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EC number: 237-424-2 | CAS number: 13780-06-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- No data
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Good-quality study, conducted to GLP. Certain water quality parameters (e.g. hardness, conductivity) were not reported.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 and 100 mg/L (replicates [R] R1-R3 and R4-R6 pooled) at 0 and 72 hours for quantitiative analysis.
- Sampling method: Duplicate samples were taken at 0 and 72 hours
- Sample storage conditions before analysis: Samples were stored at approximately -20 deg C for further analysis if necessary - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 100 mg of test material was dissolved in culture medium and the volume adjusted to 500 mL to give a 200 mg/L stock solution. This was subsequently mixed with algal suspension (500 mL) to give the required test concentration (100 mg/L).
- Eluate: no data
- Differential loading: no data
- Controls: test medium
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): not applicable
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no data - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green alga
- Strain: CCAP 276/20
- Source (laboratory, culture collection): Culture Collection of Algae and Protozoa, Dunstaffnage Marine Laboratory, Oban, Argyll, Scotland
- Age of inoculum (at test initiation): no data
- Method of cultivation: Cultures were maintained in the laboratory by the periodic replenishment of the culture medium
ACCLIMATION
- Acclimation period: yes (duration not specified)
- Culturing media and conditions (same as test or not): The culture was maintained in the laboratory at a temperature of 21+/-1 deg C under conditions of continuous illumination (7000 lux) and constant aeration
- Any deformed or abnormal cells observed: no data - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- As recommended in applicable guideline
- Post exposure observation period:
- Not applicable
- Hardness:
- No data
- Test temperature:
- 24+/-1 deg C
- pH:
- 7.3 (0 hours) - 7.8 (72 hours)
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Conductivity:
- No data
- Nominal and measured concentrations:
- Nominal: 0 and 100 mg/L
Measured: limit concentration was 98.7 mg/L (R1-3) or 99.6 mg/L (R4-6) at 0 hr and 93.3 mg/L (R1-3) or 94.8 mg/L (R4-6) at 72 hr - Details on test conditions:
- TEST SYSTEM
- Test vessel: conical flasks fitted with foam bungs
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glass, 250 mL, no data, 100 mL
- Aeration: no data
- Type of flow-through (e.g. peristaltic or proportional diluter): no data
- Renewal rate of test solution (frequency/flow rate): no data
- Initial cells density: 10,000 cells/mL
- Control end cells density: no data
- No. of organisms per vessel: no data
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): not applicable
GROWTH MEDIUM
- Standard medium used: yes (essentially AAP medium)
- Detailed composition if non-standard medium was used: included Na2SeO3.5H2O (0.000010 mg/L)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: no data
- Total organic carbon: no data
- Particulate matter: no data
- Metals: no data
- Pesticides: no data
- Chlorine: no data
- Alkalinity: no data
- Ca/mg ratio: no data
- Conductivity: no data
- Culture medium different from test medium: no
- Intervals of water quality measurement: no data
OTHER TEST CONDITIONS
- Sterile test conditions: no data
- Adjustment of pH: no data
- Photoperiod: Continuous illumination
- Light intensity and quality: 7000 lux
- Salinity (for marine algae): not applicable
- Other: Constant shaking at 150 rpm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: no data
- Chlorophyll measurement: no data
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable
- Justification for using less concentrations than requested by guideline: limit concentration - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): no data
- Observation of abnormalities (for algal test): no data
- Unusual cell shape: no data
- Colour differences: no data
- Flocculation: nod data
- Adherence to test vessels: no data
- Aggregation of algal cells: no data
- Any stimulation of growth found in any treatment: growth at the limit concentration was 10% higher than that of the control at 72 hr
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no data
- Effect concentrations exceeding solubility of substance in test medium: no data - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- Area under the growth curve at 72 hr was 5.09 x 10^6 for the control and 6.25 x 10^6 for the limit concentration, corresponding to a 23% increase. The growth rate was 0.049 for the control and 0.054 for the limit concentration, corresponding to a 10% increase in growth.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- In a guideline study, to GLP, growth and biomass were unaffected in green algae exposed to freshwater containing sodium nitrite at a nominal limit concentration of 100 mg/L for 72 hours.
- Executive summary:
In an OECD Test Guideline 201 study, conducted according to GLP, sodium nitrite was studied for its ability to inhibit the growth of freshwater Desmodesmus subspicatus (green algae) under static conditions.
The test substance was added to culture medium and the organisms (initial cell density 10,000 cells/mL) exposed at 0 or 100 mg/L (nominal) for 72 hours. There was no inhibitory effect on growth rate or biomass as a result of the treatment; growth at the limit concentration was actually 10% higher than that of the control at 72 hr.
Based on these results, and in the absence of any adverse effects, the 72-hr NOEC (both growth rate and biomass) for the test material was considered to be 100 mg/L. The 72-hr EC50 is evidently somewhat higher than this limit concentration.
Reference
Analysis of the test concentrations at 0 and 72 hours showed measured test concentrations to be near nominal, hence the EC50 values were estimated beased on the nominal test concentrations only. Additional analysis of the test samples was conducted for the presence of sodium nitrate at both 0 and 72 hours in order to determine whether conversion of nitrite to nitrate occurred. The results obtained showed measured test concentrations of less than 1% of nominal for sodium nitrite at 0 hours and 5% of nominal at 72 hours thereby indicating that no conversion occurred.
Mean cell densities for the control were 9.01 x 10^3, 2.74 x 10^4, 5.71 x 10^4 and 3.00 x 10^5 at 0, 24, 48 and 72 hr, respectively. Analogous values for the limit concentration were 7.72 x 10^3, 2.72 x 10^4, 6.68 x 10^4 and 3.71 x 10^5 (at the various respective time points)
Description of key information
No data on calcium nitrite were identified.
In a GLP study conducted according to OECD Test Guideline 201, growth and biomass were unaffected in green algae exposed to freshwater containing sodium nitrite at a nominal limit concentration of 100 mg/L for 72 hours (JAFA, 2005).
Key value for chemical safety assessment
Additional information
No data on calcium nitrite were identified.
In an OECD Test Guideline 201 study, conducted according to GLP, sodium nitrite was studied for its ability to inhibit the growth of freshwater Desmodesmus subspicatus (green algae) under static conditions. The test substance was added to culture medium and the organisms (initial cell density 10,000 cells/mL) exposed at 0 or 100 mg/L (nominal) for 72 hours. There was no inhibitory effect on growth rate or biomass as a result of the treatment; growth at the limit concentration was actually 10% higher than that of the control at 72 hr. Based on these results, and in the absence of any adverse effects, the 72-hr NOEC (both growth rate and biomass) for the test material was considered to be 100 mg/L. The 72-hr EC50 is evidently somewhat higher than this limit concentration (JAFA, 2005).
Sodium nitrite is closely related to calcium nitrite, and is considered a suitable surrogate for read-across for this endpoint. [See read-across justification report in IUCLID section 13 for details.]
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