4-anilino-3-nitro-N-phenylbenzenesulphonamide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

Comparable to international accepted guidelines.

Principles of method if other than guideline:

Method A-11

GLP compliance:

not specified

Specific details on test material used for the study:

None

Analytical monitoring:

not specified

Details on sampling:

Not available

Vehicle:

not specified

Details on test solutions:

No data

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

No data

Hardness:

No data

Test temperature:

23 °C

pH:

8.3-8.4

Dissolved oxygen:

92-96 %

Salinity:

No data

Nominal and measured concentrations:

Nominal: 100, 300 and 1000 mg/L

Details on test conditions:

No data

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

ca. 162 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 540 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 540 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Details on results:

At 96 hrs: 10/10 fishes survived at 100 and 300 mg/L. 9/10 fishes survived at 1000 mg/L.

Results with reference substance (positive control):

No data

Reported statistics and error estimates:

No data

Sublethal observations / clinical signs:

Concentration (mg/L)		Surviving fish					pH	O2 (%)
	1 h	4 h	24 h	48 h	72 h	96 h
100	10	10	10	10	10	10	8.4	96
300	10	10	10	10	10	10	8.4	94
1000	10	10	10	10	10	9	8.3	92

Validity criteria fulfilled:

not specified

Conclusions:

Under the test conditions, the 96 h LC0 for zebra fish (Danio rerio) was 300 mg/L (i.e. ca. 162 mg a.i./L), while the 96 h LC50 and LC100 were both >1000 mg/L (i.e. ca. >540 mg a.i./L).

Executive summary:

A study was conducted according to guideline similar to OECD TG 202 to determine the acute toxicity of the test substance to zebra fish (Danio rerio). Groups of 10 fish were exposed at 100, 300 or 1000 mg/L for a duration of 96 h. The number of surviving fish was recorded at 1, 4, 24, 48, 72 and 96 h. At 96 hrs: 10/10 fishes survived at 100 and 300 mg/L. 9/10 fishes survived at 1000 mg/L. Under the test conditions, the 96 h LC0 was 300 mg/L (i.e. ca. 162 mg a.i./L), while the 96 h LC50 and LC100 were both >1000 mg/L (i.e. ca. > 540 mg a.i./L).

Description of key information

A study was conducted to determine the acute toxicity of the test substance to zebra fish (Danio rerio). Groups of 10 fish were exposed at 100, 300 or 1000 mg/L for a duration of 96 h. The number of surviving fish was recorded at 1, 4, 24, 48, 72 and 96 h. There was no mortality at 100 or 300 mg/L. One fish died within 96 h at 1000 mg/L. Under the test conditions, the 96 h LC0 was 300 mg/L (i.e. ca. 162 mg a.i./L), while the 96 h LC50 and LC100 were both >1000 mg/L (i.e. ca. >540 mg a.i./L). A few more studies performed during 1983, 1975, 1973 have reported TL50 (96 h) >1000 mg/l , >45 mg/L, and 140 mg/L. A 1990 study reported LC50 (96 h) to Zebra fish as >1000 mg/l. In conclusion, the test item FAT 36014 had no acute toxic effects on zebra fish under the present test conditions.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L