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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2019
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Batch number : 8613
Manufacturing date : October 25, 2018

Test item physical state : Slight yellow powder

Reception date : November 26, 2018
Theoretical Oxygen Demand : ThODNH4 = 2.416 mg O2 / mg test item
Theoretical Oxygen Demand : ThODNO3 = 2.482 mg O2 / mg test item
Storage conditions : Ambiant temperature ( 20 ± 5°C), Hydroscopic and keep away from light
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
The inoculum was a fresh sample of activated sludge and was collected from the aeration tank of a sewage treatment plant receiving predominantly domestic sewage .

Laboratory preparation:
A filtration through a fine sieve (about 1 mm) was performed in order to remove coarse particles. After removal of any coarse particles, the sludge was washed by decantation in a mineral medium until sludge was considered free from excess substrate or inhibitor.

Inoculum pre-conditionning : Pre-conditioning consisted in aerating sludge (in mineral medium) for 5-7 days before the test and at the test temperature.
Sample site : Water treatment plant "STEP de Lille", 33290 Blanquefort (France) Tank 1
Date of sampling : January 24, 2019
Inoculum physico-chemical characteristic:
Suspended solid: 2.9 g/L
pH : 5.9
Dissolved oxygen: 9.1 mg/L
Activated sludge preparation
1: Filtration through a 1 mm sieve
2: 3 Successive decantations into mineral medium
3: Activated sludge aeration during 5 days


Duration of test (contact time):
ca. 28 d
Parameter followed for biodegradation estimation:
O2 consumption
Key result
Parameter:
% degradation (O2 consumption)
Value:
ca. 79.03
Sampling time:
28 d
Key result
Parameter:
% degradation (O2 consumption)
Remarks:
10 day window
Value:
ca. 76.93
Sampling time:
13 d
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Executive summary:

The readily biodegradability of the test substance was assessed according to the OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test).

The test substance is readily biodegradable.

Description of key information

The readily biodegradability of the test substance was assessed according to the OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test).

The test substance is readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable
Type of water:
freshwater

Additional information