: Standard-ALTROMIN R, Altromin GmbH, Lage, Germany, ad libitum
- Water: tap water, ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: sesame oil

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 25 % suspension in sesame oil

Doses:

15 000 mg/kg bw

No. of animals per sex per dose:

10

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: no

Results and discussion

Effect levels

Key result

Sex:

female

Dose descriptor:

LD50

Effect level:

> 15 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: no animal died within the observation period

Other findings:

Test substance was excreted via the faeces.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Single application of 15000 mg/kg bw of the test substance did not cause lethality in female Wistar rats during the 7 day observation period, resulting in a LD50 > 15000 mg/kg bw.

Executive summary:

Female Wistar rats were subjected to test acute oral toxicity. The test substance was administered by gavage at the highest dose levels applicable: 15000 mg/kg bw. No animal died under these conditions during the 7 day observation period, thus leading to a LD50 > 15000 mg/kg bw.