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EC number: 225-533-8 | CAS number: 4904-61-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Specific investigations: other studies
Administrative data
Link to relevant study record(s)
- Endpoint:
- biochemical or cellular interactions
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Principles of method if other than guideline:
- details see reference
- GLP compliance:
- no
- Type of method:
- in vivo
- Endpoint addressed:
- basic toxicokinetics
- Species:
- guinea pig
- Route of administration:
- dermal
- Vehicle:
- unchanged (no vehicle)
- Details on exposure:
- 2 ml of 1,5,9-cyclododecatriene were applied to both flanks of guinea pigs (5 replicates) three times on alternate days. Three series of control experiments involving a total of 151 untreated animals were performed.
- Control animals:
- yes
- Details on results:
- The epidermis/dermis dry weight ratio was substantially increased, and there was also an effect on arginase activity:
- epidermis/dermis dry weight ratio (test/control) = 3.8
- arginase activity (test/control) = appr. 2.3 - Endpoint:
- biochemical or cellular interactions
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- see reference
- GLP compliance:
- not specified
- Type of method:
- in vivo
- Endpoint addressed:
- basic toxicokinetics
- Species:
- mouse
- Strain:
- other: ICR/Ha mice
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- Age: 7 weeks
Number: 5-10 animals per group - Route of administration:
- oral: feed
- Details on exposure:
- Control: diet
TREATMENT
- 30 or 50 µmol/g in diet for 2 weeks - Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- 2 weeks
- Frequency of treatment:
- test item in feed
- Dose / conc.:
- 30 other: µmol/g
- Remarks:
- in diet (nominal concentration)
- Dose / conc.:
- 50 other: µmol/g
- Remarks:
- in diet (nominal concentration)
- No. of animals per sex per dose:
- Number: 5-10 animals per group
- Control animals:
- yes, concurrent no treatment
- Examinations:
- EXAMINATIONS
- Removal and homogenization of liver, forestomach, and mucosa from small bowel
- GST activity determination using 1-chloro-2,4-dinitrobenzene as substrate - Details on results:
- None of the compounds elicited increased GST activity in the forestomach. C6 ring compounds showed no significant effect. C12 ring compounds were more effective than C8 ring compounds with a decrease in activity in the order: unsaturated > epoxide > alcohol > saturated. With trans-trans-cis-1,5,9-cyclododecatriene as test substance, the GST activity in the livers was increased 3.79-, 3.78- and 3.82-fold, respectively, in three experiments. The increases in the intetinal GST activity were of similar magnitude.
Referenceopen allclose all
The morphological effect increases with molecular weight of alicyclic
hydrocarbons, saturated and unsaturated substances showing similar effects.
-----------------------------------------------------------
Test compound Liver Small Bowel Mucosa
Specific Activity Specific Activity
------------------------------------------------------------
None 1.96 +- 0.16 0.61 +- 0.04
Cyclododecatriene 7.42 +- 0.53* 2.77 +- 0.29*
None 2.40 +- 0.10 0.65 +- 0.01
Cyclododecatriene 9.06 +- 0.04* 1.74 +- 0.15*
None 1.39 +- 0.05 0.27 +- 0.01
Cyclododecatriene 5.31 +- 0.23* 1.10 +- 0.09*
* = p <0.005
Description of key information
1984: No guideline followed; pre-GLP; 5-10 ICR/Ha mice; 30 and 50 µmol/g diet; GST activity in liver and intestinal mucosa enhanced (K2)
1971: No guideline followed; pre-GLP; Guinea pigs; undiluted test substance; arginase activity was increased (K4)
Additional information
In an in vivo study using guinea pigs, morphological changes and alterations of the epidermal arginase activity were investigated. The term arginase was used for the soluble enzyme L-arginase amidinohydrolase or L-arginase ureahydrolase. Two ml of the test substance were applied to both flanks of guinea pigs (5 replicates) three times on alternate days. Three series of control experiments involving a total of 151 animals were performed. The epidermis/dermis dry weight ratio was substantially increased, and there was also an effect on arginase activity; epidermis/dermis dry weight ratio (test/control) was 3.8 and arginase activity (test/control) was approximately 2.3 (1971, K4).
In an in vivo study using female ICR/Ha mice, effects on glutathione S-transferase (GST) activity in the liver, intestinal mucosa, and the forestomach of exposed animals were determined. Therefore, females were assigned to groups of 5-10 mice. The control group received concurrent feed without the test compound. Treatment groups were divided into low and high dose, i.e. 30 and 50 µmol/g of the test substance in the diet. The duration of exposure was two weeks. After treatment, mice were sacrificed and livers, forestomach, and the mucosa from the small bowel removed. With trans-trans-cis-1,5,9-cyclododecatriene as test substance, the GST activity in livers was increased 3.79-, 3.78-, and 3.82-fold, respectively, in three experiments. The increased in the intestinal GST activity were of similar magnitude. No significant differences in the activity of GST in the forestomach of experimental animals was observed compared to the control (1984, K2).
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