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EC number: 281-468-5 | CAS number: 83950-14-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23 January 2018 to 07 February 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Analytical samples were taken from all test item concentrations and control at test start and 24 hours (fresh) after 48 hours from aged solutions. Samples (50 mL) were stored deep-frozen (≤ - 18 °C) until analysis. In the analytical laboratory, the samples were thawed to ambient temperature, mixed with 50 mL acetonitrile and shaken well using a flatbed shaker for 15 min. If necessary, the samples were further diluted with acetonitrile/test medium (1:1, v/v) prior to analysis by HPLC-MS/MS.
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Clone V
- Source: in house originating from Federal Environment Agency in Berlin/Germany.
- Age of parental stock: < 24 h
- Feeding during test: none
CULTURE CONDITIONS
- Conditions: single culture (1 daphnid per 100 mL) in Elendt M4 medium. The pH-value of the aerated water was within a range of 6.0 – 9.0. The dissolved oxygen was above 60 % saturation and the total hardness 140 - 250 mg/L (as CaCO3)
- Type and amount of food: single cell green algae (Desmodesmus subsp.)
- Feeding frequency: at least 3 times per week
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 232 mg/L as CaCO3
- Test temperature:
- 18.9- 19.9°C
- pH:
- 7.45-7.92
- Dissolved oxygen:
- 8.8-9.2 mg/L
- Salinity:
- NA
- Nominal and measured concentrations:
- Nominal: 0, 0.0625, 0.125, 0.250, 0.500 and 1.00 mg/L
Measured: 85-103% of nominal - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL all glass covered with glass plate (fill volume 50 mL)
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ELENDT M4 medium according to the guidance
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 light/ 8 dark
- Light intensity: no data
EFFECT PARAMETERS MEASURED : immobility at 0, 24 and 48 hours
RANGE-FINDING STUDY
- Test concentrations: Semi-static at 0, 0.01, 0.1, 1 and 10 mg/L in a closed system
- Results used to determine the conditions for the definitive study: immobility after 24 and 48 h: 5, 0, 0, 100 and 100% - Reference substance (positive control):
- yes
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.492 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- EC50 potassium dichromate 1-2 mg/L
- Reported statistics and error estimates:
- EC50 with Probit analysis using linear max likelihood regression
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 of the substance is 0.492 mg/L
- Executive summary:
In a test according to OECD 202, 20 daphnids per concentration (nominal: 0, 0.0625, 0.125, 0.250, 0.500 and 1.00 mg/L ; measured: 85-103% of nominal) were exposed for 48 hours. Immobility was observed at concentrations of 0.500 mg/L and higher. The EC50 of the substance is 0.492 mg/L (measured).
Reference
Results of the main test, 24 h values
|
Nominal test item concentration [mg/L] |
|||||
|
Control |
0.0625 |
0.125 |
0.250 |
0.500 |
1.00 |
|
Immobilised daphnids after 24 h |
|||||
Group 1 |
1 |
0 |
0 |
0 |
0 |
1 |
Group 2 |
0 |
0 |
0 |
0 |
0 |
1 |
Group 3 |
0 |
0 |
0 |
0 |
0 |
1 |
Group 4 |
0 |
0 |
0 |
0 |
0 |
0 |
Σ |
1 |
0 |
0 |
0 |
0 |
3 |
% |
5 |
0 |
0 |
0 |
0 |
15 |
Results of the main test, 48 h values
|
Nominal test item concentration [mg/L] |
|||||
|
Control |
0.0625 |
0.125 |
0.250 |
0.500 |
1.00 |
|
Immobilised daphnids after 48 h |
|||||
Group 1 |
2* |
0 |
0 |
0 |
5 |
5 |
Group 2 |
0 |
0 |
0 |
0 |
3 |
5 |
Group 3 |
0 |
0 |
0 |
0 |
2 |
5 |
Group 4 |
0 |
0 |
0 |
0 |
2 |
4 |
Σ |
1 |
0 |
0 |
0 |
12 |
19 |
% |
5 |
0 |
0 |
0 |
60 |
95 |
* One daphnid died accidentally and was therefore excluded from evaluation
Description of key information
In a test according to OECD 202, 20 daphnids per concentration (nominal: 0, 0.0625, 0.125, 0.250, 0.500 and 1.00 mg/L ; measured: 85-103% of nominal) were exposed for 48 hours. Immobility was observed at concentrations of 0.500 mg/L and higher. The EC50 of the substance is 0.492 mg/L (measured).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.492 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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