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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
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Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2017-08-23 to 2017-09-22
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Bis and tris and tetra{N-[(2-ethylanilino) or 2,4(or 2,5 or 2,6)-dimethylanilino]-N’-[(2-ethylanilino) or 2,4(or 2,5 or 2,6)-dimethylanilino] methaniminium} [phthalocyanine(bis and tris and tetra)sulfonato-κ4N29,N30,N31,N32]cuprate(II)
- Cas Number:
- 265115-84-2
- Molecular formula:
- C32H16-τN8Cu(SO3C17H22N3)τ
- IUPAC Name:
- Bis and tris and tetra{N-[(2-ethylanilino) or 2,4(or 2,5 or 2,6)-dimethylanilino]-N’-[(2-ethylanilino) or 2,4(or 2,5 or 2,6)-dimethylanilino] methaniminium} [phthalocyanine(bis and tris and tetra)sulfonato-κ4N29,N30,N31,N32]cuprate(II)
- Test material form:
- solid: particulate/powder
1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: activated sludge from the municipal sewage treatment plant, 31137 Hildesheim, Germany: it receives predominantly municipal sewage and hardly any industrial chemical waste. Receipt the 2017-08-06.
- Pretreatment: The activated sludge was washed twice with chlorine free tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2 ½ hours. Thereafter the sludge was homogenized with a blender. After sedimentation the supernatant was decanted and maintained in an aerobic condition by aeration with CO2 free air until test start (7 days). 10 mL/L of this mixture were used to initiate inoculation.
- Initial cell/biomass concentration: Approx. 1.31 x 10e7 CFU/L in the vessel. - Duration of test (contact time):
- ca. 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- ca. 17 mg/L
- Based on:
- test mat.
- Initial conc.:
- ca. 0.612 other: mg C/mg
- Based on:
- TOC
- Initial conc.:
- ca. 2.25 other: mg CO2/ mg test item
- Based on:
- ThCO2
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral salts medium, according to 301B/CO2 Evolution test.
- Test temperature: 22 ± 2°C
- pH: from 7.71 to 7.76 on Day 28.
- Aeration of dilution water: 30 - 100 mL/min.
- Continuous darkness: low light conditions (brown glass bottles)
TEST SYSTEM
- Test vessels: 5000 mL brown glass vessel containing 3000mL of test medium.
- Dispersion treatment: continous stirring
- Application: once at test start
- Number of culture flasks/concentration:
* 2 replicates for the inoculum control
* 1 repicate for the functional control
* 2 replicates for the test item
* 1 replicate for the toxicity control
- Method used to create aerobic conditions: CO2-free air bubbled through the solution at a rate of 30-100 mL/minute.
- Measuring equipment:
pH-Meter, Multi 350i, WTW
Thermohygrograph, LUFFT
Flow meter, Typ DK 800 PV, KROHNE DUISBURG
Medo Compressor, REBIE
- Test performed in open system: yes
- Details of trap for CO2 and volatile organics if used: The produced carbon dioxide is absorbed into the barium hydroxide solution.
Three gas wash bottles, containing 100mL of a 0.0125 mol/L barium hydroxide solution are placed closed to the test vessels. Determination of CO2 carried out by tittration with the barium hydroxide solution at 0.0125 mol/l. For each titration the first gas wash bottle was removed and a new bottle was connected to the last one. Back titration of the residual Ba(OH)2 with 0.05 N HCl was carried out three times a week during the first ten days and thereafter twice weekly.
SAMPLING
- Sampling frequency: D1, D4, D6, D8; D11, D14, D18, D22, D25, D28 and D29.
CONTROL AND BLANK SYSTEM
* 2 replicates for the inoculum control
* 1 repicate for the functional control
* 2 replicates for the test item
* 1 replicate for the toxicity control
- Inoculum control: test medium without test and/or reference item
- Functional control: sodium benzoate at 20 mg/L
- Toxicity control: test item at 17 mg/L and sodium benzoate at 20 mg/L
Reference substance
- Reference substance:
- other: sodium benzoate
Results and discussion
- Test performance:
- The validity criteria were fulfilled according to the guideline:
•The total CO2 evolution in the inoculum control at the end of the test was < 40 mg CO2/L by day 28 ( 21.7 mg CO2/L on day 28).
