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Diss Factsheets
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EC number: 204-393-1 | CAS number: 120-40-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- November 7 to November 17, 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- KL 2 due to RA and no analytical dose verification.
- Justification for type of information:
- Refer to the section 13 for details on the category justification.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- no analytical verification of test conc was done
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test solution was prepared in dilution water. The test material was found to be soluble up to 1000 mg/L in water.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain: Not specified
- Source: Toxicon's Daphnia culture
- Age at study initiation: Newly hatched, 6 to 24 h old
- Weight at study initiation: Not specified
- Length at study initiation: Not specified - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
- Hardness:
- Not specified
- Test temperature:
- 21+/-1°C
- pH:
- 7.8+/-0.2
- Dissolved oxygen:
- Not specified, initial oxygen saturation > 90%
- Salinity:
- 250+/-25 mg CaCO3/L
- Nominal and measured concentrations:
- Nominal: 0, 1, 2, 4, 8 and 16 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: Glass petri dishes with 50 mL solution
- Aeration: The dilution water was initially aerated to an oxygen saturation value of > 90%
- Renewal rate of test solution: Not applicable, static test
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): Two
- No. of vessels per control (replicates): Two
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reverse osmosis water
Composition of dilution water: According to ISO test water composition
OTHER TEST CONDITIONS
- Adjustment of pH: When required, it was done with HCl or NaOH
- Photoperiod: 16 h/8 h light: dark
- Light intensity: Subdued, no oher details
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobilisation in the control and test animals at 24 and 48 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- not required
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Control dish: Less than 10% immobility was observed in the control at 24 and 48 h.
Test dish: At 16 and 8 mg/L, 100 % immobilisation was observed.
Oxygen saturation: > 97% oxygen saturation was maintained at the end 48 h. The pH was maintained around 8.0.
For details please refer to the attached results table in the 'background material' section. - Validity criteria fulfilled:
- yes
- Remarks:
- Both the criteria for dissolved oxygen and control immobility were fulfilled
- Conclusions:
- Under the study conditions, the nominal 48 h EC50 for the read across substance was determined to be 3.2 mg/L. The nominal 48 h LOEC and NOEC values were 2.0 and 1.0 mg/L, respectively.
- Executive summary:
A study was conducted to determine the acute toxicity of the read across substance, C8-18 and C18-unsatd. DEA, to Daphnia magna according to OECD Guideline 202, in compliance with GLP. The daphnids were exposed to test substance (directly diluted in test medium) at 0, 1, 2, 4, 8 and 16 mg/L for 48 h under static conditions. Observations were performed at 0, 24 and 48 h to determine the % of immobilised daphnids in the control and test vessels. No analytical dose verification was conducted. Under the study conditions, the nominal 48 h EC50 was determined to be 3.2 mg/L. The nominal 48 h LOEC and NOEC values were 2.0 and 1.0 mg/L, respectively (Olsson, 1994).
Reference
None
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 3.2 mg/L
Additional information
A study was conducted to determine the acute toxicity of the read across substance, C8-18 and C18-unsatd. DEA, to Daphnia magna according to OECD Guideline 202, in compliance with GLP. The daphnids were exposed to test substance (directly diluted in test medium) at 0, 1, 2, 4, 8 and 16 mg/L for 48 h under static conditions. Observations were performed at 0, 24 and 48 h to determine the % of immobilised daphnids in the control and test vessels. No analytical dose verification was conducted. Under the study conditions, the nominal 48 h EC50 was determined to be 3.2 mg/L. The nominal 48 h LOEC and NOEC values were 2.0 and 1.0 mg/L, respectively (Olsson, 1994).
A study was conducted to evaluate the acute toxicity of the read across substance, C12-18 and C18-unsatd. DEA, to Daphnia magna according to OECD Guideline 202 with deviations. Daphnia (20 per dose) were exposed to the test substance (directly diluted in test medium) in a concentration range of 1-11 mg/L for 24 h and immobility was determined at test end. No analytical dose verification was carried out. Under the study conditions, the nominal 24 h EC0, EC50 and EC100 were 2.0, 3.3 and 5.6 mg/L, respectively (Gode, 2001).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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