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EC number: 850-698-3 | CAS number: 2387913-24-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Nanomaterial pour density
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- Endpoint summary
- Stability
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2020-04-02 to 2020-04-24
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- The test item concentrations were weighed out on bent object slides and were transferred in the test vessels. All concentrations were stirred overnight in 200 mL ultrapure water. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: Municipal sewage treatment plant of 31137 Hildesheim, Germany
- Pretreatment: The sludge was washed twice and adjusted with chlorine free tap water if necessary. The sludge was used within 24 h after sampling. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- Nominal: 18 - 22 °C, measured: 21.2 °C without ATU, 20.4 °C with ATU
- pH:
- 7.5 ± 0.5
- Dissolved oxygen:
- Aeration was permanent, to keep the dissolved oxygen concentration above 60 - 70 % saturation and to maintain the sludge flakes in suspension.
6.62 - 8.81 mg/L - Nominal and measured concentrations:
- nominal 1 - 3.16 - 10 - 31.6 - 100 mg a.i./L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1000 mL laboratory bottles with screw cap, ISO 4796
- Aeration: Permanent, to keep the dissolved oxygen concentration above 60 - 70 % saturation and to maintain the sludge flakes in suspension.
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 3 at the beginning of the test, 3 at the end of the test
- No. of vessels per positive control (replicates): 3
- Sludge concentration (weight of dry solids per volume):
Dry solids concentration [g/L] of sludge for test without ATU was 3.09
Dry solid concentration [g/L] in test solution for test without ATU was 1.55
Dry solids concentration [g/L] of sludge for test with ATU was 3.07
Dry solid concentration [g/L] in test solution for test with ATU was1.54
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic waste water
OTHER TEST CONDITIONS
- Adjustment of pH: not required (within prescribed range)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
oxygen uptake rate, 3 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: sqrt(10)
- Range finding study:
- Test concentrations: 1, 10, 100, 1000
- Results used to determine the conditions for the definitive study: -1% inhibition at 1 mg a.i./L, 16% inhibition at 10 mg a.i./L, 91% inhibition at 100 mg a.i./L, 99% inhibition at 1000 mg a.i./L - Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 6.34 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: 95% c.i. 5.43 – 7.38 mg/L
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 26.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: 95% c.i. 24.2 – 28.3 mg/L
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:
- Effect concentrations exceeding solubility of substance in test medium:
- Adsorption (e.g. of test material to the walls of the test container):
- Blank controls oxygen uptake rate:
- Coefficient of variation of oxygen uptake rate in control replicates: - Results with reference substance (positive control):
- - Results with reference substance valid? Ja
- Relevant effect levels: EC50 = 106 mg/L, 95% Confidence Interval = 103 - 109 mg/L
- valid range: 53 - 155 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- The 3 h NOEC of Quarternary ammonium compounds, tri-C8-C10-alkylmethyl, Me sulfates is 3.16 mg a.i./L. The 3 h EC10 is 6.34 mg a.i./L, and the EC50 is 26.1 mg a.i./L.
- Executive summary:
In a 3 hour toxicity study conducted according to OECD Guideline 209 (2010), the cultures of activated sludge of a predominantly domestic sewage treatment plant were exposed to Quarternary ammonium compounds, tri-C8-C10-alkylmethyl, Me sulfates at nominal concentrations of 0 (control), 1, 3.16, 10, 31.6, and 100 mg a.i./L mg/L under static conditions.
The batch of activated sludge was checked for sensitivity by testing the reference substance Copper (II) sulphate pentahydrate.
The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the controI respiration rates. The mean inhibition of respiration for the test item replicates ranged from -1% to 87%
The 3 h NOEC of Quarternary ammonium compounds, tri-C8-C10-alkylmethyl, Me sulfates is 3.16 mg a.i./L. The 3 h EC10 is 6.34 mg a.i./L, and the EC50 is 26.1 mg a.i./L.
