3-(isodecyloxy)propiononitrile

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

yes

Remarks:

Please see below

Principles of method if other than guideline:

Deviations from Guideline:

Use of copper (lI) sulfate pentahydrate as reference substance instead of 3,5-dichlorophenol, which is classified as carcinogenic. Therefore, it can not be used in the laboratory routine operation.

Activated sludge washed twice and not three times. This has proven to be sufficient.

Dry weight of activated sludge provided by guideline: 4 [g / L] ± 10 %. Since the activated sludge of Hildesheim Kläranlage is of comparatively high activity,
a lower sludge dry weight of 3 [g / L] ± 20 % was used. For the measurement of respiration this is proved to be suffici

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3-(isodecyloxy)propiononitrile

EC Number:

264-840-1

EC Name:

3-(isodecyloxy)propiononitrile

Cas Number:

64354-92-3

Molecular formula:

C13H25NO

IUPAC Name:

3-(decylalkyl-(branched)oxy)-propiononitrile

Test solutions

Details on test solutions:

200 [ml] inoculum + 16 [mL] synthetic wastewater + 284 [ml] aqua demin. + test substance was used

dispersion process: 30 [min] ultrasound at 40 [°C]

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

Origin: sewage treatment plant, Hildesheim, Germany, final clarifier

pH: 7.15
dry weight: 2.7 g/L

Freshly sludge from sewage treatment plant Hildesheim was used after washing twice with autoclaved tap water and dilution 1:3.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test conditions

Test temperature:

18-22°C

Dissolved oxygen:

permanent ventilation by aquarium pumps

Nominal and measured concentrations:

100 - 320 - 1000 - 3200 - 10000 [mg/L]

Reference substance (positive control):

yes

Remarks:

copper (lI) sulfate pentahydrate (99-100%)

Results and discussion

Effect concentrations

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

2 514 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: 1855-3408 mg/L

Details on results:

The concentration-response relationship of the test substance was plotted in the probability grid.
The EC50 and confidence limits were determined by probit analysis.

Results with reference substance (positive control):

EC50= 95 [mg/L]

Applicant's summary and conclusion

Conclusions:

Testing of toxicity to microorganism of Isodecylethernitril yielded a EC50=2514mg/L.

Executive summary:

Testing of toxicity to microorganism of Isodecylethernitril yielded a EC50=2514mg/L.