Manganese dichloride

Repeated dose toxicity: other routes

Administrative data

Endpoint:

sub-chronic toxicity: other route

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Not to GLP, not to guideline. No data tables, limited information on the test animals, no information on body weight and clinical signs. Follows basic scientific principles.

Data source

Reference

Reference Type:

publication

Title:

Experimental investigation of manganese chloride toxicity

Author:

Pashinskii VG, Tuzlukov AP, Rasskhin VM, Arefe'eva AK, Sedova KS, Motovilova VG and Fil'sanova GA

Year:

1975

Bibliographic source:

Russian Pharmacology and Toxicology , 38(5): 221-224

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Rabbits were given daily doses of 0.5 or 5 mg/kg MnCl2 (i.p) for 2 months. Animals were either sacrificed immediately or had a recovery period of two weeks before extermination.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Manganese dichloride

EC Number:

231-869-6

EC Name:

Manganese dichloride

Cas Number:

7773-01-5

Molecular formula:

Cl2Mn

IUPAC Name:

manganese(2+) dichloride

Test animals

Species:

rabbit

Strain:

not specified

Sex:

not specified

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

water

Duration of treatment / exposure:

2 months

Frequency of treatment:

daily

Doses / concentrations

Remarks:

Doses / Concentrations:
0.5 mg/kg, 5 mg/kg

No. of animals per sex per dose:

No data

Control animals:

yes

Examinations

Observations and examinations performed and frequency:

Haematological parameters assessed along with assessment of the skin and muscles at the injection site. Body organs and tissues also examined.

Results and discussion

Results of examinations

Clinical signs:

no effects observed

Mortality:

no mortality observed

Body weight and weight changes:

not examined

Food consumption and compound intake (if feeding study):

not examined

Food efficiency:

not examined

Water consumption and compound intake (if drinking water study):

not examined

Ophthalmological findings:

not examined

Haematological findings:

no effects observed

Clinical biochemistry findings:

not examined

Urinalysis findings:

not examined

Behaviour (functional findings):

not examined

Organ weight findings including organ / body weight ratios:

no effects observed

Gross pathological findings:

no effects observed

Histopathological findings: non-neoplastic:

not examined

Histopathological findings: neoplastic:

not examined

Details on results:

Levels of Mn in the blood were increased in the rabbits treated with the higher dose of Mn. No changes in the animals behaviour or condition was observed. Levels of Mn in the organs and tissues were within normal parameters. No pathological forms of leukocyte or erythrocyte were observed. Morphologically the haematopoetic organs showed no difference from normal state. Skin on animals at injection site showed no difference from control animals. Muscles at the site of injection (0.5 mg/kg) showed foci of swelling, necrosis and scar tissue. At 5 mg/kg effects were more pronounced. In rabbits with a recovery period of two weeks following the last injection tissues/muscles were fully regenerated, with no visible signs of any pathological changes.

Target system / organ toxicity

Critical effects observed:

not specified

Applicant's summary and conclusion

Conclusions:

Experimental results indicate MnCL2 has a comparatively low toxicity.