Results Synopsis
Test Organism: activated sludge from a sewage treatment plant treating predominantly domestic sewage
Test Type: Static
3 h NOEC: 3.16 mg/L
3 h EC10: 6.34 mg/L, 95% c.i. 5.43 - 7.38 mg/L
3 h EC50: 26.1 mg/L, 95% c.i. 24.2 – 28.3 mg/L
Endpoint effected: Inhibition of respiration
Reference
Oxygen Uptake Rates and Inhibition of the Respiration
Test item concentration [mg a.i./L] |
Replicate |
Oxygen Uptake Rate [mg O2/L·h] |
Inhibition [%] |
Mean inhibition [%] |
Control |
1 2 3 4 5 6 |
31.6 31.6 30.4 28.8 29.6 30.4 |
--- |
--- |
10 |
1 2 3 4 5 |
30.4 30.4 30.0 30.4 29.6 |
0 0 1 0 3 |
|
32 |
1 2 3 4 5 |
26.4 26.4 25.2 26.0 26.0 |
13 13 17 14 14 |
14 |
100 |
1 2 3 4 5 |
18.0 18.4 18.8 18.8 18.0 |
41 39 38 38 41 |
39 |
Oxygen Uptake Rates and Specific Respiration Rates of theTotal Respirationof the Control
|
Repl. |
Oxygen |
Oxygen Uptake Rate RT |
Specific Respiration Rate Rs |
|
|
[mg O2/L] |
[mg O2/(L×h)] |
[mg O2/g×h] |
Control |
1 |
7.04 |
37.7 |
24.4 |
2 |
7.07 |
37.4 |
24.2 |
|
3 |
6.66 |
36.0 |
23.3 |
|
4 |
7.56 |
34.3 |
22.2 |
|
5 |
5.80 |
32.9 |
21.3 |
|
6 |
7.53 |
32.5 |
21.0 |
|
Mean value |
35.1 |
22.7 |
||
Standard deviation |
2.1 |
|||
CV [%] |
5.8 |
*) determined at start of the measurement
Repl. = Replicate ; CV = Coefficient of variation
Oxygen Uptake Rates and Inhibition of the Total Respiration of the Test Item Concentrations
Test Item Concentration |
Rep |
Oxygen |
Oxygen Uptake Rate RT |
Inhibition |
Mean Inhibition |
[mg/L] |
|
[mg O2/L] |
[mg O2/(L×h)] |
[%] |
[%] |
1 |
1 |
7.30 |
34.8 |
1 |
-1 |
2 |
7.34 |
36.6 |
-4 |
||
3 |
7.00 |
36.0 |
-3 |
||
4 |
7.36 |
34.6 |
1 |
||
5 |
6.58 |
34.8 |
1 |
||
3.16 |
1 |
5.92 |
34.7 |
1 |
1 |
2 |
7.00 |
37.4 |
-7 |
||
3 |
6.83 |
33.9 |
3 |
||
4 |
6.68 |
34.7 |
1 |
||
5 |
5.68 |
32.6 |
7 |
||
10 |
1 |
7.31 |
27.6 |
21 |
20 |
2 |
7.72 |
29.9 |
15 |
||
3 |
6.64 |
28.1 |
20 |
||
4 |
7.44 |
28.1 |
20 |
||
5 |
6.70 |
26.9 |
23 |
||
31.6 |
1 |
8.25 |
16.3 |
54 |
57 |
2 |
8.12 |
15.1 |
57 |
||
3 |
8.18 |
14.7 |
58 |
||
4 |
7.80 |
16.0 |
54 |
||
5 |
7.59 |
14.0 |
60 |
||
100 |
1 |
8.61 |
4.4 |
87 |
87 |
2 |
8.95 |
4.5 |
87 |
||
3 |
8.39 |
4.8 |
86 |
||
4 |
8.38 |
4.4 |
87 |
||
5 |
8.29 |
3.4 |
90 |
*) determined at start of the measurement
Repl. = Replicate
Oxygen Uptake Rates of the Heterotrophic Respiration of the Control Replicates with ATU
|
Rep |
Oxygen |
Oxygen Uptake Rate RH |
|
|
[mg O2/L] |
[mg O2/(L×h)] |
Control |
1 |
8.64 |
16.2 |
2 |
8.20 |
14.1 |
|
3 |
8.29 |
15.5 |
|
4 |
8.57 |
13.5 |
|
5 |
8.38 |
12.1 |
|
6 |
8.26 |
12.2 |
|
Mean value |
13.9 |
||
Standard deviation |
1.54 |
||
CV [%] |
11.1 |
*) determined at start of the measurement
CV = Coefficient of variation
Oxygen Uptake Rates and Inhibition of theHeterotrophic Respiration
Test Item |
Rep |
Oxygen [mg O2/L] |
