Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 266-257-8 | CAS number: 66215-27-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1980/11 to 1980/12
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- GLP compliance:
- yes
Test material
- Reference substance name:
- N-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- EC Number:
- 266-257-8
- EC Name:
- N-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- Cas Number:
- 66215-27-8
- Molecular formula:
- C6H10N6
- IUPAC Name:
- N2-cyclopropyl-1,3,5-triazine-2,4,6-triamine
Constituent 1
- Radiolabelling:
- yes
- Remarks:
- triazine-ring-labelled 14C-cyromazine (5.5 millcuries; specific activity of 50.3 microcuries per milligram, µCi/mg)
Sampling and analysis
- Details on sampling:
- Water samples were taken on days 0, 3, 7, 10, 14, 21 and 28 of exposure and 1, 3, 7, 10 and 14 of depuration. Four fish were removed from each treatment aquarium, eviscerated and filleted on days 1, 3, 7, 10, 14, 21 and 28 of exposure and days 1, 3, 7, 10 and 14 of depuration and the residues in edible, non-edible and thus also whole fish parts determined. Samples were pooled frozen and homogenized before three aliquots per treatment day were selected for radiometric analysis. The samples were combusted with the resultant 14CO2 being trapped in scintillation fluid, which was placed into a scintillation vial, in a scintillation counter and the counts per minute was determined.
Test solutions
- Vehicle:
- yes
- Remarks:
- Methanol
- Details on preparation of test solutions, spiked fish food or sediment:
- The treatment aquarium (unit 1) was filled with diluent water (75 L) and spiked with 5.16 mL of the 14.53 mg/mL cyromazine diluter stock. The resulting solution was vigorously stirred to achieve homogeneity.
Test organisms
- Test organisms (species):
- Lepomis macrochirus
- Details on test organisms:
- 150 bluegill sunfish with a mean wet weight of 2.5 g and a standard length of 5.7 cm
Study design
- Route of exposure:
- aqueous
- Justification for method:
- aqueous exposure method used for following reason: Standard methodology for bioconcentration determination
- Test type:
- flow-through
- Water / sediment media type:
- natural water
- Total exposure / uptake duration:
- 28 d
- Total depuration duration:
- 7 d
Test conditions
- Test temperature:
- 22 ± 1.0 °C :
For the static test:
21.0 ± 1.0°C - pH:
- for main test 5-7
: For static test 6-7 - Dissolved oxygen:
- 60 % saturation (5.3 mg/mL)
- Details on test conditions:
- The test chambers consisted of a 170 L glass aquarium containing 75 litres of water (treatment chamber) and a 54-litre aquarium containing approximately 30 litres of water (control chamber). The flow through system was calibrated such that the treatment aquarium had a turnover rate of 3 volumes per day, whilst the smaller control aquarium had a turnover rate of seven volumes per day. As the water flowed into the aquaria either the radioactive test treatment or the methanol (solvent) control was added via a chemical induction system.
- Nominal and measured concentrations:
- 1.0 mg/mL
Results and discussion
- Details on results:
- The maximum accumulation of 14C-residues in the edible portion (muscle) of the fish occurred on day three of exposure. After day three the rate of elimination was far greater than the rate of accumulation. Based on a mean maximum concentration of 0.67 ± 0.021 µg/g measured in the edible tissue for day 3 and a mean concentration of 0.93 ± 0.22 µg/mL 14C-cyromazine measured in the water during day 0-3 of exposure, the mean bioconcentration factor of 14C-cyromazine in bluegill edible tissue was less than 1x.
In non-edible tissues (viscera-carcass) the concentration of 14C-cyromazine in continuously exposed tissue occurred during the first day of exposure. Based on the mean concentrations of 2.1 ± 0 1 µg/g measured in non-edible tissues (day 1) and mean exposure concentration of 0.84 ± 0.23 µg/mL (days 0-1), the maximum bioconcentration factor for 14C-cyromazine in the non-edible bluegill tissues was 2.5x. Following a continuous decrease of 14C-residues from the non-edible tissue 1-14 days of exposure from days 14-28 an apparent period of equilibrium was established. Therefore, the mean equilibrium bioconcentration factor for 14C-cyromazine observed throughout the observation period was less than 1 in the non-edible bluegill tissues. Analyses were also performed to estimate the total 14C-residue content on a whole fish basis.
The maximum bioconcentration factor for 14C-cyromazine for the whole fish was less than 1. The level of 14C-cyromazine in edible fish portions was below detectable levels throughout the depuration phase. The half-life of 14C-residues in the edible tissue occurred during exposure days 3-7. For non-edible tissues 14C-residues were below the limit of detection by day 10 of the depuration phase. The half-life for 14C-residues in non-edible tissues occurred between days 3 and 7 of depuration. The whole body 14C-residues were below the limit of detection throughout the depuration phase and was directly related to the lack of measurable concentrations in the edible portions
As in the 3-d flow-through exposure period the maximum accumulation of 14C residues in bluegill exposed to 14C-cyromazine for 28-days occurred within the first 3-days, it was necessary to re-initiate the dynamic flow-through to obtain fish for us in the the static exposure test. The dynamic flow-through system was re-employed for 3-days to obtain fish for use in the 4-day static exposure test although the aqueous 14C-concentrations were equivalent to those in the flow-through system the actual measured 14C residues in the tissues were only 24-30% of those, which had been previously observed in this main 28-day exposure test.
