Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Water solubility
Administrative data
Link to relevant study record(s)
- Endpoint:
- water solubility
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 105 (Water Solubility)
- GLP compliance:
- no
- Type of method:
- flask method
- Specific details on test material used for the study:
- Lot PF 897
- Key result
- Water solubility:
- > 538 g/L
- Conc. based on:
- test mat.
- Temp.:
- 30 °C
- pH:
- ca. 7
- Remarks on result:
- other: The pH was not reported and was assumed to be 7
- Details on results:
- The highest concentration of ω-Transaminase in water is achieved at 538 mg/mL. At tis concentration, the solution is in a gel-like state. Adding additional lyophilized powder into this solution will make mixing impossible.
- Conclusions:
- ω-Transaminase is extremely soluble in water. It was not possible to measure the full extent of ω-Transaminase solubility as the solution becomes too viscous above 538 mg/mL and it cannot be mixed sufficiently to confirm all of the lyophilized powder was dissolved. Therefore, the water solubility of ω-Transaminase is reported as > 538 g/L.
Reference
The final concentration of ω-Transaminase is calculated based on the amount of lyophilized powder added and the final volume of the solution (Table 1). The UV280nm absorbance of each solution is measured to confirm that the OD is within linear range.
Table 1. Final volume and concentration of ω-Transaminase in water
Weight of ω-Transaminase (mg) | Water (mL) | Total Volume (mL) |
63.1 | 5.00 | 5.10 |
108.2 | 5.00 | 5.24 |
201.5 | 5.00 | 5.34 |
411.4 | 5.00 | 5.46 |
614.8 | 5.00 | 5.77 |
807.6 | 5.00 | 5.90 |
1508.8 | 5.00 | 6.28 |
2415.2 | 5.00 | 6.30 |
3522.0 | 5.00 | 6.54 |
4541.9 | 5.00 | 6.62 |
Description of key information
ω-Transaminase is extremely soluble in water. It was not possible to measure the full extent of ω-Transaminase as the solution becomes too viscous above 538 mg/mL and it cannot be mixed sufficiently to confirm all of the lyophilized powder was dissolved. Therefore, the water solubility of ω-Transaminase is reported as > 538 g/L.
Key value for chemical safety assessment
- Water solubility:
- 538 g/L
- at the temperature of:
- 30 °C
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.