Oxygen Uptake Rate RH |
Inhibition |
Mean Inhibition |
1 + ATU |
1 |
8.67 |
15.7 |
-13 |
-13 |
2 |
8.48 |
16.9 |
-22 |
||
3 |
8.27 |
16.7 |
-20 |
||
4 |
8.31 |
16.1 |
-16 |
||
5 |
7.68 |
13.4 |
4 |
||
3.16 + ATU |
1 |
8.49 |
15.6 |
-12 |
-10 |
2 |
8.12 |
14.9 |
-7 |
||
3 |
7.81 |
15.7 |
-13 |
||
4 |
9.15 |
15.6 |
-12 |
||
5 |
7.84 |
15.0 |
-8 |
||
10 + ATU |
1 |
8.87 |
12.5 |
10 |
11 |
2 |
8.57 |
12.4 |
11 |
||
3 |
8.36 |
12.3 |
12 |
||
4 |
8.51 |
12.4 |
11 |
||
5 |
8.23 |
12.2 |
12 |
||
31.6 + ATU |
1 |
8.99 |
5.4 |
61 |
60 |
2 |
8.71 |
5.7 |
59 |
||
3 |
8.11 |
5.9 |
58 |
||
4 |
8.65 |
6.0 |
57 |
||
5 |
8.60 |
4.8 |
65 |
||
100+ ATU |
1 |
9.32 |
0.8 |
94 |
94 |
2 |
9.20 |
0.7 |
95 |
||
3 |
8.89 |
1.1 |
92 |
||
4 |
8.87 |
0.6 |
96 |
||
5 |
8.80 |
0.7 |
95 |
*) determined at start of the measurement
CalculatedOxygen Uptake Rates of theNitrificationof the Control
|
Rep |
Oxygen Uptake Rate RN |
|
|
[mg O2/(L×h)] |
Control |
1 |
21.5 |
2 |
23.3 |
|
3 |
20.5 |
|
4 |
20.8 |
|
5 |
20.8 |
|
6 |
20.3 |
|
Mean value |
21.2 |
|
Standard deviation |
1.0 |
|
CV [%] |
4.8 |
CV = Coefficient of variation
Oxygen Uptake Rates and Inhibition of theNitrification
Test Item |
Rep |
Oxygen Uptake Rate RN |
Inhibition |
Mean Inhibition |
1 |
1 |
19.0 |
10 |
8 |
2 |
20.8 |
2 |
||
3 |
20.2 |
5 |
||
4 |
18.8 |
11 |
||
5 |
19.0 |
10 |
||
3.16 |
1 |
19.3 |
9 |
9 |
2 |
22.0 |
-4 |
||
3 |
18.5 |
13 |
||
4 |
19.3 |
9 |
||
5 |
17.2 |
19 |
||
10 |
1 |
15.2 |
28 |
26 |
2 |
17.5 |
17 |
||
3 |
15.7 |
26 |
||
4 |
15.7 |
26 |
||
5 |
14.5 |
31 |
||
31.6 |
1 |
10.7 |
49 |
54 |
2 |
9.5 |
55 |
||
3 |
9.1 |
57 |
||
4 |
10.4 |
51 |
||
5 |
8.4 |
60 |
||
100 |
1 |
3.6 |
83 |
83 |
2 |
3.7 |
82 |
||
3 |
4.0 |
81 |
||
4 |
3.6 |
83 |
||
5 |
2.6 |
88 |
Description of key information
3 h EC10 = 6.34 mg a.i./L, EC50 = 26.1 mg a.i./L (OECD TG 209; RL1; GLP)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 26.1 mg/L
- EC10 or NOEC for microorganisms:
- 6.34 mg/L
Additional information
In a 3 hour toxicity study conducted according to OECD Guideline 209 (2010), the cultures of activated sludge of a predominantly domestic sewage treatment plant were exposed toQuarternary ammonium compounds, tri-C8-C10-alkylmethyl, Me sulfatesat nominal concentrations of 0 (control), 1, 3.16, 10, 31.6, and 100 mg a.i./L mg/L under static conditions.
The batch of activated sludge was checked for sensitivity by testing the reference substance Copper (II) sulphate pentahydrate.
The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the controI respiration rates. The mean inhibition of respiration for the test item replicates ranged from -1% to 87%
The 3 h NOEC of C12-18 alkyldimethyl ammonium chloride is 3.16 mg a.i./L. The 3 h EC10 is 6.34 mg a.i./L, and the EC50 is 26.1 mg a.i./L.
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