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Reaction mass of Disodium [3-{[4-chloro-2-(hydroxy-kO)-5-methoxyphenyl]diazenyl-kN2}-4-(hydroxy-kO)naphthalene-2,7-disulfonato(4-)](hydroxy)chromate(2-) and Trisodium [3-{[4-chloro-2-(hydroxy-kO)-5-methoxyphenyl]diazenyl-kN2}-4-(hydroxy-kO)naphthalene-2,7-disulfonato(4-)](dihydroxy)chromate(3-)
EC number: 953-115-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 December 1994 to 09 November 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Code number: FAT-20044/B
Batch-Nr.: 99
Appearance: solid
Solubility: 60g/l [in water]
Storage: room temperature
Expiration date: 07/99 - Analytical monitoring:
- yes
- Details on sampling:
- Test medium samples for the analytical determinations were taken from the approximate center of the test vessels ( after mixing the medium ) and kept at -18 °C to -25 °C until analysis. The samples of the test substance at the beginning and at the end of the test were sent to the PE Analytical Services K, Textile Dyes Division.
- Vehicle:
- yes
- Remarks:
- Tween 80 (Polyoxyethylensorbitanmonooleat)
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The organism used in this test system is the species Daphnia magna. The daphnids were bred in the laboratories of the Product Ecology TF, Textile Dyes Division under standardised conditions.
Breeding:
- Cultures of Daphnia magna are held in glass vessels containing approx. 3.0 litre of reconstituted water at 18 - 22 °C. The water was renewed partially three times per week. The daphnids were fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a TETRAMIN-extract in such quantities that the food was consumed within 24h.
Pretreatment:
- 24 hours before the start of the exposure reproductive daphnids were separated from the young daphnids by sieving all individuals through a 1000 µm sieve.
For each test concentration, 20 daphnids divided in two groups of 10 animals were used. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 178 mg/l CaCO3
- Test temperature:
- 21±1 °C
- pH:
- 7.9 to 8
- Dissolved oxygen:
- 95 to 98
- Nominal and measured concentrations:
- Nominal: 45, 100, 220 mg/l
Measured:
Actual mean conc, mg/l: 47, 105 and 225 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichmmate.
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 225 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 225 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- No immobilization and abnormal behaviour of the Daphnia was observed in the controls and in the test concentrations.
- Results with reference substance (positive control):
- The EC50 value should be in the range of 0.8-1.5mg/l. Last reference control at October 12, 1994 ; EC50 1.01 mg/l.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC and EC50 of FAT 20044/B after 48 hours was 225 mg/l.
- Executive summary:
The short term toxicity to daphnids was carried out with FAT 20044/B according to OECD guideline 202. A range finding test was carried out at concentrations from 0.01 - 100 mg/l to determine the concentrations range for the main test. For the main test, the nominal concentrations tested were 45 / 100 and 220 mg test article/I, a vehicle and a blank control without any additions. The test concentrations were prepared from a 0.220 g/l stock solution. In all test concentrations no visible alteration of the test substance was observed after 48 hours. During the test the test article concentrations in the analysed test media were in the range of 98 - 108 % of the nominal values. Therefore, under test conditions the test article was sufficiently stable in the test media. The reported results are related to the actual mean values of the analysed concentrations of the test article in the test medium, measured at the beginning and at the end of the test. No immobilization and abnormal behaviour of the daphnids was observed in the controls and in the test concentrations. The EC50 and the EC100 value could not be determined, but are clearly higher than 225 mg/l. Based on the study results, The NOEC and EC50 of FAT 20044/B after 48 hours was 225 mg/l.
Reference
Determination of the EC - values
A range finding test was carried out at concentrations from 0.01 - 100 mg/l to determine the concentrations range for the main test. For the main test the nominal concentrations tested were 45 / 100 and 220 mg test article/I, a vehicle and a blank control without any additions. The test concentrations were prepared from a 0.220 g/l stock solution. In all test concentrations no visible alteration of the test substance was observed after 48 hours. During the test the test article concentrations in the analysed test media were in the range of 98 - 108 % of the nominal values. Therefore, under test conditions the test article was sufficiently stable in the test media. The reported results are related to the actual mean values of the analysed concentrations of the test article in the test medium, measured at the beginning and at the end of the test. No immobilization and abnormal behaviour of the daphnids was observed in the controls and in the test concentrations. The NOEC and EC50 value after 48 hours were determined to be 225 mg/l.
The EC50 and the EC-100 value could not be determined, but are clearly higher than 225 mg/l.
Number of immobilized daphnids after 24 and 48 hours
Actual conc. of test substance mg/l | No. of animals | No. of responses | % immobility | ||
24 h | 48 h | 24 h | 48 h | ||
Blank
| 10 | 0 | 0 | 0 | 0 |
10 | 0 | 0 | |||
Vehicle
| 10 | 0 | 0 | 0 | 0 |
10 | 0 | 0 | |||
47 | 10 | 0 | 0 | 0 | 0 |
10 | 0 | 0 | |||
105 | 10 | 0 | 0 | 0 | 0 |
10 | 0 | 0 | |||
225 | 10 | 0 | 0 | 0 | 0 |
10 | 0 | 0 |
Description of key information
The short-term toxicity to daphnids was carried out with FAT 20044/B according to OECD guideline 202. A range finding test was carried out at concentrations from 0.01 - 100 mg/l to determine the concentrations range for the main test. For the main test, the nominal concentrations tested were 45 / 100 and 220 mg test article/I, a vehicle and a blank control without any additions. The test concentrations were prepared from a 0.220 g/l stock solution. In all test concentrations no visible alteration of the test substance was observed after 48 hours. During the test the test article concentrations in the analysed test media were in the range of 98 - 108 % of the nominal values. Therefore, under test conditions the test article was sufficiently stable in the test media. The reported results are related to the actual mean values of the analysed concentrations of the test article in the test medium, measured at the beginning and at the end of the test. No immobilization and abnormal behaviour of the daphnids was observed in the controls and in the test concentrations. The EC50 and the EC100 value could not be determined, but are clearly higher than 225 mg/l. Based on the study results, the NOEC and EC50 of FAT 20044/B after 48 hours was 225 mg/l.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 225 mg/L
Additional information
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