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Diss Factsheets
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EC number: 938-980-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 10 - 12, 2013
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Qualifier:
- according to guideline
- Guideline:
- other: UNI EN ISO 6341:2004 “Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea)”.
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Series on Testing and Assessment No 23 - Guidance Document On Aquatic Toxicity Testing Of Difficult Substances And Mixtures ENV/JM/MONO(2000)6.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Glycine max. (L.) Merr. (Fabacee) aqueous alcoholic ext., concentrate by sequential extractions and filtration
- EC Number:
- 938-980-5
- IUPAC Name:
- Glycine max. (L.) Merr. (Fabacee) aqueous alcoholic ext., concentrate by sequential extractions and filtration
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- Test item identification : Soybean (Glycine max) dry purified extract
Batch number : 30432/M1
Internal number : 2912125-001
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The study was performed on the freshwater crustacean Daphnia magna Straus. A strain of this species was supplied by MicroBioTests Inc., Belgium.
the clone has been bred in the laboratories of the Test Facility under conditions identical to those of the test, with regard to temperature, light and water quality (see below), so that no acclimation was needed prior to testing.
It was daily fed with a fixed amount of the laboratory cultured green algae Pseudokirchneriella subcapitata (formerly Selenastrum capricornutum) and with a suspension of the yeast Saccharomyces cerevisiae.
At the test start the organisms used in the test were less than 24 hours old and were not first brood progeny.
For the test concentration and for the negative control, 20 daphnids were used divided into four replicates of five daphnids each.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- The daphnids were observed for immobility after 24 and 48 hours of exposure.
Test conditions
- Hardness:
- 140 - 250 mg/L as CaCO3
- Test temperature:
- the incubation temperature was continuously monitored during the course of the study by a PT100 probe installed in the environmental test chamber. It was in the range 19.9 – 20.1 °C with a mean value of 20.0 °C and a standard deviation of 0.02 °C. The temperature range provided by OECD guideline is 20 ± 2 C. The temperature values during the test were within the provided range.
- pH:
- The pH of the test media after preparation was 6.86 for test item solution and 7.55 for negative control. The pH did not vary by more than 1.12 unit during the test period.
- Dissolved oxygen:
- The dissolved oxygen concentrations in the test media and the negative control were always higher than6.0 mg/L according to OECD Guideline N 202 that provides a minimum oxygen concentration of 3.0 mg/L.
- Nominal and measured concentrations:
- 70.0 mg/L was founded to be the maximum soluble concentration in reconstituted water.
- Details on test conditions:
- The test medium was prepared as follows:
prior to the test start, 0.5 litre of a 70.0 mg test item /L stock solution was prepared by direct weighting into reconstituted water (0.0350 g of test item into 500 mL of reconstituted water). The obtained solution appeared cloudy and showed insoluble particles; therefore, it was sonicated for about 20 minutes and after was mixed by the mean of a magnetic stirrer for about 48 hours in dark conditions. After stirring the solution appeared lightly brown but completely solubilised.
Light:
a 16 hours light and 8 hours darkness daily photoperiod. The measured values were in the range 893 – 920 Lux, according to provided range (700-1000 Lux). - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate solution
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- IC100
- Effect conc.:
- > 70 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 70 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 70 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Linear Interpolation method
- Details on results:
- - Results with reference substance valid? YES
- No. of immobilized daphnia: 0/20
- EC50/LC50: > 70 mg/l - Results with reference substance (positive control):
- - Results with reference substance valid? YES
- No. of immobilized daphnia: 0/20
- EC50/LC50: > 70 mg/l
- Other:
Any other information on results incl. tables
At the end of test period, in the negative control 0 % of immobilization was observed and no daphnid showed signs of disease or stress (e.g. to be trapped on the test water surface). These values comply with the validity criteria of the test, that provide a maximum immobilization or a maximum number of daphnids showing desease or stress signs of 10 % in the negative control medium at the end of the test.
The obtained experimental results allowed to calculate the IC0, IC100 and the IC50 values and the NOEC at 24 and 48 hours. The results, based on the nominal concentrations of test item, were as follows:
Time (h) |
IC 0 (mg/L) |
IC 100 (mg/L) |
IC 50 (mg/L) |
NOEC (mg/L) |
24 |
70.0 |
> 70.0 |
> 70.0 |
70.0 |
48 |
70.0 |
> 70.0 |
> 70.0 |
70.0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The IC50 of the substance based o the nominal concentration is > 70mg/L/48h.
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