Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 949-711-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_environmental-fate-and-pathways.png)
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 5 January 2018 to 11 April 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
- Version / remarks:
- Adopted March 23, 2006 and corrected September 26, 2014
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Saccharomyces cerevisiae, lysate
- EC Number:
- 305-230-8
- EC Name:
- Saccharomyces cerevisiae, lysate
- Cas Number:
- 94350-12-6
- IUPAC Name:
- Saccharomyces cerevisiae, lysate
- Test material form:
- solid: particulate/powder
- Remarks:
- light beige
- Details on test material:
- - Source and lot/batch No.of test material:
supplied by the sponsor, batch no. AC17F00560
- Expiration date of the lot/batch: February 2019
- Purity test date: 30 June 2017
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted.
The solid material was re-suspended in test water and centrifuged again. This procedure was done three times. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined.
Based on this ratio, calculated aliquots of washed sludge suspension were mixed with test water corresponding to 4 g dry material per litre and aerated with CO2 free air overnight.
Initial test substance concentration
- Initial conc.:
- 20.082 mg/L
- Based on:
- TOC
- Remarks:
- This corresponds to 43.92 mg/L test item based on an organic carbon content of 0.4272 mg carbon/ mg test item.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: reconstituted test water
- Additional substrate: no
- Solubilising agent (type and concentration if used): no
- Test temperature: 20°C ± 1°C
- pH: 7.6
- pH adjusted: no
- CEC (meq/100 g): not reported
- Aeration of dilution water: the test water was aerated with CO2-free air prior to test start
- Suspended solids concentration: not reported
- Continuous darkness: yes
TEST SYSTEM
- Glass bottles with screw-caps and Teflon-coated septa (125 mL nominal, 128 mL total volume)
- 3 replicates per treatment and per sampling time. For the day 28, 5 replicates per treatment
For the day 2, only 2 replicates were analysed (damage of one flask)
- FT : test item (20.082 mg carbon/L) + activated sludge and test water
FB : Inoculum control with activated sludge and test water
Fc : procedure control with the reference item, sodium benzoate (19.976 mg Carbon/L), activated sludge and test water
Fi : Toxicity control (20.188 mg carbon/L) with test item , reference item (sodium benzoate), activated sludge and test water
Sodium hydroxyde solution 7M : two additional flasks with Sodium hydroxide solution (in CO2 free pure water) were prepared at each sampling date, except at day 2. The measured inorganic carbon content of the test flasks were corrected by this blank value. Additionally, two flasks with test water were measured at each sampling date to ensure that the water is nearly free of carbon.
- Sampling intervals : FT and FB: days 0, 2, 5, 9, 14, 16, 21, 28
FC and FI: days 0, 5, 14, 28
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 68.3
- Sampling time:
- 16 d
- Remarks on result:
- other: readily biodegradable
- Details on results:
- The 10-day window started at day 2, when the degradation was 17.1%. At sampling at day 9 (within the 10-day window), the degradation was 66.8%. Therefore, the ready biodegradation criterion was passed.
The degree of biodegradation of the test item is 68.3% in 16 days. At the end of the test at day 28, the degradation was 56.4%.
In the toxicity control containing both, the test item and the reference item sodium benzoate, 70.0% biodegradation was noted within 14 days and 58.1% biodegradation after 28 days of incubation. Therefore the test item is not inhibitory.
BOD5 / COD results
- Results with reference substance:
- The reference item sodium benzoate was sufficiently degraded to 74.5% after 14 days and to 64.0% after 28 days of incubation.
Any other information on results incl. tables
TIC produced for the Test Item, Reference Item and Toxicity Control during the Test Period of 28 Day
Treatment |
Carbon Content (mg/L)
|
Replicate |
Day 0 2 5 9 14 16 18 28 ICcorrected(mg/L)1 |
|||||||
Na-Benzoate
|
19.976
|
1 |
0.00 |
- |
15.88 |
- |
14.04 |
- |
- |
13.45 |
2 |
0.00 |
- |
12.97 |
- |
15.70 |
- |
- |
12.80 |
||
3 |
0.00 |
- |
12.26 |
- |
** |
- |
- |
11.60 |
||
4 |
- |
- |
- |
- |
- |
- |
- |
13.11 |
||
5 |
- |
- |
- |
- |
- |
- |
- |
12.91 |
||
Mean |
0.00 |
|
13.70 |
|
14.87 |
|
|
12.77 |
||
Saccharomyces cerevisiae, lysate
