Carbonic acid, 4-nitrophenyl 5-thiazolylmethyl ester, hydrochloride (1:1)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13 Feb - 17 Feb, 1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: done under GLP and OECD method

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

Method C1, Directive 92/69/EEC

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

414-490-2

EC Name:

-

Cas Number:

154212-59-6

Molecular formula:

C11H9ClN2O5S

IUPAC Name:

4-nitrophenyl (1,3-thiazol-5-yl)methyl carbonate hydrochloride

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

Test substance dissolved in 1% Tween 80 dimethylformamide

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Rainbow Trout (Oncorhynchus mykiss),
Mean std length of fish: 6.8cm (SD: 0.7cm)
Mean wt of fish: 4.2g (SD: 1.68g)

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

154 ± 12mg CaCO3/L

Test temperature:

14°C ± 1°C (mean temp: 15°C)

pH:

7.6 - 7.8 (mean values)

Dissolved oxygen:

9.8 ± 0.4mgO2/L

Nominal and measured concentrations:

Nominal concs of test solution: 0.43, 0.93, 2.0, 4.3 and 9.3mg/l
Measured concs of test solns: 0.12, 0.28, 0.80, 1.9 and 6.0mg/l

Details on test conditions:

Photoperiod: 16hrs light, 8hrs dark.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 0.9 -1.7 CL 95%

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1.2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Highest conc. resulting in 0% mortality: 0.80mg/l, measured
Lowest test conc. resulting in 100% mortality: 1.9mg/l, measured.
NOEL.: 0.28mg/l, measured.

Results obtained at 3, 6, 24, 48, 72 and 96hrs.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 for the test substance is estimated to be 1.2mg/l (96hr)