Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 271-587-0 | CAS number: 68585-78-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP-compliant, guideline study with some deviations.
- Justification for type of information:
- Read across valid as the substnace tested is also titanate complex with alkylphosphate, degrading in water to form simple alcohols including 2-propanol and phosphate esters.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Principles of method if other than guideline:
- Due to the rapid hydrolysis of the test substance on contact with water, the original substance was not analysed in test solutions and it could not be verified that substances were maintained within 80% of nominal concentrations.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No data reported. - Analytical monitoring:
- no
- Details on sampling:
- Growth measurements were made at 0, 24, 48 and 72 hours.
- Vehicle:
- yes
- Details on test solutions:
- A master culture was used to innoculate the sterile nutrient medium. The inoculated solution was incubated at 24°C and stirred continuously using an orbital shaker under continuous illumination of c. 7000 lux. Auxillary solvent was used to dissolve 200 mg of test substance. This was diluted to required concentrations and added to algal suspensions.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Species: CCAP 276/20. Holding conditions: on orbital shaker under continuous illumination for 72 hours (OECD guideline 201). Temperature: 24 +/-1°C. Source: Culture Centre of Algae and Protozoa c/o Freshwater Biological Association, Cumbria, UK.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- No data reported.
- Hardness:
- No data reported.
- Test temperature:
- 24 +/- 1°C
- pH:
- The pH ranged from 7.9-8.0 at test initiation and from 7.9 to 9.1 at test termination.
- Dissolved oxygen:
- Not aerated. Orbital shaker was used to maintain the gaseous exchange.
- Salinity:
- Freshwater
- Nominal and measured concentrations:
- Nominal concentrations: 0.125, 0.25, 0.5, 1.0 and 2.0 mg/L.
- Details on test conditions:
- Vessels: 100 ml of test solution was placed in loosely stoppered 250 ml glass flasks. Number of concentrations: 5 test solutions, 1 solvent control (100 ml/L auxillary solvent), 1 blank control. Number of replicates per concentration: 3. Illumination: continuous at c. 700 lux.
- Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.45 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.125 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- All solutions were visually inspected at test termination and no abnormalities were detected.
- Results with reference substance (positive control):
- Mean cell density of control: 1.06E-05 cells/ml at test initiation and 4.86E+06 cells/ml at test termination. Mean cell density of solvent control: 1.17E+05 at test initiation and 4.93E+06 cells/ml at test termination.
- Reported statistics and error estimates:
- The index of growth was taken from the area under each curve. Comparison of the area under the test curve with the area under the solvent curve was used to determine the inhibition of growth at each concentration. The straight section of the graph was used to determine the average maximum growth rate for each solution.
- Validity criteria fulfilled:
- no
- Remarks:
- Due to the rapid hydrolysis of the test substance on contact with water, the original substance was not analysed in test solutions and it could not be verified that substances were maintained within 80% of nominal concentrations.
- Conclusions:
- The 72 hour EC50 for biomass is 1.45 mg/L. The 24-48 hour EC50 for growth rate is c. 2 mg/L. The 72 hour NOEC is 0.125 mg/L.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20-23 September 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP-compliant, guideline study with some deviations.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- Due to the rapid hydrolysis of the test substance on contact with water, the original substance was not analysed in test solutions and it could not be verified that substances were maintained within 80% of nominal concentrations.
- Principles of method if other than guideline:
- Due to the rapid hydrolysis of the test substance on contact with water, the original substance was not analysed in test solutions and it could not be verified that substances were maintained within 80% of nominal concentrations.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No data reported. - Analytical monitoring:
- no
- Details on sampling:
- Growth measurements were made at 0, 24, 48 and 72 hours.
- Vehicle:
- yes
- Details on test solutions:
- A master culture was used to innoculate the sterile nutrient medium. The inoculated solution was incubated at 24°C and stirred continuously using an orbital shaker under continuous illumination of c. 7000 lux. Auxillary solvent was used to dissolve 200 mg of test substance. This was diluted to required concentrations and added to algal suspensions.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Species: CCAP 276/20. Holding conditions: on orbital shaker under continuous illumination for 72 hours (OECD guideline 201). Temperature: 24 +/-1°C. Source: Culture Centre of Algae and Protozoa c/o Freshwater Biological Association, Cumbria, UK.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- No data reported.
- Hardness:
- No data reported.
- Test temperature:
- 24 +/- 1°C
- pH:
- The pH ranged from 7.9-8.0 at test initiation and from 7.9 to 9.1 at test termination.
- Dissolved oxygen:
- Not aerated. Orbital shaker was used to maintain the gaseous exchange.
- Salinity:
- Freshwater
- Nominal and measured concentrations:
- Nominal concentrations: 0.125, 0.25, 0.5, 1.0 and 2.0 mg/L.
- Details on test conditions:
- Vessels: 100 ml of test solution was placed in loosely stoppered 250 ml glass flasks. Number of concentrations: 5 test solutions, 1 solvent control (100 ml/L auxillary solvent), 1 blank control. Number of replicates per concentration: 3. Illumination: continuous at c. 700 lux.
- Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.45 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.125 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- All solutions were visually inspected at test termination and no abnormalities were detected.
- Results with reference substance (positive control):
- Mean cell density of control: 1.06E-05 cells/ml at test initiation and 4.86E+06 cells/ml at test termination. Mean cell density of solvent control: 1.17E+05 at test initiation and 4.93E+06 cells/ml at test termination.
- Reported statistics and error estimates:
- The index of growth was taken from the area under each curve. Comparison of the area under the test curve with the area under the solvent curve was used to determine the inhibition of growth at each concentration. The straight section of the graph was used to determine the average maximum growth rate for each solution.
- Validity criteria fulfilled:
- no
- Remarks:
- Due to the rapid hydrolysis of the test substance on contact with water, the original substance was not analysed in test solutions and it could not be verified that substances were maintained within 80% of nominal concentrations.
- Conclusions:
- The 72 hour EC50 for biomass is 1.45 mg/L. The 24-48 hour EC50 for growth rate is c. 2 mg/L. The 72 hour NOEC is 0.125 mg/L.
- Executive summary:
Douglas (1991) is a GLP-compliant, acute toxicity study on algae following OECD guideline 201. Due to the rapid hydrolysis of the test substance on contact with water, the original substance was not analysed in test solutions and it could not be verified that substances were maintained within 80% of nominal concentrations. The vessels were loosely stoppered to prevent evaporation and the study is considered reliable and suitable for use for this endpoint.
Referenceopen allclose all
Description of key information
No study was conducted on the target substance. Read-across from structural analogue, Titanium butyl phosphate, is valid in that both substances are hydrolytically unstable and forms simple alcohols, including 2-propanol, and phosphate esters.
Based on the results of the structural analogue, the 72 hour EC50 for biomass is 1.45 mg/L. The 24-48 hour EC50 for growth rate is c. 2 mg/L. The 72 hour NOEC is 0.125 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1.45 mg/L
- EC10 or NOEC for freshwater algae:
- 0.125 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.