|
20.082
|
1 |
0.77 |
3.43 |
7.92 |
13.83 |
13.23 |
12.87 |
10.69 |
5.50 |
2 |
1.00 |
3.43 |
6.48 |
13.04 |
14.03 |
13.75 |
11.28 |
10.00 |
||
3 |
0.89 |
*** |
3.62 |
13.35 |
13.68 |
14.54 |
13.27 |
11.66 |
||
4 |
- |
- |
- |
- |
- |
- |
- |
13.78 |
||
5 |
- |
- |
- |
- |
- |
- |
- |
15.72 |
||
Mean |
0.89 |
3.43 |
6.00 |
13.41 |
|
|
11.75 |
11.33 |
||
Toxicity control |
20.188 |
1 |
0.00 |
- |
19.35 |
- |
12.94 |
- |
- |
14.10 |
2 |
0.00 |
- |
13.93 |
- |
14.27 |
- |
- |
9.53 |
||
3 |
0.00 |
- |
0.20 |
- |
15.16 |
- |
- |
8.78 |
||
4 |
- |
- |
- |
- |
- |
- |
- |
16.33 |
||
5 |
- |
- |
- |
- |
- |
- |
- |
9.86 |
||
Mean |
0.00 |
|
11.16 |
|
14.12 |
|
|
11.72 |
1 Corrected by the inoculum control
- Not applicable
** considered as outlier, value not used for calculation
*** no measurement possible due to damage of test flask
Biodegradation of Test Item, Reference Item and Toxicity Control during the Test Period of 28 Day
Treatment |
Carbon Content (mg/L) |
Replicate |
Day 0 2 5 9 14 16 21 28 % biodegradation [net measured TIC/initial C *100] |
|||||||
Na-Benzoate
|
19.976 |
1 |
0.00 |
- |
79.5 |
- |
70.3 |
- |
- |
67.4 |
2 |
0.00 |
- |
64.9 |
- |
78.6 |
- |
- |
64.1 |
||
3 |
0.00 |
- |
61.4 |
- |
** |
- |
- |
58.1 |
||
4 |
- |
- |
- |
- |
- |
- |
- |
65.6 |
||
5 |
- |
- |
- |
- |
- |
- |
- |
64.6 |
||
Mean |
0.00 |
|
68.6 |
|
74.5 |
|
|
64 |
||
Saccharomyces cerevisiae, lysate |
20.082 |
1 |
3.8 |
17.1 |
39.4 |
68.9 |
65.9 |
64.1 |
53.2 |
27.4 |
2 |
5.0 |
17.1 |
32.2 |
65.0 |
69.8 |
68.4 |
56.2 |
49.8 |
||
3 |
4.4 |
*** |
18.0 |
66.5 |
68.1 |
72.4 |
66.1 |
58.1 |
||
4 |
- |
- |
- |
- |
- |
- |
- |
68.6 |
||
5 |
- |
- |
- |
- |
- |
- |
- |
78.3 |
||
Mean |
4.4 |
17.1 |
29.9 |
66.8 |
67.9 |
68.3 |
58.5 |
56.4 |
||
Toxicity control |
20.188 |
1 |
0.0 |
- |
95.8 |
- |
64.1 |
- |
- |
69.9 |
2 |
0.0 |
- |
69.0 |
- |
70.7 |
- |
- |
47.2 |
||
3 |
0.0 |
- |
1.0 |
- |
75.1 |
- |
- |
43.5 |
||
4 |
- |
- |
- |
- |
- |
- |
- |
80.9 |
||
5 |
- |
- |
- |
- |
- |
- |
- |
48.9 |
||
Mean |
0.0 |
|
55.3 |
|
70.0 |
|
|
58.1 |
- Not applicable
** considered as outlier, value not used for calculation
*** no measurement possible due to damage of test flask
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The biodegradation of Saccharomyces cerevisiae, lysate estimated with the OECD 310 guideline, is 68.3% in 16 days and it satisfies the 10-day window.
- Executive summary:
The substance Saccharomyces cerevisiae, lysate was tested for its ready biodegradability according to the OECD guideline No. 310 (CO2 headspace) and in compliance with the GLP.
The Inoculum was activated sludge microorganisms supplied by a municipal sewage treatment plant receiving domestic waste water.
The CO2 evolution resulting from the aerobic biodegradation of the test item by the inoculum was determined by measuring the inorganic carbon in the test flasks corrected by the control flasks. The extent of biodegradation was expressed as a percentage of the theoretical maximum IC production, based on the quantity of test substance initially added (as organic carbon). As a reference item, sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
The test consisted of four groups :
FT : test item (20.082 mg carbon/L) + activated sludge and test water,
FB : inoculum control with activated sludge and test water,
Fc : procedure control with the reference item, sodium benzoate (19.976 mg Carbon/L), activated sludge and test water
Fi : toxicity control (20.188 mg carbon/L) with test item, reference item (sodium benzoate), activated sludge and test water
The reference item sodium benzoate was sufficiently degraded to 74.5% after 14 days of incubation, thus confirming the suitability of the aerobic activated sludge inoculum used.
In the toxicity control containing both, the test item and the reference item sodium benzoate, 70.0% biodegradation was noted within 14 days. Therefore it can be assumed that the test item is not inhibitory to the aerobic activated sludge microorganisms.
The biodegradation of the test item reached 68.3% in 16 days.
The 10-day window started at day 2, when the degradation was 17.1%. At day 9 (within the 10-day window), the degradation was 66.8%. Therefore, the ready biodegradation criterion was passed.
In conclusion, Saccharomyces cerevisiae, lysate is designated as readily biodegradable, fulfilling the 10-day window.
This biodegradation test is classified as acceptable, and satisfies the guideline requirements of the OECD 310.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)