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EC number: 248-387-7 | CAS number: 27287-73-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 February 2016 to 15 March 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Ruhrverband Kläranlage, Sunthelle 6, 57392 Schmallenberg, Germany
- Storage conditions: samples were kept aerobic until use
- Storage length: samples were taken the day of test start
- Preparation of inoculum for exposure: The sludge was left for settlement for ca. one hour. Subsequently the supernatant was discarded and the concentration of suspended solids was measured in the remaining sludge.
- Concentration of sludge: 4.1 g/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 80 mg/L
- Based on:
- test mat.
- Initial conc.:
- 203.6 mg/L
- Based on:
- ThOD
- Remarks:
- NO3 (with nitrification)
- Initial conc.:
- 52.8 mg/L
- Based on:
- ThOD
- Remarks:
- NH3 (without nitrification)
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral medium (please refer to "Any other information on materials and methods incl. tables")
- Test temperature: 22 °C, maintained by a built in thermostat
- pH: 7.2 at the start of test, 7.5 - 8.3 at the end of the test
- pH adjusted: No
- Aeration of dilution water: Yes (continuous stirring)
- Suspended solids concentration: 29.6 mg/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 500 mL reaction vessels
- Number of culture flasks/concentration: two replicates each for test item, inolculum control, procedure control, toxicity control and abiotic control.
- Measuring equipment: Sapromat respirometer (Voith Inc.)
- Details of trap for CO2 and volatile organics if used: soda lime to absorb CO2
SAMPLING
- Sampling frequency: Oxygen consumption was measured and recored contiinuously throughout the duration of the test.
- Sampling method: respirometer
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Abiotic sterile control: Yes
- Toxicity control: Yes - Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- Not performed
- Test performance:
- With a maximum of 9%, the difference of extremes of replicate values of the removal of the test item at the end of the test was less than 20%.
The percentage degradation of the reference item has exceeded the pass level of 60% by day 14. Biodegradation in the toxicity control was >25% by day 14.
The oxygen uptake of the inoculum blank is <60 mg/L in 28 days and the pH value was inside the range of 6.0 - 8.5. - Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- No biodegradation of the test item in the test media could be calculated since less oxygen was consumed in the test media than in the blank inoculum and a standard deviation of 6.4 % based on ThODNH3 and 1.7% based on ThOD NO3 were respectively observed after 28 days of incubation. No biodegradation within the 10-day-window could be calculated since the start criterion of the window (10 % degradation rate) was not reached within the 28 days of incubation.
- Results with reference substance:
- The percent biodegradation of the reference item sodium benzoate was calculated based on the theoretical oxygen demand of 1.67 mg O2/mg (ThOD) In the procedural controls, the reference item was degraded by an average of 87 % by Exposure Day 14, thus confirming suitability of the activated sludge. At the end of the test (Day 28), the reference item was degraded by an average of 94 %.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The ready biodegradability of the test substance was investigated at a concentration of 80 mg/L in a valid manometric respirometry test according to the OECD guideline 301F, using domestic aerobic activated sewage sludge incubated at 22°C in the dark over 28 days under continuous stirring. The test item was not readily biodegradable under the conditions of the test.
- Executive summary:
The ready biodegradability of the test substance was investigated at a concentration of 80 mg/L in a valid manometric respirometry test according to the OECD guideline 301F, using domestic aerobic activated sewage sludge incubated at 22°C in the dark over 28 days under continuous stirring. The biochemical oxygen demand (BOD) was measured daily and the percentage degradation was calculated from the theoretical oxygen demand (ThOD) after correcting for the BOD of inoculum controls.As a procedure control, the reference item sodium benzoate was tested. The toxicity control contained both test material and the reference item sodium benzoate. No biodegradation of the test item in the test media could be calculated since less oxygen was consumed in the test media than in the blank inoculum and a standard deviation of 6.4 % based on ThOD NH3 and 1.7% based on ThOD NO3 were respectively observed after 28 days of incubation.
The test item was found to be not readily biodegradable under the conditions of the test within 28 days.
Reference
The percentage biodegradation of test material and of the reference item sodium benzoate was calculated based on their biochemical oxygen demand (BOD) and theoretical oxygen demand (ThOD). Since the test item contains nitrogen, the percent biodegradation was calculated based on the ThODNH3 (considering that nitrification is absent) and ThODNO3 (considering that nitrification is complete). However, since the biodegradation rate – even considering the total lack of nitrification – was clearly below the threshold value for ready biodegradability, the effects of nitrification did not need to be considered.