During the 4-day static exposure, fish exhibited normal behaviour and were in good condition The mean water temperature was 21 ± 1°C, the dissolved oxygen concentration ranged from 4.0-8.6 mg/L (44-96% of saturation) during the test with the pH being 6.6-7.1
The bioconcentration factors in the muscle, viscera, carcass and whole body were all less than 1.
Any other information on results incl. tables
Table 1
Measured 14C-residue concentrations of cyromazine in tissues of bluegill sunfish during exposure for 28 days under flow-through conditions (1.0 mg cyromazine/L) followed by 14-day depuration phase
Period | Day | Measured water conc (µg/mL)a | 14C-residue concentrations (µ /g) | ||
Edible b | Non-edible b | Whole fish c | |||
Exposure | 0 | 1.0 (0.058) | - | - |
|
1 | 1.1 (0) | < 0.18 d | 2.1 | <0.62 e | |
3 | 1.1 (0) | 0.67 | 0.72 | 0.70 | |
7 | 1.1 (0) | < 0.15 d | 0.51 | < 0.29 e | |
10 | 1.0 (0.058) | 0.12 | 0.44 | 0.26 | |
14 | 1.0 (0.058) | 0.081 | 0.27 | 0.15 | |
21 | 1.0 (0) | < 0.20 d | 0.34 | < 0.25 e | |
28 | 1.0 (0.058) | < 0.17 d | 0.35 | < 0.24 e | |
Depuration | 1 | 0.019 (0.0049) | < 0.26 d | 0.38 | <0.31 e |
3 | 0.023 (0.0061) | < 0.23 d | 0.21 | <0.22 e | |
7 | 0.011 (0.0058) | < 0.14 d | 0.16 | < 0.15 d | |
10 | <0.0078 d | <0.15 d | < 0.28 d | < 0.21 d | |
14 | 0.0098 | <0.19 d | < 0.17 d | < 0.17 d |
a Mean and standard deviation based on radiometric analysis of triplicate water samples
b Mean and standard deviation based on the radiometric analysis of triplicate samples of the pooled tissue homogenate from four fish
c Concentration based on the summation of the mean measured concentration of 14C-residues from each tissue portion and the total weight of the tissue portion from four fish
d Concentration below minimum detectable levels
e Concentration of one portion below minimum detectable levels
Table 2
Measured 14C-residue concentrations of cyromazine in fish tissues after 4-days of static exposure
Mean measured conc (µg/mL) | 14C-residue concentrations (µg/g) | |||
Water | Muscle | Viscera | Carcass | Whole body |
1 3 ± 0 045 | 0.057 (0.0015) | 0.10 (0.00058) | 0.15 (0) | 0.090 (-) |
Standard deviation in brackets
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- Data generated in the flow-through and static test systems 14C-cyromazine shows no potential to bio- accumulate in bluegill sunfish. The mean equilibrium bioconcentration factor for 14C-cyromazine observed throughout the observation period was less than 1.
- Executive summary:
The objective of this investigation was to define the kinetics of the uptake and elimination of cyromazine, an experimental insect growth inhibititor, including its degradation products and metabolites by bluegill sunfish, in a dynamic flow thorugh system. Bluegill sunfish were continously exposed to a mean measured concentration of 1.0 ± 0.14 μg/mL cyromazine in well water for 28 days after which the remaining fish transferred to flowing, uncontaminated water for a 14 depuration period. Based on mean maximum concentrations in the edible tissue for day 3 and the mean concentration measured in the water during day 0-3 of exposure, the mean bioconcentration factor of 14C-cyromazine in bluegill edible tissue was less than 1x. The maximum bioconcentration factor for 14C-cyromazine in the non-edible bluegill tissues was 2.5x. The mean equilibrium bioconcentration factor for 14C-cyromazine observed throughout the observation period was less than 1 in the non-edible bluegill tissues. The maximum bioconcentration factor for 14C-cyromazine for the whole fish was less than 1. The level of 14C-cyromazine in edible fish portions was below detectable levels throughout the depuration phase. The half-life for 14C-residues in non-edible tissues occurred between days 3 and 7 of depuration. The whole body 14C-residues were below the limit of detection throughout the depuration phase and was directly related to the lack of measurable concentrations in the edible portions.
A 4-day static test was also performed for the purpose of determining the metabolism of cyromazine by bluegill sunfish. The test duration was based on the accumulation, persistence and elimination of cyromazine observed in the 28-day exposure test. The bioconcentration factors in the muscle, viscera, carcass and whole body were all less than 1.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.