•The degradation of the functional control reached the pass level of 60% by day 6 (degradation: 62% on day 6).
•The differences of extremes of replicate values of removal of the test item at the end of the test was less than 20% (1% difference on day 28).
•The degradation of the toxicity control reached the pass level of 25% by day 6 (degradation: 32% on day 6).
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 1
- Sampling time:
- 28 d
- Details on results:
- Colony forming units (CFU) of the inoculum for the Modified Sturm Test were determined prior to test start by standard dilution plate count: approx. 1.31x109 CFU/L, corresponding to approx. 1.31x107 CFU/L in the test vessel.
The adaptation phase of the functional control changed within 4 days into the degradation phase (degradation of 10%). The course of the degradation was rapid and the functional control reached the pass level of 60% within 6 days and a maximum biodegradation of 88% on day 28. The validity criterion degradation of 60% after 14 days is fulfilled.
Any other information on results incl. tables
Table1: Biodegradation of the Test Item in comparison to the Functional control and the toxicity control
|
Biodegradation [%] |
|||
Study Day [d] |
||||
6 |
14 |
21 |
28 |
|
Test Item, First Replicate |
0 |
0 |
0 |
1 |
Test Item, Second Replicate |
0 |
0 |
0 |
0 |
Functional Control |
62 |
83 |
87 |
88 |
Toxicity Control |
32 |
44 |
45 |
47 |
Table 2: CO2-Production and Biodegradation after 28 Days
CO2-Production |
Functional control |
Test Item |
Toxicity control test item + |
|
1 |
2 |
|||
Net [mg/3 L] |
113.1 |
1.2 |
0.2 |
114.7 |
[mg/L] |
37.7 |
0.4 |
0.1 |
38.2 |
Theor. [mg/3 L] |
127.8 |
114.8 |
242.6 |
|
[mg/L] |
42.6 |
38.3 |
80.9 |
|
Degradation [%] |
88 |
1 |
0 |
47 |
Table 3: CO2-Production and Biodegradation in the Inoculum Control, the Functional Control and the Toxicity Control
Study Day |
Date |
Inoculum |
Functional Control |
Toxicity Control |
||||
[mg CO2/3 L] |
[mg CO2/3 L] |
Degr. |
[mg CO2/3 L] |
Degr. |
||||
mv |
Gross |
Net Sum |
[%] |
Gross |
Net Sum |
[%] |
||
1 |
2017-08-25 |
2.5 |
4.1 |
1.6 |
1 |
4.2 |
1.7 |
1 |
4 |
2017-08-28 |
6.3 |
53.4 |
48.7 |
38 |
50.3 |
45.7 |
19 |
6 |
2017-08-30 |
5.4 |
35.9 |
79.2 |
62 |
37.2 |
77.5 |
32 |
8 |
2017-09-01 |
6.5 |
22.1 |
94.8 |
74 |
19.3 |
90.3 |
37 |
11 |
2017-09-04 |
6.2 |
14.0 |
102.6 |
80 |
15.6 |
99.7 |
41 |
14 |
2017-09-07 |
7.1 |
10.9 |
106.4 |
83 |
13.8 |
106.4 |
44 |
18 |
2017-09-11 |
6.6 |
9.6 |
109.4 |
86 |
8.6 |
108.4 |
45 |
22 |
2017-09-14 |
7.7 |
9.2 |
110.9 |
87 |
9.2 |
109.9 |
45 |
25 |
2017-09-18 |
6.7 |
7.6 |
111.8 |
87 |
7.8 |
111.0 |
46 |
28 |
2017-09-21 |
5.2 |
5.9 |
112.5 |
88 |
5.9 |
111.7 |
46 |
29* |
2017-09-22 |
4.8 |
5.4 |
113.1 |
88 |
7.8 |
114.7 |
47 |
Degr. = degradation mv = mean value *) results of last two gas wash bottles
Table 4: CO2-Production and Biodegradation in the Inoculum Control and Test Item Samples
Study Day |
Date |
Inoculum |
Test Item |
||||||
Replicate 1 |
Replicate 2 |
||||||||
[mg CO2/3 L] |