The biodegradation of the test item and the reference item in the test over 28 days are presented in the table below:
Time (days) |
Percentage Biodegradation1 |
|||||||||
Test item based on |
Procedure control based on |
Toxicity control based on |
||||||||
ThODNH3 |
ThODNO3 |
ThOD |
ThODNH3 |
ThODNO3 |
||||||
Flask No. |
Flask No. |
Flask No. |
Flask No. |
Flask No. |
||||||
1 |
2 |
1 |
2 |
1 |
2 |
1 |
2 |
1 |
2 |
|
0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
1 |
-8.5 |
-0.9 |
-2.2 |
-0.2 |
30.3 |
29.1 |
7.5 |
2.1 |
4.5 |
1.2 |
2 |
-13.6 |
-1.5 |
-3.5 |
-0.4 |
44.7 |
45.9 |
34.9 |
36.2 |
20.7 |
21.5 |
3 |
-16.7 |
-1.5 |
-4.3 |
-0.4 |
55.5 |
55.5 |
42.6 |
42.6 |
25.3 |
25.3 |
4 |
-22.7 |
-7.6 |
-5.9 |
-2.0 |
63.9 |
64.5 |
49.9 |
52.7 |
29.6 |
31.2 |
5 |
-22.7 |
-7.6 |
-5.9 |
-2.0 |
70.0 |
76.6 |
54.0 |
56.3 |
32.0 |
33.4 |
6 |
-19.7 |
-7.6 |
-5.1 |
-2.0 |
73.6 |
80.2 |
56.8 |
59.0 |
33.6 |
35.0 |
7 |
-16.7 |
-4.5 |
-4.3 |
-1.2 |
77.2 |
82.6 |
59.0 |
60.0 |
35.0 |
35.5 |
8 |
-18.2 |
-6.1 |
-4.7 |
-1.6 |
79.3 |
84.1 |
59.7 |
61.1 |
35.4 |
36.2 |
9 |
-18.2 |
-9.1 |
-4.7 |
-2.4 |
79.9 |
85.3 |
61.1 |
62.0 |
36.2 |
36.7 |
10 |
-18.2 |
-6.1 |
-4.7 |
-1.6 |
81.1 |
86.5 |
61.6 |
62.9 |
36.5 |
37.3 |
11 |
-18.2 |
-6.1 |
-4.7 |
-1.6 |
81.1 |
86.5 |
62.5 |
63.4 |
37.0 |
37.6 |
12 |
-18.2 |
-6.1 |
-4.7 |
-1.6 |
81.1 |
86.5 |
62.9 |
63.8 |
37.3 |
37.8 |
13 |
-22.7 |
-13.6 |
-5.9 |
-3.5 |
85.0 |
88.0 |
62.2 |
63.6 |
36.9 |
37.7 |
14 |
-22.7 |
-10.6 |
-5.9 |
-2.8 |
85.6 |
88.6 |
62.7 |
64.1 |
37.1 |
38.0 |
15 |
-19.7 |
-10.6 |
-5.1 |
-2.8 |
86.2 |
89.2 |
63.1 |
64.5 |
37.4 |
38.2 |
16 |
-19.7 |
-10.6 |
-5.1 |
-2.8 |
86.8 |
90.4 |
63.6 |
65.0 |
37.7 |
38.5 |
17 |
-19.7 |
-10.6 |
-5.1 |
-2.8 |
87.4 |
91.0 |
63.6 |
65.0 |
37.7 |
38.5 |
18 |
-21.2 |
-12.1 |
-5.5 |
-3.1 |
87.7 |
90.7 |
63.8 |
65.2 |
37.8 |
38.6 |
19 |
-18.2 |
-9.1 |
-4.7 |
-2.4 |
88.3 |
91.3 |
64.3 |
66.1 |
38.1 |
39.2 |
20 |
-18.2 |
-9.1 |
-4.7 |
-2.4 |
88.9 |
91.9 |
64.7 |
66.1 |
38.4 |
39.2 |
21 |
-18.2 |
-9.1 |
-4.7 |
-2.4 |
89.5 |
92.5 |
64.7 |
66.6 |
38.4 |
39.4 |
22 |
-18.2 |
-9.1 |
-4.7 |
-2.4 |
90.7 |
92.5 |
64.7 |
67.0 |
38.4 |
39.7 |
23 |
-19.7 |
-10.6 |
-5.1 |
-2.8 |
91.0 |
92.8 |
65.4 |
66.8 |
38.8 |
39.6 |
24 |
-19.7 |
-10.6 |
-5.1 |
-2.8 |
91.6 |
93.4 |
65.9 |
66.8 |
39.0 |
39.6 |
25 |
-16.7 |
-7.6 |
-4.3 |
-2.0 |
92.2 |
94.0 |
66.3 |
67.7 |
39.3 |
40.1 |
26 |
-16.7 |
-7.6 |
-4.3 |
-2.0 |
92.8 |
94.6 |
66.3 |
67.7 |
39.3 |
40.1 |
27 |
-18.2 |
-9.1 |
-4.7 |
-2.4 |
93.1 |
94.9 |
66.6 |
67.9 |
39.4 |
40.3 |
28 |
-18.2 |
-9.1 |
-4.7 |
-2.4 |
93.1 |
94.9 |
67.0 |
68.4 |
39.7 |
40.5 |
Mean (Day 28) |
-13.6 |
-3.5 |
94.0 |
64.7 |
40.1 |
1 Corrected for the mean oxygen uptake of the inoculum
Description of key information
The test substance was not readily biodegradable in a valid manometric respirometry test according to OECD 301F.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
No biodegradation of the test item in the test media could be calculated since less oxygen was consumed in the test media than in the blank inoculum and a standard deviation of 6.4% based on ThODNH3 and 1.7% based on ThODNO3 were respectively observed after 28 days of incubation. No biodegradation within the 10-day window could be calculated since the start criterion of the window (10% degradation rate) was not reached within the 28 days of incubation.
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