N,N'-bis(1,3-dimethylbutylidene)-2,2'-iminobis(ethylamine)

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

publication

Title:

Robust Summaries for Diethylenetriamine, 1,7-bis(1,3-dimethylbutylidene)

Author:

US EPA

Year:

2003

Bibliographic source:

US EPA, HPVIS, 201-14737B

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

not specified

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

N,N'-bis(1,3-dimethylbutylidene)-2,2'-iminobis(ethylamine)

EC Number:

234-205-3

EC Name:

N,N'-bis(1,3-dimethylbutylidene)-2,2'-iminobis(ethylamine)

Cas Number:

10595-60-5

Molecular formula:

C16H33N3

IUPAC Name:

N-(1,3-dimethylbutylidene)-N'-{2-[(1,3-dimethylbutylidene)amino]ethyl}ethane-1,2-diamine

Details on test material:

The test substance is produced in the presence of excess Methyl Isobutyl Ketone (MIBK) (~30%). At this concentration, the material is a clear, light yellow, very fluid liquid. An attmept was made to drive off the MIBK by distillation when the test substance was prepared for HPV testing. However, this attempted removal of the excess MIBK solvent from the substance resulted in formation of polymeric by-products. Therefore, the production batch (70% test substance in 30% MIBK) was used for testing.

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

no data

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

no data

Doses:

1.0, 2.0, 4.0 ml/kg

No. of animals per sex per dose:

5

Control animals:

not specified

Details on study design:

Groups of five male and five female fasted Albino rats were
dosed with the undiluted sample at dosage levels of 4, 2, and
1 ml/kg. Animals were observed for signs of toxicity and
mortality. Weight changes were measured in 14 day study
period. Necropsies were performed on all animals upon death
or 14 days after dosing.

Statistics:

no data

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 1 673 mg/kg bw

Based on:

test mat.

Remarks on result:

other: 1.88 ml/kg in the original report, density used for calculation: 0.89g/cm3

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 1 895 mg/kg bw

Based on:

test mat.

Remarks on result:

other: ca. 2.13 ml/kg bw

Effect levels 3

Sex:

female

Dose descriptor:

LD50

Effect level:

ca. 1 459 mg/kg bw

Based on:

test mat.

Remarks on result:

other: ca. 1.64 ml/kg bw

Mortality:

All animals dosed at 4.0 ml/kg died within one day of dosing. Six animals died from one to nine days after dosing in the 2.0 ml/kg group. No animals treated with 1 ml/kg died.

Clinical signs:

no data

Body weight:

no data

Gross pathology:

no data

Other findings:

no data