2-Propenoic acid, 2-isocyanatoethyl ester

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08 Apr 2010 – 18 May 2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Version / remarks:

1984

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

482-140-6

EC Name:

-

Cas Number:

13641-96-8

Molecular formula:

Hill formula: C6 H7 N O3 CAS formula: C6 H7 N O3

IUPAC Name:

2-isocyanatoethyl prop-2-enoate

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil (if used): The test substance was directly added into the soil. A total amount of 10 g mixture of the test substance and dry soil was prepared firstly and then mixed with 490 g dry weight of soil for each test container. Then the moisture content was adjusted at about 35% dry soil weight by adding appropriate volume deionized water.

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: Eisenia fetida
- Source: The worms were obtained from an earthworm nursery of Nanjing city in China.
- Age at test initiation (mean and range, SD): Worms for this study were adult (two months old with clitellum).
- Weight at test initiation (mean and range, SD): 300 mg to 600 mg.

ACCLIMATION
- Acclimation period: Before the test, the worms were acclimated in no polluted soil for 7 days.

Study design

Study type:

laboratory study

Substrate type:

natural soil

Limit test:

no

Total exposure duration:

14 d

Test conditions

Test temperature:

20 °C ± 2 °C

pH:

pH (soil:water = 1:2:5): 8.45 (Jilin black soil); 5.29 (Jiangxi red soil)

Moisture:

28 - 35%

Details on test conditions:

TEST SYSTEM
- Test container (material, size): Glass spotless beakers (1L, Made by Jinzheng Instrument Factory, Nanjing)
- Amount of soil or substrate: 500 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Two types of representative soil collected from Jilin and Jiangxi were chosen to be the natural test soil.
- Soil taxonomic classification: clay (Jilin black soil); clay (Jiangxi red soil)
- Organic matter (g/kg): 28.7 (Jilin black soil); 9.94 (Jiangxi red soil)
- CEC (cmol(+)/kg): 29.8 (Jilin black soil); 10.6 (Jiangxi red soil)
- pH (soil:water = 1:2:5): 8.45 (Jilin black soil); 5.29 (Jiangxi red soil)
- Pretreatment of soil: Before use the soil were air-dried and sieved with a 5-mm mesh screen.

OTHER TEST CONDITIONS
- Photoperiod: Continuous light
- Light intensity: Intensity of 400 lux to 800 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
Mortality of earthworms was assessed on day 7 and day 14 after application.

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 10, 100, 1000 mg/kg soil (Jilin black soil) ; 10, 100, 1000 mg/kg (Jiangxi red soil)
- Results used to determine the conditions for the definitive study: Jinlin black soil: 8 of 10 earthworms were observed dead at a test concentration of 1000 mg/Kg after 14 days. Jiangxi red soil: 10 of 10 earthworms were observed dead at a concentration of 100 mg/kg after 14 days.

Nominal and measured concentrations:

Nominal: Jinlin black soil: 400, 500, 600, 800 and 1000 mg/kg (dry weight of soil); Jiangxi red soil: 30, 50, 80, 100 and 200 mg/kg (dry weight of soil)

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

7 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other:

Remarks:

Jilin Black Soil

Effect concentrations 2

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

928 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other:

Remarks:

Jilin Black Soil

Effect concentrations 3

Duration:

7 d

Dose descriptor:

LC50

Effect conc.:

> 200 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other:

Remarks:

Jiangxi Red Soil

Effect concentrations 4

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

53.5 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other:

Remarks:

Jiangxi Red Soil

Details on results:

- Mortality at end of exposure period: During the test, all earthworms remained in the soil and no obvious physical or pathological symptoms or distinct changes were observed in behaviours of organisms of the control. In Jilin black soil, on the 14th day one to two earthworms died in the treatment with 800 mg/kg soil and six to eight earthworms died in the treatment with 1000 mg/kg soil.·
In Jiangxi red soil, on the 14th day one to two earthworms died in the treatment with 30 mg/kg soil. Greater mortality was observed in the treatment with higher testing concentration and for the concentration of 100 and 200 mg/kg soil all earthworms died. The results showed that the 14 d LC50
of the test substance was 928 mg/kg soil in Jilin Black soil (with a 95% confidence limit of 861-1001 mg/kg soil) and 53.5 mg/kg soil (with a 95% confidence limit of 42.2-67.7 mg/kg soil) in Jiangxi Red soil.
- Behavioural abnormalities: No unusual circumstances that might have affected the integrity and quality of the study were observed.

Results with reference substance (positive control):

No reference substance was used

Any other information on results incl. tables

Table 1: Effect of AOI on Earthworms Observed in the Definitive Test (Jilin Black Soil)

 

Conc. (mg/kg soil)	Number of the test worms	Number of the dead
		7 d	14 d
0	10	0	0
0	10	0	0
0	10	0	0
0	10	0	0
400	10	0	0
400	10	0	0
400	10	0	0
400	10	0	0
500	10	0	0
500	10	0	0
500	10	0	0
500	10	0	0
600	10	0	0
600	10	0	0
600	10	0	0
600	10	0	0
800	10	0	1
800	10	0	2
800	10	0	2
800	10	0	1
1000	10	0	6
1000	10	1	8
1000	10	1	7
1000	10	0	7
LC50 (mg/kg soil)		>1000	928
95% confidence limit (mg/kg soil)		-	861 - 1001

 

 

 

Table 2: Effect of AOI on Earthworms Observed in the Definitive Test (Jilin Black Soil)

 

Conc. (mg/kg soil)	Number of the test worms	Number of the dead
		7 d	14 d
0	10	0	0
0	10	0	0
0	10	0	0
0	10	0	0
30	10	0	1
30	10	0	1
30	10	0	2
30	10	0	1
50	10	0	5
50	10	0	5
50	10	0	7
50	10	0	6
80	10	0	7
80	10	0	7
80	10	0	8
80	10	0	8
100	10	1	10
100	10	0	10
100	10	1	10
100	10	0	10
200	10	1	10
200	10	1	10
200	10	1	10
200	10	1	10
LC50 (mg/kg soil)		>200	53.5
95% confidence limit (mg/kg soil)		-	42.2 – 67.7

 

 

 

Table 3: Validity criteria for OECD 207 (1984)

 

Criterion	Outcome	Criterion fulfilled
Mortality in the control≤10 % at the end of the test	Mortality was not observed in the blank control at the end of the study.	yes

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

See Table 3 in "Any other information on results incl. tables"

Conclusions:

The 14 d LC50 of AOI to earthworm was 928 mg/kg soil in Jilin Black soil (with a 95% confidence limit of 861 - 1001 mg/kg soil) and 53.5 mg/kg soil (with a 95% confidence limit of 42.2 - 67.7 mg/kg soil) in Jiangxi Red soil.