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EC number: 949-117-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 31 Jan - 28 Feb 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- Version 1992
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Qualifier:
- according to guideline
- Guideline:
- other: US EPA OCSPP 835.3110
- Version / remarks:
- Paragraph (q)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Date of certificate: 19.11.2018 (The Department of Health of the Government of the United Kingdom)
Test material
- Reference substance name:
- Reaction product of castor oil with glycerol
- EC Number:
- 949-117-7
- Molecular formula:
- not applicable, substance is UVCB
- IUPAC Name:
- Reaction product of castor oil with glycerol
- Test material form:
- liquid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, procedure): final effluent stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK; sample of effluent was filtered through coarse filter paper (first approximate 200 mL discarded)
- Storage conditions: maintained on aeration in a temperature controlled room at temperatures of between 20 and 21 °C
- Concentration of sludge: inoculum at a rate of 1% v/v in test vessels
- Initial cell/biomass concentration: 5 mL inoculum per 500 ml test volume
- Water filtered: yes
- Type and size of filter used, if any: coarse filter paper - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium according to OECD guideline
- Test temperature: 20 - 21 °C
- pH: 7.4 - 8.0
- pH adjusted: no
- Continuous darkness: yes
- Other: test was conducted in diffuse light
TEST SYSTEM
- Culturing apparatus: 500 ml amber glass bottles sealed with a sensor head/CO2 trap immersed in a temperature controlled water bath
- Number of culture flasks/concentration: 3 replicates for test item
- Method used to create aerobic conditions: stirring of test vessels with a magnetically coupled stirrer
- Measuring equipment: CES Multi-Channel Aerobic Respirometer
- Test performed in closed vessels due to significant volatility of test substance: yes
- Details of trap for CO2 and volatile organics if used: CO2 is absorbed into an ethanolamine solution (50% v/v) causing a net reduction in gas pressure within the sample flask, pressure reduction triggers the electrolytic process, generating oxygen and restoring the pressure in the sample flask. The magnitude of the electrolyzing current and the duration of the current is proportional to the amount of oxygen supplied to the micro-organisms
SAMPLING
- Sampling frequency: daily
- Sampling method: data generated from the respirometer’s own battery backed memory was collected on the hard disk drive of a non-dedicated computer
- Other: on day 28 two inoculum controls, one procedure control, two test item and one toxicity control vessel were sampled for pH analysis
CONTROL AND BLANK SYSTEM
- Inoculum blank: 3 replicates
- Abiotic sterile control: none
- Toxicity control: 2 replicates
- Other: 2 replicates for positive control
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 60
- Sampling time:
- 28 d
- Remarks on result:
- other: Mean of two replicates
BOD5 / COD results
- Results with reference substance:
- Aniline attained 69% biodegradation after 14 days and 74% biodegradation after 28 days
Any other information on results incl. tables
Table 1. Percentage Biodegradation Values
Day |
Biodegradation (%) |
||||
Procedure Control |
Test Item |
Toxicity Control |
|||
R1 |
R2 |
Mean |
|||
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
1 |
2 |
0 |
16 |
16 |
16 |
6 |
3 |
0 |
25 |
25 |
25 |
8 |
4 |
2 |
29 |
29 |
29 |
18 |
5 |
16 |
32 |
31 |
32 |
28 |
6 |
24 |
33 |
32 |
33 |
41 |
7 |
36 |
36 |
34 |
35 |
49 |
8 |
57 |
37 |
35 |
36 |
51 |
9 |
63 |
39 |
37 |
38 |
51 |
10 |
64 |
40 |
38 |
39 |
52 |
11 |
66 |
42 |
40 |
41 |
53 |
12 |
67 |
44 |
43 |
44 |
54 |
13 |
68 |
46 |
46 |
46 |
54 |
14 |
69 |
47 |
48 |
48 |
55 |
15 |
70 |
49 |
49 |
49 |
55 |
16 |
70 |
51 |
50 |
51 |
56 |
17 |
71 |
53 |
50 |
52 |
57 |
18 |
72 |
54 |
51 |
53 |
57 |
19 |
72 |
55 |
51 |
53 |
58 |
20 |
72 |
56 |
52 |
54 |
59 |
21 |
73 |
56 |
52 |
54 |
60 |
22 |
73 |
57 |
53 |
55 |
61 |
23 |
73 |
58 |
53 |
56 |
62 |
24 |
74 |
59 |
54 |
57 |
63 |
25 |
74 |
60 |
54 |
57 |
64 |
26 |
74 |
61 |
55 |
58 |
65 |
27 |
74 |
62 |
56 |
59 |
66 |
28 |
74 |
63 |
57 |
60 |
66 |
Table 2: Validity criteria for OECD 301F
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
< 20% |
Yes |
Percentage degradation of the reference compound reached the pass level by day 14 (≥ 60%). |
69 % |
Yes |
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d. |
55% |
Yes |
The oxygen uptake of the inoculum blank is normally 20-30 mg O2/L and should not be greater than 60 mg/L in 28 days. |
25.53 mg O2/L at 28 d |
Yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- See Table 2 in "any other information on results incl. tables"
- Interpretation of results:
- readily biodegradable, but failing 10-day window
- Remarks:
- As the test item was a UVCB, the 10-day window criteria does not apply and therefore the test item can be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301F.
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