[mg CO2/3 L] |
Degr. |
[mg CO2/3 L] |
Degr. |
|||||
mv |
Gross |
Net Sum |
[%] |
Gross |
Net Sum |
[%] |
|||
1 |
2017-08-25 |
2.5 |
2.2 |
0.0 |
0 |
2.6 |
0.1 |
0 |
|
4 |
2017-08-28 |
6.3 |
5.1 |
0.0 |
0 |
4.8 |
0.1 |
0 |
|
6 |
2017-08-30 |
5.4 |
4.7 |
0.0 |
0 |
4.4 |
0.1 |
0 |
|
8 |
2017-09-01 |
6.5 |
4.4 |
0.0 |
0 |
3.9 |
0.1 |
0 |
|
11 |
2017-09-04 |
6.2 |
4.2 |
0.0 |
0 |
4.3 |
0.1 |
0 |
|
14 |
2017-09-07 |
7.1 |
5.7 |
0.0 |
0 |
5.7 |
0.1 |
0 |
|
18 |
2017-09-11 |
6.6 |
5.4 |
0.0 |
0 |
5.1 |
0.1 |
0 |
|
22 |
2017-09-14 |
7.7 |
6.4 |
0.0 |
0 |
6.2 |
0.1 |
0 |
|
25 |
2017-09-18 |
6.7 |
4.0 |
0.0 |
0 |
6.3 |
0.1 |
0 |
|
28 |
2017-09-21 |
5.2 |
6.4 |
1.2 |
1 |
3.6 |
0.1 |
0 |
|
29* |
2017-09-22 |
4.8 |
4.6 |
1.2 |
1 |
4.9 |
0.2 |
0 |
|
Degr. = degradation mv = mean value *) results of last two gas wash bottles
Table 5: pH-Values on Day 28
Inoculum Control |
Func-tional |
Test Item |
Toxicity Control |
||
No. 1 |
No. 2 |
No.1 |
No. 1 |
No. 2 |
No. 1 |
7.74 |
7.75 |
7.76 |
7.73 |
7.72 |
7.71 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Under the test conditions the test item is classified as not readily biodegradable within the 28 day period of the study.
- Executive summary:
The ready biodegradability of the test item was determined with a non-adapted activated sludge over a test period of 28 days in the Modified Sturm Test. The study was conducted from 2017-08-23 to 2017-09-22, according to OECD 301 B at the test facility. The test item was tested at a concentration of 17 mg/L with 2 replicates corresponding to a carbon content (TOC) of 10.4 mg C/L in the test vessels.The test vessels were incubated at low light conditions and at a temperature of 22 ± 2 °C.
The biodegradation of the test item was followed by titrimetric analysis of the quantity of CO2 produced by the respiration of bacteria. The degradation was stopped on day 28 by acidification of the test solutions. The last titration was made on day 29 after residual CO2 had been purged from the test solutions over a period of 24 hours. The percentage CO2 production was calculated in relation to the theoretical CO2 production (ThCO2) of the test item. The biodegradation was calculated for each titration time.
To check the activity of the test system sodium benzoate was used as functional control. The percentage degradation of the functional control reached the pass level of 60% within 6 days and a maximum biodegradation of 88% on day 28.
In the toxicity control containing both test and reference item a biodegradation of 44% was determined within 14 days and it came to 47% after 28 days. The biodegradation of the reference item was not inhibited by the test item in the toxicity control.
Both test item replicates did not reach the 10% level (beginning of biodegradation) within the 28-day-period of the study. The mean biodegradation on day 28 was 1%.
Under the test conditions the test item is classified as not readily biodegradable within the 28 day period of the study.
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