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EC number: 691-657-8 | CAS number: 62599-49-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GPL guideline study without restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-(2-methylprop-2-en-1-yl)cyclododecan-1-one
- EC Number:
- 691-657-8
- Cas Number:
- 62599-49-9
- Molecular formula:
- C16 H28 O
- IUPAC Name:
- 2-(2-methylprop-2-en-1-yl)cyclododecan-1-one
- Details on test material:
- - Name of test material (as cited in study report): 2-(2-Methylallyl)-cyclododecanon-Rein
- Physical state: colorless, clear liquid
- Analytical purity: 99.3 area % (not corrected with the water content)
- Expiration date of the lot/batch: October, 2014
- Stability under test conditions: stable
- Water solubility: 1.4 mg/L at 20 °C.
- Storage condition of test material: at ambient temperature without exposure to light in a tightly sealed container
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations : 1.4, 0.7, 0.35, 0.18, 0.09 mg/L and control (nominal)
- Sampling method: The aim of the analytical part of the definitive test was to determine the concentrations of 2-(2-
Methylallyl)-cyclododecanon-Rein using a validated gas chromatography method with FID detection
[SOP/C/289]. In the definitive test, the samples at exposure initiation and at exposure termination collected from
each test item concentration and the control were analyzed [SOP/W/83].
- Sample storage conditions before analysis: The sample of the test item was stored at ambient temperature without exposure to light in a tightly
sealed container [SOP/W/3, SOP/PB/1].
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
In the definitive test following test item concentrations were used: 1.4, 0.7, 0.35, 0.18 and 0.09 mg/L
plus the control (with a spacing factor of 2.0). The 3 μL of the test item was added into 200 mL of
Elendt M7 medium in glass flask with a capacity of 250 mL using a glass syringe (Hamilton, 10 μL,
Switzerland). The content of flask was thoroughly mixed and, then content of flask was transferred into
glass flask with a capacity of 5 L by multiple washing with Elendt M7 medium. The glass flask was
filled up to 2 L of Elendt M7 medium. The content of flask was ultarsonicated for 5 minutes
[SOP/W78]. After that the test item concentration of 1.4 mg/L was visually transparent and
homogeneous. The test item was visually dissolved. The lower test item concentrations were prepared
by sequential dilutions with the Elendt M7 medium in a ratio of 1 : 1 (volume per volume).
The control (550 mL of Elendt M7 medium) was ultarsonicated for 5 minutes [SOP/W78].
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna Straus
- Source: Institute of Industrial Organic Chemistry, Branch Pszczyna, Department of Ecotoxicology, Laboratory of Aquatic Toxicology.
- Age at study initiation (mean and range, SD): < 24 h old at exposure initiation); not first brood progeny.
- Method of breeding: Daphnia magna was cultured in glass beakers with a capacity of 150 mL (one parent per vessel)
containing 100 mL of Elend M7 medium, at room temperature ranging from 18 to 22°C with daily cycle 16 h light : 8 h dark.
- Feeding during test: no feeding.
ACCLIMATION
- Type and amount of food: suspension of algae; mixture of two species Pseudokirchneriella subcapitata: Desmodesmus subspicatus (in 2:1 ratio) Group B vitamins and micronutrients necessary for proper growth were supplied with the lyophilized suspension of Spirulina sp.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20.0 – 21.0 ºC
- pH:
- 7.52 – 7.53
- Dissolved oxygen:
- 8.9 – 9.6 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 1.4, 0.7, 0.35, 0.18, 0.09 mg/L and control.
Measured concentrations: 1.3, 0.6, 0.32, 1.17, 0.08 and control. - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers of 150 mL (with transparent lids)
- Type : closed
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
The Elendt M7 medium recommended by the OECD Guideline No. 202 (2004) was used to culture the test organism and as a diluent/solvent of the test item.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: daily cycle 16 h light : 8 h dark; fluorescent light source
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Daphnia magna was observed for immobilization after 24 and 48 h of exposure. The Daphnia magna
was considered immobile if they showed no ability to swim within 15 seconds after swirling the test
vessel.
- The temperature was continuously recorded.
- The pH values and the dissolved oxygen concentrations were measured at exposure initiation, i.e.
before the split up into replicates, and at exposure termination in pooled replicates
TEST CONCENTRATIONS
- Range finding study: preliminary test
- Results used to determine the conditions for the definitive study: were determined on the basis of the preliminary tests results - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.48 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence limits: 0.41 – 0.56 mg/L).
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.35 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- - EC50: 0.60 mg/L
- Reported statistics and error estimates:
- - Statistical analysis: Probit method calculations and analysis by Fisher’s Exact Binomial Test with Bonferroni Correction.
Any other information on results incl. tables
S ince the analytically determined concentrations of the test substance in the test solutions were within +20% of the nominal concentrations, the effect concentration can be expressed relative to the nominal concentration. |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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