2-{4-[4-[4-fluoro-6-(2-(2-vinylsulfonylethoxy)ethylamino)-1,3,5-triazin-2-ylamino]phenylazo]phenylazo}naphthalene-4,6,8-trisulfonate, trisodium salt

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 March 2001 to 08 May 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Identity: FAT 40800/A
Batch number: WP 2/01
Active ingredient: ca. 70 %
Aggregate state at room temperature: Solid / powder
Color: Dark orange
Storage conditions: At room temperature at about 20 °C, away form direct sunlight
Expiration Date: February 14, 2007

Analytical monitoring:

yes

Details on sampling:

Specimens of each test concentration were drawn from the approximate centre of the test vessels. They were taken immediately before the exposure and after 96 hours exposure. If any of the concentrations showed 100 % mortality before the end of the test, specimens were taken when all fish had died.

Details on test solutions:

STOCK SOLUTIONS AND TEST CONCENTRATIONS:
Prior to initiating the definitive test, a static range-finding test was performed with 100 mg test item/L. Based on the results of this range-finding test, the nominal concentration was selected for the definitive test. The stock solution was prepared the following way: 2 g of the test item was mixed with about 200 mL test water in a beaker, homogenised by ultrasonic treatment for 5 minutes and then filled up to 2 L with test water. Calculated amounts of the stock solutions to produce the desired test concentrations were given into the aquaria and filled up to 15 L with dilution water.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Rainbow Trout (Oncorhynchus mykiss), Commercial supplier: P. Hohler/CH- 4314 Zeiningen /Switzerland. Fish supplied in one batch were all of a similar age and size. Fish were held in dechlorinated tap water and fed daily (except for weekends) with a commercially prepared trout food diet: NAFAG No. 8487 S (Nähr- und Fischfutter AG, CH-9202 Gossau). Fish used in the test were not fed 24 hours prior to initiation of the test. Quality of the fish was assessed during an acclimatisation period of at least 12 days in dechlorinated tap water. Fish were not treated for disease during holding.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

142 mg/L CaCO3

Test temperature:

13.4 - 14.0 °C

pH:

7.8 - 8.3

Dissolved oxygen:

95 - 105% of the saturation value

Salinity:

no data

Nominal and measured concentrations:

nominal 100 mg/L, measured 90.56-90.29 mg/L

Details on test conditions:

- Lighting: Fluorescent light, 16 hours daily with 30 min transition period
- Test vessels: 20 litre glass aquaria with a test volume of 15 litre slightly aerated
- Replicates: Performed with one test chamber per concentration and control 7 fish per concentration and control
- Loading: Fish biomass to solution ratio was 0.8 g/L W
- Feeding: Fish were not fed during the test period.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality of fish was recorded in the control and the test concentration of 100 mg/L during the 96 hour exposure period. No sublethal effects were observed in the control or the treatment concentration of 100 mg/L during the 96 hour test period. The mortality in the blank control was 0 %.

Reported statistics and error estimates:

Due to the absence of mortality during the exposure, the median lethal concentration (LC50) was estimated without statistical program.

Validity criteria fulfilled:

yes

Conclusions:

The LC50 (96h) of FAT 40800/A was determined to be greater than 100 mg/L based on nominal concentrations. The highest concentration with no sublethal or lethal effects (NOEC) corresponds to 100 mg/L nominal.

Executive summary:

An acute toxicity test with Rainbow trout (Oncorhynchus mykiss) was performed following the OECD 203 and EU C.1 guideline under GLP. The test was executed as limit test with a concentration of 100 mg/L nominal (and 90.29 mg/L measured). The test was performed as a Static test; test volume of 15 litres; fluorescent light, 16 hours daily. 7 fish at each test concentration were used. No precipitation was observed in the test solution throughout the duration of the test. Measured concentrations of the test item was 90.56 mg/L at the beginning and 90.29 mg/L at the end of the exposure period. Hence, the results are based on the nominal concentrations. No mortality of fish was recorded in the control and the test concentration of 100 mg/L during the 96 hour exposure period. No sublethal effects such as changes in swimming behaviour, respiratory function, pigmentation or loss of equilibrium were observed in the control or the treatment concentration of 100 mg/L during the 96 hour test period. The mortality in the blank control was 0 %. Based on the test results, LC50 (96h) of FAT 40800/A was determined to be >100 mg/L.

Description of key information

The 96-h LC50 of test substance is >100 mg/L in Rainbow trout.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 100 mg/L

Additional information

An acute toxicity test with Rainbow trout (Oncorhynchus mykiss) was performed following the OECD 203 and EU C.1 guideline under GLP. The test was executed as limit test with a concentration of 100 mg/L nominal (and 90.29 mg/L measured). The test was performed as a static test; test volume of 15 litres; fluorescent light, 16 hours daily. 7 fish at each test concentration were used. No precipitation was observed in the test solution throughout the duration of the test. No mortality of fish was recorded in the control and the test concentration of 100 mg/L FAT 40800/A during the 96 hour exposure period. No sublethal effects such as changes in swimming behaviour, respiratory function, pigmentation or loss of equilibrium were observed in the control or the treatment concentration of 100 mg/L during the 96 hour test period. Based on the test results, LC50 (96h) of FAT 40800/A was determined to be >100 mg/L.