4-fluoroaniline

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2002

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

publication

Title:

METABONOMIC ASSESSMENT OF TOXICITY OF 4-FLUOROANILINE, 3,5-DIFLUOROANILINE AND 2-FLUOR0-4-METHYLANILINE TO THE EARTHWORM EISENIA VENETA (ROSA): IDENTIFICATION OF NEW ENDOGENOUS BIOMARKERS

Author:

Jacob G. Bundy

Year:

2002

Bibliographic source:

Environmental Toxicology and Chemistry, Vol 21, No.9, 2002

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

4-fluoroaniline

EC Number:

206-735-5

EC Name:

4-fluoroaniline

Cas Number:

371-40-4

Molecular formula:

C6H6FN

IUPAC Name:

4-fluoroaniline

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples were analyzed on a Broker Avance DRX600 spectrometer at a frequency of 600.13 MHz

Test substrate

Vehicle:

not specified

Details on preparation and application of test substrate:

Fully clitellate adult individuals were selected for bioassay work and allowed to depurate on moist filter paper for 24 h before the start of the test. Medium-weight filter paper was cut to fit inside a glass via! without overlapping. The area of filter paper used was 52 cm2. Tue compound under test was dissolved in hexane and applied to the filter paper to give a nominal concentrationin g/cm2 and the hexane allowed to evaporate. The paper was then moistened with 1 ml of distilled water, a single earthworm added, and the via! sealed. The via! was then incubated in the dark for a period of 72 h.
nitrogen, and stored at 20 C until sample preparation

Test organisms

Test organisms (species):

other: Eisenia veneta

Animal group:

annelids

Details on test organisms:

Earthworms (E. veneta) were taken from a culture maintained
at the Centre for Ecology and Hydrology Monks Wood
(Abbots Ripton, Huntingdon, UK)

Study design

Study type:

laboratory study

Substrate type:

filter paper

Total exposure duration:

72 h

Post exposure observation period:

At the end of the exposure period the earthworm was removed and stored at -20°C until sample preparation

Test conditions

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

0.115 mg/cm²

Basis for effect:

mortality

Applicant's summary and conclusion

Conclusions:

The median lethal conenctration value obtained for 4-fluoroaniline is 0.115 mg/cm²