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EC number: 618-312-6 | CAS number: 898566-17-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016-01-15 to 2016-02-19
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14, 2000.
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: M14KB4863
- Expiration date of the lot/batch: 2017-11-10 (retest date)
- Purity test date: 2015-05-12
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At room temperature
- Stability under test conditions: The test samples were stored in the freezer (≤ -15°C). Storage stability of samples under these conditions was demonstrated in project 509941. - Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: samples for possible analysis were taken form all test concentrations and the control at t=0 and t=48h. 2.0 mL of volume was taken from the approximate centre of the test vessels. At the end of the exposure period, the replicates were pooled at each concentration before sampling
- Sample storage conditions before analysis: the samples were stored in a freezer. Additionally, reserve samples of 2.0 mL were taken for possible analysis - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Due to expected low water solubility of the test substance a saturated solution (SS) was prepared. The preparation of test solutions started with a loading rate of 100 mg/L applying two days of stirring at room temperature in the dark to ensure maximal dissolution of the test item in the tes medium. The resulting aqueous mixture was filtered through a 0.45 µm membrane filter (RC55, Whatman) to remove any non-dissolved material. The filter was pre-conditioned with a small volume of test solution that was discarded to avoid loss of the test item due to adsorption on the filter. The resulting SS was used as the highest test concentration. The lower concentrations were prepared by subsequent dilutions in test medium.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): final test solutions were clear and colourless - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water-flea
- Source: in-house laboratory culture with a known history
- Age at study initiation (mean and range, SD): <24h (from parental daphnids of more than 2 weeks old)
- Method of breeding: 250 newborn daphnids (i.e. less than 3 days old) are placed into 5 litres of medium in an all-glass culture vessel. Medium is M7. After 7 days of cultivation half of the medium is renewed twice a week.
- Feeding during test: no
ACCLIMATION
- Acclimation period: max. 4 weeks
- Acclimation conditions (same as test or not): yes
- Type and amount of food: fresh water algae.
- Feeding frequency: daily
- Health during acclimation (any mortality observed): no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 180 mg/L as CaCO3
- Test temperature:
- 19-20°C
- pH:
- At t= 0h: 7.7
At t= 48h: 8.1 - Dissolved oxygen:
- At t= 0h: 9.2-9.8 mg/L
At t= 48h: 9.1 mg/L - Salinity:
- not relevant
- Nominal and measured concentrations:
- Final test =
Nominal concentrations: 1.0, 10 and 100% of a SS
Nominal concentration tested: 100 mg/L
Measured concentrations at t=0h: < 4 µg/L
Measured concentrations at t=48h: < 4 µg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: beaker
- Material, size, headspace, fill volume: 100 mL, glass beaker filled with 80mL
- Aeration: not during the test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
OTHER TEST CONDITIONS
- Photoperiod: 16h light daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobility was defined as those organisms which are not able to swim within 15 seconds after gentle agitation of the test container and was monitored after 24 and 48 hours of continuous treatment with the test substance.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: x10
- Range finding study: combined/range finding study was performed
- Test concentrations: 1.0, 10 and 100% of a SS prepared at a loading rate of 100 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 100% v/v saturated solution, equivalent to a loading rate of 100 mg/L
- Details on results:
- - mortality of control: none
- Other adverse effects control: no
- Abnormal responses: none - Results with reference substance (positive control):
- - Results with reference substance valid? yes (should lie between 0.3 and 1.0 mg/L)
- Mortality:
- EC50/LC50: 48-h EC50 = 0.38 mg/L with a 95% confidence interval between 0.33 and 0.43 mg/L. The sensitivity of the daphnia was within the range determined with the historical data collected at WIL Research Europe. - Reported statistics and error estimates:
- No EC50 could be calculated because the test item proved to be non-toxic (EC50 > maximum soluble concentration tested)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of T003063 to Daphnia magna was determined in a 48-hour static test according to the OECD guideline 202.
No immobility was observed during the test period of 48 hours in the control and up to and including the highest saturated test concentration of 100 mg/L. At this nominal loading rate of 100 mg/L, no quantifiable amounts of test item were present in the test medium from the start of testing (i.e. actual test concentration was below the lowest calibration solution of 4 µg/L). The 48 hour EC50 for T003063 could not be quantified due to the absence of toxicity up to the highest concentration tested, and was clearly higher than 100 mg/L (nominal loading rate).
Reference
Description of key information
The study of Bouwman (2016), investigating the acute toxicity of T003063 in Daphnia magna according to OECD guideline 202, was considered as the key study for endpoint coverage.
No immobility was observed during the test period of 48 hours in the control and up to and including the highest saturated test concentration of 100 mg/L. At this nominal loading rate of 100 mg/L, no quantifiable amounts of test item were present in the test medium from the start of testing (i.e. actual test concentration was below the lowest calibration solution of 4 µg/L). The 48 hour EC50 for T003063 could not be quantified due to the absence of toxicity up to the highest concentration tested, and was clearly higher than 100 mg/L (nominal loading rate).
Key value for chemical safety assessment
Additional information
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