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EC number: 271-808-0 | CAS number: 68608-89-9 This substance is identified by SDA Substance Name: C11-C13 branched alkyl benzene sulfonic acid sodium salt and SDA Reporting Number: 25-097-04.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- May 16, 1995-June 30, 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts
- EC Number:
- 270-115-0
- EC Name:
- Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts
- Cas Number:
- 68411-30-3
- Molecular formula:
- C16-19 H25-31 Na O3 S
- IUPAC Name:
- sodium 4-undecylbenzenesulfonate
- Reference substance name:
- Benzenesulfonic acid, C10-13 alkyl derivatives, sodium salt (LAS)
- IUPAC Name:
- Benzenesulfonic acid, C10-13 alkyl derivatives, sodium salt (LAS)
Constituent 1
Constituent 2
Method
Species / strain
- Species / strain / cell type:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 from aroclor 1254 induced rat liver
- Test concentrations with justification for top dose:
- 0, 0.6, 1, 1.8, 3, 6 ug/ml without S9
0, 6, 10, 18, 30, 60 ug/ml with S9 - Vehicle / solvent:
- None
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- no
- True negative controls:
- yes
- Remarks:
- H0 medium
- Positive controls:
- yes
- Positive control substance:
- other: ethyl methane sulfonate; 3-(20-)methylcholanthrene
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium
DURATION
- Preincubation period: 1 week
- Exposure duration: 4 hrs
- Expression time (cells in growth medium): 6 days at 37 degree C for cloning efficiency study, 9 days for mutation assay
STAIN (for cytogenetic assays): Giemsa
NUMBER OF REPLICATIONS: 2
- Evaluation criteria:
- A test substance was considered mutagenic if a statistically significant dose-related increase in mutant frequency was found in concentrations with greater than 20% survival rate. The mean mutant frequency must also be significantly above the maximum spontaneous mutant frequency.
- Statistics:
- Statistical significance was determined by the t-test.
Results and discussion
Test results
- Species / strain:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- preliminary test showed cytotoxicity at >= 50 ug/ml without S9, and >= 100 ug/ml with S9.
- Vehicle controls validity:
- not examined
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- COMPARISON WITH HISTORICAL CONTROL DATA: In both the studies with and without S9, the mutant frequencies in the treated groups were statistically significantly higher than in the concurrent negative controls. However, the mutant frequencies in the treated groups were not significantly increased when compared to historical negative controls. There was also no dose-response relationship. The increased mutant frequency in treated groups was therefore not considered to be biologically significant.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Results of Test 1 – Without S9 Mix
Concentration (ug/ml) |
Absolute cloning efficiency (%) |
Mutant frequency ( x 106) |
0 |
82 |
3 ± 2 |
0.6 |
86 |
7 ± 1 |
1 |
85 |
3 ± 2 |
1.8 |
78 |
5 ± 2 |
3 |
86 |
1 ± 1 |
6 |
83 |
0 ± 1 |
EMS |
83 |
277 ± 17 |
Results of Test 1 – With S9 Mix
Concentration (ug/ml) |
Absolute cloning efficiency (%) |
Mutant frequency ( x 106) |
0 |
90 |
2 ± 1 |
6 |
88 |
1 ± 1 |
10 |
84 |
9 ± 4 |
18 |
78 |
5 ± 3 |
30 |
89 |
3 ± 2 |
60 |
89 |
7 ± 2 |
MCA |
81 |
91 ± 9 |
Results of Test 2 – Without S9 Mix
Concentration (ug/ml) |
Absolute cloning efficiency (%) |
Mutant frequency ( x 106) |
0 |
96 |
1 ± 1 |
0.6 |
92 |
2 ± 3 |
1 |
95 |
1 ± 1 |
1.8 |
93 |
5 ± 2 |
3 |
90 |
2 ± 1 |
6 |
91 |
6 ± 6 |
EMS |
90 |
309 ± 20 |
Results of Test 2 – With S9 Mix
Concentration (ug/ml) |
Absolute cloning efficiency (%) |
Mutant frequency ( x 106) |
0 |
90 |
2 ± 1 |
6 |
92 |
7 ± 3 |
10 |
88 |
9 ± 2 |
18 |
94 |
2 ± 1 |
30 |
93 |
2 ± 2 |
60 |
90 |
5 ± 1 |
MCA |
95 |
89 ± 6 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The test substance is not mutagenic in either the presence or absence of metabolic activation. - Executive summary:
This study examined the potential of the test substance (LAS) to cause mutations in mammalian cells. Chinese Hamster Ovary (CHO) cells were exposed to concentrations of 0, 0.6, 1, 1.8, 3, and 6 ug/ml without S9, and 0, 6, 10, 18, 30, and 60 ug/ml with S9. The cells were then examined for cytogenicity and mutation frequency. Ethyl methane sulfonate and 3-(20-)methylcholanthrene were used as positive control substances. Preliminary tests show the test substance was cytogenic at concentrations of 50 ug/ml or greater with metabolic activation, and 100 ug/ml or above without metabolic activation. There was no biologically significant increase in mutation frequency in the treated groups. The test substance is considered not mutagenic to CHO cells both in the presence and absence of S9.
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