Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 606-682-1 | CAS number: 2098-65-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jun - Jul 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 17-Hydroxy-1 alpha,2 alpha-methylene-4,6-pregnadiene-3,20-dione
- EC Number:
- 606-682-1
- Cas Number:
- 2098-65-9
- Molecular formula:
- C22 H28 O3
- IUPAC Name:
- 17-Hydroxy-1 alpha,2 alpha-methylene-4,6-pregnadiene-3,20-dione
- Test material form:
- solid: bulk
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): municipal sewage treatment plant ("Kläranlage Berlin-Ruhleben")
- Preparation of inoculum for exposure: stirred and aerated - Duration of test (contact time):
- 30 d
Initial test substance concentration
- Initial conc.:
- 10 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
The test substance ZK 12126 was incubated in a concentration of 10 mg carbon/L in triplicate. Additionally, a reference substance (sodium acetate) was tested in a single set according to the same pracedure, in order to verify the viability and activity of the degrading micraorganisms. One further set was incubated with sodium acetate as 10 mg carbon/I (reference substance) plus ZK 12126 as 10 mg carbon/L representing a toxicity contra
- Test temperature: 20-24 °C
- pH: 7.8-8.0
- pH adjusted: no
- Sampling days: 3, 4, 7, 9, 11, 14, 18, 23, 28, 30
- Details of trap for CO2 and volatile organics if used: For the determination of the C02 produced, three C02-absorber bottles, filled with 100 mL 0.025 N Ba(OH)2 each, were connected in se ries to the exit air-pipe of each test bottle. The amount of CO2 produced was determined by titration of the remaining Ba(OH)2 with 0.05 N standardised HCI. On days 3, 4, 7, 9, 11, 14, 18, 23, 28 and 30 the CO2-absorber bottle nearest to the test bottles was removed for the titration. The remaining two absorbers were each moved one pi ace closer to the test vessel and a new absorber bottle filled with fresh Ba(OH)2 was placed at the far end of the series.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 3 replicates
- Abiotic sterile control: no
- Toxicity control: yes, 1 replicate
- Reference substance: yes, 1 replicate
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 2
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- The reference compound sodium acetate produced 105 mg CO2, which was equivalent to 96% of the theoretical CO2 production after 28 d
Any other information on results incl. tables
Table 1: Biological degradation (cumulative) in percent (corrected for blank CO2-production) of 1,2-Methylen-4,6-Dien
Test | Nominal | Day of sampling | |||||||||
compound | concentration | 3 | 4 | 7 | 9 | 11 | 14 | 18 | 23 | 28 | 30 |
of carbon | |||||||||||
ZK 12126 | 10 mg/I | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 2 | 2 |
Sodium acetate | 10 mg/I | 14 | 27 | 58 | 67 | 74 | 82 | 88 | 92 | 93 | 96 |
(reference) | |||||||||||
ZK 12126 + | 10 mg/I + | 6 | 13 | 29 | 34 | 37 | 41 | 43 | 45 | 45 | 46 |
sodium acetate (toxicity control) | 10 mg/I |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- In accordance with the OECD guideline, the test compound 1,2 Methylen-4,6-dien is not readily biodegradable under the conditions of the test and it was not toxic to the microbes of activated sludge.
- Executive summary:
The purpose of this study was to determine the ready biodegradability of 1,2 Methylen-4,6-dien. The study was conducted in agreement with the following test guideline: OECD guideline for testing of chemicals, ready biodegradability: CO2-evolution test, no. 301 B, adopted Jul. 92, OECD Paris, 93.
The test substance was incubated in an aqueous solution including nutrients with micraorganisms fram a municipal sewage treatment plant for 28 days (start of treatment = day 1). The nutrient solutions were made up of phosphates, ammonium sulphate, magnesium sulphate, iran chloride, ammonium chloride and calcium chloride. The test substance was incubated in a concentration of 10 mg carbon/L in triplicate. Additionally, a reference substance (sodium acetate) was tested in a single set according to the same pracedure, in order to verify the viability and activity of the degrading micraorganisms. One further set was incubated with sodium acetate as 10 mg carbon/L (reference substance) plus test substance as 10 mg carbon/L representing a toxicity control. Furthermore, a blank control was tested in triplicate without any test or reference substance. The biological degradation of the test and reference substances was evaluated by measurement of the carbon dioxide (CO2) produced during the test period.
The test compound was practically not degraded on day 30 (28 days of incubation). The reference compound sodium acetate was degraded to 96% on day 30. The time required for 60% biodegradation of the reference compound was less than 10 days. In the toxicity control, the reference compound (sodium acetate) plus the test compound was degraded up to 46% on day 30, thus reflecting the degradation of the substances incubated separately.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.