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EC number: 203-581-0 | CAS number: 108-42-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Long-term toxicity to fish
Administrative data
- Endpoint:
- fish early-life stage toxicity
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Relaible source.
Data source
Reference
- Reference Type:
- publication
- Title:
- Quantitative structure-activity relationships for fish early life stage toxicity
- Author:
- van Leeuwen, C. J. et al.
- Year:
- 1 990
- Bibliographic source:
- Aqualic Toxicology, 16 ( 1990) 321-334
Materials and methods
- GLP compliance:
- no
Test material
- Reference substance name:
- 3-chloroaniline
- EC Number:
- 203-581-0
- EC Name:
- 3-chloroaniline
- Cas Number:
- 108-42-9
- Molecular formula:
- C6H6ClN
- IUPAC Name:
- 3-chloroaniline
- Test material form:
- other: liquid
- Details on test material:
- - Purity: 98 (Supplier: Merck-Schuchardt)
- Empirical formula: C6H6ClN
- Molecular mass: 127.58 g/mole
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The ratio between the concentrations was 1.8. The test solutions were renewed 3 times a week. In the tests with 3-chloroaniline, the test solutions were gently aerated. In several instances dimethyl sulphoxide (DMSO) was used as solvent for 3-chloroaniline. DMSO concentrations were kept below 100 µL/L. The effects of DMSO were verified in solvent control experiments. pH and oxygen concentrations were measured at regular intervals.
The photoperiod was 12 hours.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Fertilized eggs of zebra fish (Brachydanio rerio) in the blastula stage were obtained from a stock culture at the TNO Iabaratory of 50-100 eggs ( < 6 h
after spawning) into 1-liter glass test vessels filled with 1-liter test solution. After one day all non-viable eggs were remcived and the number of viable eggs was reduced to a maximum of 40 per concentration. In case the number of viable eggs in the controls fell below 25 after 48 h, the test was discarded. The embryolarval stages were exposed in a semistatic manner to 7-8 toxicant concentrations and a control for a period of 28 days. Upon completion of hatching (4-5 days), the fry were transferred into two vessels per concentration. The fry were fed equal amounts of the rotifer Brachionus rubens, obtained from a Iabaratory culture. After 7 days this food was supplemented by 48-h old nauplii of the brine shrimp Artemia salina. The nauplii were enriched with Selco, a commercial concentrate for nutritional enrichment of live food for fish.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 28 d
Test conditions
- Hardness:
- 210 mg/L CaCO3 = 11.8 °dH
- Test temperature:
- 24 ± 2°C
- pH:
- 8.0 -8.2
- Dissolved oxygen:
- 7.7 mg/L
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 28 d
- Dose descriptor:
- LC50
- Effect conc.:
- 6.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: (5.8 - 8.0 mg/L)
- Duration:
- 28 d
- Dose descriptor:
- other: NOCL
- Effect conc.:
- 5.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
Any other information on results incl. tables
Calculations and statistics
The LC50 and 95% confidence Iimits (C.L) were calculated according to Kooyman (1981). If a test yielded concentrations without partial kills, the geometric mean of the 0 and 100% effect concentrations was taken as the LC50 and binomial confidence limits were calculated (Stephan, 1977). In order to calculate the no observed lethal concentration (NOLC: the highest concentration tested without significant effects on survival) and no observed effect concentration (NOEC: the highest concentration tested without; significant effects on survival, hatching and growth), a two-stage approach was applied to exclude any possible effects of size-selective mortality. First the NOLC was determined. Differences in mean survival in the experimental concentrations were tested against the blank control by means of a x2 test (Sokal and Rohlf, 1981). Differences in mean length between treatments and blank control were tested using procedures described by Williams (1971, 1972), after verifying the differences between blank and solvent controls. The Williams' test was applied only to those concentrations which were equal to or below the NOLC. Differences were considered to be significant at α = 0.05.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The early life stage toxicity to fish (Danio erio) was tested in a semistatic test. After 28 days of exposure a LC50 of 6.8 mg/L was measured and a NOLC of 5.6 mg/L and a NOEC of 1.0 mg/L was calculated.
- Executive summary:
The early life stage toxicity to fish (Danio erio) was tested in a semistatic test. After 28 days of exposure a LC50 of 6.8 mg/L was measured and a NOLC of 5.6 mg/L and a NOEC of 1.0 mg/L was calculated.
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