Naphthalene-2,6-dicarboxylic acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

21 March 2018 - 18 April 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Batch No.of test material:
0012747069
- Expiration date of the lot/batch:
January 24, 2019
- Purity test date:
99.96%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
At ambient temperature
- Stability under test conditions:
stable

Analytical monitoring:

yes

Details on sampling:

- Concentrations: All stock solutions and all test concnetrations
- Sample storage conditions before analysis: All samples were stored in amber glass bottles in the dark at a temperature of ≤ -18°C.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: According to OECD 2000, Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures. (ENV/JM/MONO(2000)6). A stock solution of 100 mg/L was ultrasonicated for 5 min and then stirred for 69 hours and 15 min at ambient temperarue in the dark at 1000 rpm. After setteling, the solution was filtered and the first 150 mL of the filtrate were discarded.
- Evidence of undissolved material: By visual inspection, the filtered stock solution showed no Tyndall effect as an evidence for unsolved test substance

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Clone: M10
- Source: Cultured at the test lab since December 2011
- Age of parental stock: Less than 35 days
- Feeding during test: Daphnids were not fed during the exposure period

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

254 mg/L as CaCO3

Test temperature:

19.8°C

pH:

7.7

Dissolved oxygen:

8.6 mg/L

Conductivity:

674 µS/cm

Nominal and measured concentrations:

Nominal: 100, 56.2, 31.6, 17.7, and 10.0 % of a saturated solution at a loading rate of 100 test item/L and a control
Measured: 9.92, 2.92, 9.92 mg/L determined for the nominal concentrations of 100, 31.6 and 10.0 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: 100 mL glass beakers. fill volume 50-60 mg/L per vessel
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
Elendt medium M4
- Alkalinity: 1.0 mmol/L
- Conductivity: 674 µS/cm
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light: 8 hours dark
- Light intensity: 50-1000lx

EFFECT PARAMETERS MEASURED: Immobility, determined daily

VEHICLE CONTROL PERFORMED: no

RANGE-FINDING STUDY
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 9.92 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Details on results:

- Mortality of control: No
- Other adverse effects control: No

Reported statistics and error estimates:

Probit analysis using linear max. likelihood regression.

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the test, the EC50 of 2,6 -Naphthalene dicarboxylic acid to Daphnia magna is higher than the highest test concentration of 9.92 mg/L of the initially measured test concentration.

Description of key information

Determination of the acute toxicity of 2,6 -Naphthalene dicarboxylic acid to Daphnia magna.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

9.92 mg/L