Benzimidazole-2-thiol

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Experimental data from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

Microtox test: The test exposes luminescent organisms in Microtox Acute Reagent to aqueous samples, and measures the increase or decrease in light output by the test organisms.Reagent contains living luminescent bacteria that have been grown under optimal conditions, harvested,and then lyophilized (freeze-dried). The lyophilized bacteria are rehydrated with Reconstitution Solution to provide a ready-to-use suspension of organisms. The test system measures the light output of the luminescent bacteria after they have been challenged by a sample and compares it to the light output of a control (reagent blank) that contains no sample. A difference in light output (between the sample and the control) is attributed to the effect of the sample on the organisms.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Photobacterium phosphoreum

Test type:

static

Water media type:

freshwater

Total exposure duration:

30 min

Remarks on exposure duration:

5-30 min. exposure period

Hardness:

No data

Test temperature:

15 degC

pH:

range of 5< pH <9

Dissolved oxygen:

No data

Salinity:

No data

Conductivity:

No data

Nominal and measured concentrations:

No data

Details on test conditions:

No data

Reference substance (positive control):

yes

Key result

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

86.4 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other: viability and sensitivity

Validity criteria fulfilled:

not specified

Conclusions:

The median effective concentration (EC50) for the test substance, in microorganism (Photobacterium phosphoreum) in a 5-30 minute study on the basis of viability and sensitivity effect was observed to be 86.4 mg/L.

Executive summary:

The test exposes luminescent organisms in Microtox Acute Reagent to aqueous samples, and measures the increase or decrease in light output by the test organisms.Reagent contains living luminescent bacteria that have been grown under optimal conditions, harvested,and then lyophilized (freeze-dried). The lyophilized bacteria are rehydrated with Reconstitution Solution to provide a ready-to-use suspension of organisms. The test system measures the light output of the luminescent bacteria after they have been challenged by a sample and compares it to the light output of a control (reagent blank) that contains no sample. A difference in light output (between the sample and the control) is attributed to the effect of the sample on the organisms.The median effective concentration (EC50) for the test substance, in microorganism (Photobacterium phosphoreum) in a 5-30 minute study on the basis of viability and sensitivity effect was observed to be 86.4 mg/L.

Description of key information

Toxicity to microorganisms:

The test exposes luminescent organisms in Microtox Acute Reagent to aqueous samples, and measures the increase or decrease in light output by the test organisms.Reagent contains living luminescent bacteria that have been grown under optimal conditions, harvested,and then lyophilized (freeze-dried). The lyophilized bacteria are rehydrated with Reconstitution Solution to provide a ready-to-use suspension of organisms. The test system measures the light output of the luminescent bacteria after they have been challenged by a sample and compares it to the light output of a control (reagent blank) that contains no sample. A difference in light output (between the sample and the control) is attributed to the effect of the sample on the organisms.The median effective concentration (EC50) for the test substance, in microorganism (Photobacterium phosphoreum) in a 5-30 minute study on the basis of viability and sensitivity effect was observed to be 86.4 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:

86.4 mg/L

Additional information

Toxicity to microorganisms:

The test exposes luminescent organisms in Microtox Acute Reagent to aqueous samples, and measures the increase or decrease in light output by the test organisms.Reagent contains living luminescent bacteria that have been grown under optimal conditions, harvested,and then lyophilized (freeze-dried). The lyophilized bacteria are rehydrated with Reconstitution Solution to provide a ready-to-use suspension of organisms. The test system measures the light output of the luminescent bacteria after they have been challenged by a sample and compares it to the light output of a control (reagent blank) that contains no sample. A difference in light output (between the sample and the control) is attributed to the effect of the sample on the organisms.The median effective concentration (EC50) for the test substance, in microorganism (Photobacterium phosphoreum) in a 5-30 minute study on the basis of viability and sensitivity effect was observed to be 86.4 mg/L.