Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 613-147-6 | CAS number: 63141-09-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06 Nov - 08 Nov 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- TOC determination
- Details on sampling:
- - Concentrations: 6.3, 1.2, 5, 25, 50 and 100 mg/L
- Sampling schedule:
Control: at 0 and 48 hours
Test concentration: at 0 and 48 hours - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Direct weighings were prepared to give the desired test concentrations. To achieve this
the test substance was added to 1 litre of dilution water for each test concentration and treated, with an ultra tunax for 60 seconds at 3000 rpm.
- Controls: Yes
- Test concentration separation factor: 2
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain: Strain of Bundesgesundheitsamt Berlin
- Stage and instar at study initiation: neonates
- Method of breeding: parthenogenetic females
- Source: A population of parthenogenetic females of synchronized age structure is maintained since more than 15 years in the test facility.
- Age of parental stock: maintained since more than 15 years in the test facility.
- Feeding during test: no feeding during the exposure period - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 15.6 °dH (= 278.5 mg/L CaCO3)
- Test temperature:
- 19.9 - 20 °C
- pH:
- 7.9
- Dissolved oxygen:
- 94 - 99 %
- Nominal and measured concentrations:
- Nominal = 6.3, 12.5, 25, 50 and 100 mg/L
Mean Measured = 2, 7, 13, 38 and 78 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Karlsruher bottles holding 10 neonates in 250-300 mL of test medium
- open or closed: closed bottle conditions
- Volume of solution: 250-300 mL
- Aeration: none
- No. of organisms per vessel: 10 neonates per vessel
- No. of vessels per concentration: 2 replicates per concentration
- No. of vessels per control: 2 replicates per control
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water (so-called 'M4 medium', originally described in Water Research 24 (9), September 1990: 1157-1167), prepared according to the recommendations of Bundesgesundheitsamt Berlin.
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark
EFFECT PARAMETERS MEASURED: Induced alteration of the normal mobility behaviour and the loss of locomotory actions of the neonates, observed at 24 and 48 hours
RANGE-FINDING STUDY
- Test concentrations: not reported
- Results used to determine the conditions for the definitive study: Yes - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 78 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 13 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Other biological observations: none
- Mortality of control: 0 %
- Immobilisation of control: 0 % - Reported statistics and error estimates:
- Probit analysis
- Validity criteria fulfilled:
- yes
- Remarks:
- See more details in the table in the section "Any other information on results incl. tables"
- Conclusions:
- The test substance affected the mobility of daphnia magna with EC0 =13 mg/L and EC100=78 mg/L (mean measured concentration).
- Executive summary:
The 48 h-acute toxicity of the test substance to Daphnia magna was studied under static conditions. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (OJ. No. L383A, 29.12.92) Part C, Method 2 'Acute toxicity for Daphnia'. Test species were exposed to control and test chemical at nominal concentrations of 6.3, 12.5, 25, 50 and 100 mg/L (mean measured concentrations: 2, 7, 13, 38 and 78 mg/L) for 48 h. Immobilisation and locomotion effects were monitored daily. Analysis of the immobilisation rates (by probit analysis) resulted in 48 h EC0 and EC100 of 13 and 78 mg/L, respectively. The results are expressed in terms of mean measured concentrations. Measured concentrations ranged from 32 - 82% of nominal values at 0 hours, and from 32 - 76% of nominal values at 48 hours, respectively.
Reference
Validity criteria for the measurement of the fish toxicity
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
In the control, including the solubilising agent, not more than 10 % of the daphnids should have been immobilized. (Not more than 10 % of the control daphnids should show immobilisation or other signs of disease or stress, for example, discoloration or unusual behaviour such as trapping at surface of water) | No immobilization observed in control | Yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/l in control and test vessels. | Dissolved oxygen concentration was atleast 8.5 mg/L in control and test vessels. | Yes |
The nominal test concentrations and the result of all analyses to determine the concentration of the test substance in the test vessels; the recovery efficiency of the method and the limit of determination should be reported. | Adequate information was reported | Yes |
The pH should not vary by more than 1 unit | The pH recorded was 7.9 | Yes |
There should be evidence that the concentration of the test substance has been satisfactorily maintained, and preferably it should be at least 80% of the nominal concentration throughout the exposure. If the deviation from the nominal concentration is >20%, results should be based on the measured concentration (geometric mean in static and semi-static tests, arithmetic mean in flow-through tests; time weighted average, where applicable). | Measured concentration for all concentrations at 0 and 48 h were < 80 % of nominal concentration except the measured concentration of the highest concentration (100 mg/L) at 0 h. Nevertheless, the LC50 was based on the mean of the measured concentration during the 0 - 48 h exposure period. | Yes |
Description of key information
The 48 h-acute toxicity of the test substance to Daphnia magna was studied under static conditions. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (OJ. No. L383A, 29.12.92) Part C, Method 2 'Acute toxicity for Daphnia'. Test species were exposed to control and test chemical at nominal concentrations of 6.3, 12.5, 25, 50 and 100 mg/L (mean measured concentrations: 2, 7, 13, 38 and 78 mg/L) for 48 h. Immobilisation and locomotion effects were monitored daily. Analysis of the immobilisation rates (by probit analysis) resulted in 48 h EC0 and EC100 of 13 and 78 mg/L, respectively. The results are expressed in terms of mean measured concentrations. Measured concentrations ranged from 32 - 82% of nominal values at 0 hours, and from 32 - 76% of nominal values at 48 hours, respectively.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 13 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.