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EC number: 431-540-9 | CAS number: 170573-32-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- adult fish: sub(lethal) effects
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From February 26, 2002 to June 08, 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- HPLC/MS
- Details on sampling:
- Analytical report 798164 (Biedermann, 2002)
The test substance (stock solution), the test substance solutions and the water (and solvents) (analytical blanks) were analysed. The stock solution (in acetonitrile/purified water) and its dilutions were used to calibrate the HPLC-system. The test substance dissolved in a mixture of acetonitrile/purified water was further diluted with test water and analysed by HPLC. The samples obtained from biological tests (taken on Days 3, 7, 10, 13, 16 and 21 of the exposure period) at different concentration levels were analysed by HPLC with MS detection. Analysis was performed in duplicate. - Vehicle:
- no
- Details on test solutions:
- Aqueous solutions were prepared by dispersing the test substance in test water by ultrasonic treatment. Since the aqueous solutions were not stable for longer time periods, they were renewed regularly (every 2 or 3 days) during the test period.
Aqueous application solutions were first warmed up to obtain a homogeneous liquid and 5 different solutions were prepared by dispersing the test substance in 5 liter purified water each. They were then treated ultrasonically for 45 min and intensively stirred for 15 min to obtain homogeneous dispersions of the test substance in test water. The solutions were prepared at Days 0, 3, 8, 10, 13, 15, 17 and 20. The stock solutions were then added in the aquaria. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- Source: P. Hohler, trout breeding station Zeiningen, CH-4314 Zeiningen, Switzerland.
Acclimatization: at least one week prior to the test
Water temperature: 14 - 15“C
Light conditions: a 16-hour light to 8-hour darkness photoperiod
Diet (prior test): HOKOVIT 502 (adapted to the number of fish)
Number: 10 fish
Mean body length at the start of the test: 5.3 +/- 0.4 cm
Mean body wet weight: 1.5 +/- 0.2 g - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 112-182 mg/L as CaCO3
- Test temperature:
- 14-15°C
- pH:
- 7.6-8.2
- Dissolved oxygen:
- 8.6 mg/L or higher (>60% oxygen concentration)
- Nominal and measured concentrations:
- 0, 0.5, 1.6, 5.0, 16 and 50 mg/L (nominal) (chosen based on an acute toxicity test with rainbow trout, a range-finding test and a pre-experiment.
- Details on test conditions:
- - 60-litre flow-through glass-aquarium with 10 fish
- Reference substance (positive control):
- no
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 1.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: body length and wet weight
- Remarks on result:
- other: 1.2 mg/L (measured)
- Key result
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: body length and wet weight
- Remarks on result:
- other: 2.5 mg/L (measured)
- Key result
- Duration:
- 21 d
- Dose descriptor:
- other: LLC
- Effect conc.:
- ca. 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 27 mg/L (measured)
- Details on results:
- Analytical results for test substance concentrations:
- Freshly prepared application solution samples: 93 - 118% of the nominal values (correct preparation)
- Aged application soltuions: 95 - 123% of nominal values (stability)
- In test media: 14 - 121% (inhomogeneous distribution)
- Measured concentrations: 1.2 mg/L (1.6 mg/L nominal), 2.5 mg/L (5.0 mg/L nominal), and 27 mg/L (50 mg/L nominal).
Biological results:
- Mortality: no mortality was observed in the groups 0, 1.6, 5.0 and 16.0 mg/L (nominal). At 50 mg/L, all fish were dead on Day 15
- Visible abnormalities: they were observed in the groups 16.0 and 50.0 mg/L (nominal) (from Day 16 and from Day 6, respectively)
- Measured mean body length and body wet weight: they were significabtly reduced starting at 5.0 mg/L (nominal) at the end of the test period - Reported statistics and error estimates:
- Parameters were evaluated on significant differences to the control values by the multivariate statistical Williams-test.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the nominal 21 d NOEC of the test substance in Rainbow trout was 1.6 mg/L (corresponding to 1.2 mg/L measured).
- Executive summary:
A study was conducted to determine the long-term toxicity of the test substance, isoC18 MIPA (96% active), to fish according to OECD Guideline 204, in compliance with GLP. Rainbow trout (Oncorhynchus mykiss) were exposed to the test substance (dispersed in the test medium by ultrasonication) at concentrations of 0, 0.5, 1.6, 5.0, 16.0 and 50.0 mg/L (nominal) for 21 d under flow-through conditions. Samples for analytical confirmation were taken from the freshly prepared solutions, application solutions and test water every 2 or 3 d and analysed by HPLC with MS detection. Mortality, visible parameters and water parameters were recorded throughout the exposure period. The average concentration found in application samples was 103%. Samples diluted in test water showed an average concentration of 92%, so no correction for possible losses during the analytical procedure was necessary. The reported biological results were related to the total mean measured test substance concentrations. These were 1.2 mg/L (1.6 mg/L nominal), 2.5 mg/L (5.0 mg/L nominal) and 27 mg/L (50 mg/L nominal). No mortality was observed up to 16.0 mg/L. At 50 mg/L, all fish were dead on Day 15. Visible abnormalities were observed at 16.0 and 50.0 mg/L. Finally, measured mean body length and wet weight of the body were significantly reduced starting at 5.0 mg/L (nominal) at the end of the test period. Under the study conditions, the nominal 21 d NOEC of the test substance in Rainbow trout was 1.6 mg/L (corresponding to 1.2 mg/L measured) (Peither, 2002).
Reference
None.
Description of key information
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1.2 mg/L
Additional information
A study was conducted to determine the long-term toxicity of the test substance, isoC18 MIPA (96% active), to fish according to OECD Guideline 204, in compliance with GLP. Rainbow trout (Oncorhynchus mykiss) were exposed to the test substance (dispersed in the test medium by ultrasonication) at concentrations of 0, 0.5, 1.6, 5.0, 16.0 and 50.0 mg/L (nominal) for 21 d under flow-through conditions. Samples for analytical confirmation were taken from the freshly prepared solutions, application solutions and test water every 2 or 3 d and analysed by HPLC with MS detection. Mortality, visible parameters and water parameters were recorded throughout the exposure period. The average concentration found in application samples was 103%. Samples diluted in test water showed an average concentration of 92%, so no correction for possible losses during the analytical procedure was necessary. The reported biological results were related to the total mean measured test substance concentrations. These were 1.2 mg/L (1.6 mg/L nominal), 2.5 mg/L (5.0 mg/L nominal) and 27 mg/L (50 mg/L nominal). No mortality was observed up to 16.0 mg/L. At 50 mg/L, all fish were dead on Day 15. Visible abnormalities were observed at 16.0 and 50.0 mg/L. Finally, measured mean body length and wet weight of the body were significantly reduced starting at 5.0 mg/L (nominal) at the end of the test period. Under the study conditions, the nominal 21 d NOEC of the test substance in Rainbow trout was 1.6 mg/L (corresponding to 1.2 mg/L measured) (Peither, 2002).
As discussed with ECHA in the frame of a Dossier Improvement Action Plan (DIAP), should the planned new water solubility testing according to OECD Guideline 105 result in a water solubility for the substance <1 mg/L, additional long-term toxicity testing in fish will be conducted with representative short- and a long chain FAA substances.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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