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EC number: 922-676-4 | CAS number: 286426-31-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.5265 (The Salmonella typhimurium Bacterial Reverse Mutation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3-[(1S)-2-[benzyl(methyl)amino]-1-hydroxyethyl]phenol
- Cas Number:
- 286426-31-1
- Molecular formula:
- C17 H20 O2
- IUPAC Name:
- 3-[(1S)-2-[benzyl(methyl)amino]-1-hydroxyethyl]phenol
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Physical state: solid
- Analytical purity: 99.7 %
- Storage condition of test material: room temperature
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Test concentrations with justification for top dose:
- Without metabolic activation : TA 1535 10 µg/plate
TA 100 10 µg/plate
TA 98 10 µg/plate
TA 1537 40µg/plate
TA 102 1µl/plate
With metabolic activation : for all strains except TA 102 2.5µg/plate
TA 102 10µg/plate
- Vehicle / solvent:
- Without metabolic activation: aqua dest or DMSO
With metabolic activation : DMSO
Solvent controls, consisting of the extraction vehicle alone and treadted in the same way as the treatment groups were included.
In additiona, untreated controls were used unless there are historical control data demonstrating that no deleterious or mutagenic
effects are induced by the chosen solvent.
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar
- Evaluation criteria:
- A test item is considered as mutagenic if : - a dose related increase in the number of revertants occurs and / or
- a reproductible biologically relevant positive response for at least one of the test points occurs
in at least one strain with or without metabolic activation.
A biologically relevant increase is described as follows : - if in strains TA 100 and TA102 the number of reversions is at least twice as high
- if in strains TA 1535,TA 1537 and TA 98 the number of reversions is at least three times higher
as comparedto the spontaneous reversion rate.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
In conclusion, it can be stated that during the decribed mutagenicity test and under the experimental conditions reported, the test item did not cause
gene mutations by base pair changes or frameshifts in the genome of the test strains used. Therefore, L_benzyladrianol Base is considered to be
non-mutagenic in this bacterial reverse mutation assay. - Executive summary:
In order to investigate the pontential of L-Benzyladrianol Base for its ability to induce gene mutations the plate incorporation test
( experiment I ) and the percubation test ( experiment II ) were performed with the Salmonella typhiurium strains TA 1535, TA 1537,
TA 98, TA 100 and TA 102.
The test item was tested in two independent experiments at serveral concentrations. Each assay was conducted with and without activation ( S9 mix ).The concentration, including the controls, were tested in triplicate. The following concentrations of the test item
were prepared and used in the experiment : 31.6,100.0,316.2,1000.0,2500.0 and 5000.0 µg/plate.
due to toxicity: exp. II ; TA 1535,TA 102 and TA 100 : 10.0,31.6,100.0,316.2,1000.0 and 2500.0 µg/plate.
Toxic effects of the test item ( indicated by reduction of the background lawn or by distinct reduction of the spontaneous rate ) were
observed in both experiments at the higher investigated doses in all strains used.
No substantial increase in the revertant colony number of any of the five test starins were detected at any dose level of the test item either with or without metabolic actiavtion in both independent performed experiments.
As positive controls reference mutagens were tested in paralell to the test item. They showed distinct of induced revertant colonies.
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